Harpegnathos saltator (Jerdon s jumping ant)
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15029 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2BUT6|E2BUT6_HARSA Pleckstrin-like proteiny domain-containing family M member 3 OS=Harpegnathos saltator OX=610380 GN=EAI_14169 PE=4 SV=1
MM1 pKa = 7.11 VVLSNFLTPQEE12 pKa = 4.6 GIRR15 pKa = 11.84 HH16 pKa = 5.62 EE17 pKa = 4.32 EE18 pKa = 3.96 QNTTVYY24 pKa = 10.76 INDD27 pKa = 3.7 RR28 pKa = 11.84 EE29 pKa = 4.35 VGKK32 pKa = 8.78 GTFYY36 pKa = 9.91 ITEE39 pKa = 4.36 SVLSWVNSDD48 pKa = 3.51 TQQGFSLEE56 pKa = 4.16 YY57 pKa = 10.02 PHH59 pKa = 7.27 ISLHH63 pKa = 6.32 AISRR67 pKa = 11.84 DD68 pKa = 3.7 EE69 pKa = 3.97 QVHH72 pKa = 5.92 PRR74 pKa = 11.84 QCLYY78 pKa = 11.52 VMVDD82 pKa = 3.56 GKK84 pKa = 11.37 VDD86 pKa = 3.96 LPDD89 pKa = 3.76 MPLLLSPDD97 pKa = 3.35 NSSEE101 pKa = 4.61 NEE103 pKa = 4.42 DD104 pKa = 4.35 DD105 pKa = 6.4 DD106 pKa = 4.81 EE107 pKa = 4.5 DD108 pKa = 4.35 TPITEE113 pKa = 4.36 MRR115 pKa = 11.84 FAPDD119 pKa = 3.05 NTNNLEE125 pKa = 4.9 LMFQAMNACQALHH138 pKa = 7.51 PDD140 pKa = 3.96 PQDD143 pKa = 3.33 SFSDD147 pKa = 3.31 EE148 pKa = 5.23 DD149 pKa = 3.62 IYY151 pKa = 11.56 EE152 pKa = 4.56 DD153 pKa = 3.43 AAEE156 pKa = 4.96 DD157 pKa = 3.49 YY158 pKa = 11.34 GEE160 pKa = 4.04 YY161 pKa = 10.26 SEE163 pKa = 5.55 EE164 pKa = 4.17 VGAGDD169 pKa = 4.62 APYY172 pKa = 10.34 ILPTEE177 pKa = 4.35 QIGTSHH183 pKa = 6.65 NGSEE187 pKa = 4.46 AEE189 pKa = 3.84 EE190 pKa = 4.32 AMDD193 pKa = 3.75 VEE195 pKa = 4.86 AGQFEE200 pKa = 4.57 DD201 pKa = 5.56 AEE203 pKa = 4.32 EE204 pKa = 4.4 DD205 pKa = 3.54 PP206 pKa = 5.23
Molecular weight: 23.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.706
IPC_protein 3.694
Toseland 3.503
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.579
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.617
Nozaki 3.783
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.592
Sillero 3.821
Patrickios 0.846
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.719
Protein with the highest isoelectric point:
>tr|E2BFI9|E2BFI9_HARSA 60S ribosomal protein L40 OS=Harpegnathos saltator OX=610380 GN=EAI_09163 PE=3 SV=1
AA1 pKa = 7.47 GRR3 pKa = 11.84 QAGRR7 pKa = 11.84 QAGRR11 pKa = 11.84 QAGRR15 pKa = 11.84 QAGRR19 pKa = 11.84 QAGRR23 pKa = 11.84 QAGRR27 pKa = 11.84 QAGRR31 pKa = 11.84 QAGRR35 pKa = 11.84 QAGRR39 pKa = 11.84 QAGRR43 pKa = 11.84 QAGRR47 pKa = 11.84 QAGRR51 pKa = 11.84 QAGG54 pKa = 3.46
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.31
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15029
0
15029
6112760
8
14447
406.7
45.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.517 ± 0.026
2.003 ± 0.032
5.538 ± 0.02
6.848 ± 0.038
3.482 ± 0.017
5.397 ± 0.025
2.532 ± 0.012
5.584 ± 0.02
6.231 ± 0.029
9.019 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.359 ± 0.012
4.836 ± 0.02
5.028 ± 0.032
4.258 ± 0.023
5.907 ± 0.022
8.012 ± 0.03
5.995 ± 0.024
6.167 ± 0.021
1.114 ± 0.008
3.171 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here