Madurella mycetomatis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Sordariales incertae sedis; Madurella

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9733 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A175W1V6|A0A175W1V6_9PEZI Acetamidase OS=Madurella mycetomatis OX=100816 GN=MMYC01_205009 PE=4 SV=1
MM1 pKa = 7.75EE2 pKa = 5.57NPDD5 pKa = 3.75PNDD8 pKa = 3.28AVFDD12 pKa = 4.37PEE14 pKa = 3.84LDD16 pKa = 3.52ARR18 pKa = 11.84FSDD21 pKa = 4.0LAMAGGSPTQGEE33 pKa = 4.72TFSNTTTDD41 pKa = 3.45ADD43 pKa = 4.12TSTFWPDD50 pKa = 2.5QDD52 pKa = 3.81TSISADD58 pKa = 3.24PATTKK63 pKa = 10.77FGDD66 pKa = 3.74FDD68 pKa = 5.14ASPQHH73 pKa = 5.95ATTFATQSPGGLNNAEE89 pKa = 4.08LEE91 pKa = 4.49EE92 pKa = 4.54YY93 pKa = 8.31SQHH96 pKa = 5.67MASPAYY102 pKa = 8.14WHH104 pKa = 7.22PEE106 pKa = 3.74SGDD109 pKa = 3.53AGSSSDD115 pKa = 5.25LPFACPYY122 pKa = 9.7EE123 pKa = 4.73GCTNAYY129 pKa = 9.56HH130 pKa = 6.33RR131 pKa = 11.84QCDD134 pKa = 3.9LDD136 pKa = 5.23GEE138 pKa = 4.53DD139 pKa = 4.9AISVTAAAPKK149 pKa = 10.14QRR151 pKa = 11.84TT152 pKa = 3.43

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A175W6P7|A0A175W6P7_9PEZI Heat shock protein sti1 OS=Madurella mycetomatis OX=100816 GN=MMYC01_203500 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84RR3 pKa = 11.84HH4 pKa = 6.5HH5 pKa = 6.52IVPVTTTTAPRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84GGLFSRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84APVKK31 pKa = 10.15VSHH34 pKa = 6.43HH35 pKa = 6.18HH36 pKa = 5.3TTTTTTAPRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84GGLFSRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84APVAVAPRR64 pKa = 11.84HH65 pKa = 5.6HH66 pKa = 6.81KK67 pKa = 9.92RR68 pKa = 11.84HH69 pKa = 5.75ASIGDD74 pKa = 3.95KK75 pKa = 10.64ISGALLRR82 pKa = 11.84LKK84 pKa = 9.84GTLTGRR90 pKa = 11.84PGQKK94 pKa = 9.88AAGTRR99 pKa = 11.84RR100 pKa = 11.84MHH102 pKa = 5.61GTDD105 pKa = 2.52GRR107 pKa = 11.84VAHH110 pKa = 6.8RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84HH114 pKa = 5.79FII116 pKa = 3.73

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9733

0

9733

4853850

52

7603

498.7

54.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.406 ± 0.022

1.212 ± 0.01

5.727 ± 0.015

6.194 ± 0.024

3.556 ± 0.013

7.389 ± 0.026

2.333 ± 0.009

4.476 ± 0.017

4.438 ± 0.023

8.914 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.008

3.337 ± 0.012

6.37 ± 0.032

3.881 ± 0.017

6.624 ± 0.024

7.805 ± 0.025

5.8 ± 0.016

6.293 ± 0.016

1.49 ± 0.009

2.621 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski