Puumala virus (strain Sotkamo/V-2969/81)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Hantaviridae; Mammantavirinae; Orthohantavirus; Puumala orthohantavirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P27313|NCAP_PUUMS Nucleoprotein OS=Puumala virus (strain Sotkamo/V-2969/81) OX=39002 GN=N PE=3 SV=1
MM1 pKa = 7.84SDD3 pKa = 3.19LTDD6 pKa = 3.28IQEE9 pKa = 4.92DD10 pKa = 3.73ITRR13 pKa = 11.84HH14 pKa = 4.34EE15 pKa = 4.1QQLIVARR22 pKa = 11.84QKK24 pKa = 11.27LKK26 pKa = 10.1DD27 pKa = 3.35AEE29 pKa = 4.31RR30 pKa = 11.84AVEE33 pKa = 4.02VDD35 pKa = 3.33PDD37 pKa = 3.98DD38 pKa = 4.16VNKK41 pKa = 9.1NTLQARR47 pKa = 11.84QQTVSALEE55 pKa = 4.66DD56 pKa = 3.13KK57 pKa = 11.04LADD60 pKa = 3.77YY61 pKa = 9.96KK62 pKa = 11.1RR63 pKa = 11.84RR64 pKa = 11.84MADD67 pKa = 2.79AVSRR71 pKa = 11.84KK72 pKa = 10.22KK73 pKa = 10.07MDD75 pKa = 3.75TKK77 pKa = 10.32PTDD80 pKa = 3.46PTGIEE85 pKa = 4.1PDD87 pKa = 3.36DD88 pKa = 3.93HH89 pKa = 7.87LKK91 pKa = 10.48EE92 pKa = 4.18RR93 pKa = 11.84SSLRR97 pKa = 11.84YY98 pKa = 10.4GNVLDD103 pKa = 4.38VNAIDD108 pKa = 3.67IEE110 pKa = 4.45EE111 pKa = 4.59PSGQTADD118 pKa = 3.07WYY120 pKa = 9.87TIGVYY125 pKa = 10.42VIGFTLPIILKK136 pKa = 9.61ALYY139 pKa = 8.83MLSTRR144 pKa = 11.84GRR146 pKa = 11.84QTVKK150 pKa = 10.03EE151 pKa = 4.09NKK153 pKa = 7.61GTRR156 pKa = 11.84IRR158 pKa = 11.84FKK160 pKa = 10.94DD161 pKa = 3.35DD162 pKa = 2.88TSFEE166 pKa = 5.01DD167 pKa = 3.01INGIRR172 pKa = 11.84RR173 pKa = 11.84PKK175 pKa = 9.58HH176 pKa = 6.21LYY178 pKa = 10.21VSMPTAQSTMKK189 pKa = 10.72AEE191 pKa = 4.02EE192 pKa = 4.06LTPGRR197 pKa = 11.84FRR199 pKa = 11.84TIVCGLFPTQIQVRR213 pKa = 11.84NIMSPVMGVIGFSFFVKK230 pKa = 10.14DD231 pKa = 2.89WSEE234 pKa = 4.34RR235 pKa = 11.84IRR237 pKa = 11.84EE238 pKa = 4.09FMEE241 pKa = 4.24KK242 pKa = 9.51EE243 pKa = 4.07CPFIKK248 pKa = 10.5PEE250 pKa = 4.12VKK252 pKa = 10.17PGTPAQEE259 pKa = 3.72IEE261 pKa = 3.9MLKK264 pKa = 10.37RR265 pKa = 11.84NKK267 pKa = 9.79IYY269 pKa = 10.33FMQRR273 pKa = 11.84QDD275 pKa = 3.84VLDD278 pKa = 4.28KK279 pKa = 11.52NHH281 pKa = 6.16VADD284 pKa = 3.67IDD286 pKa = 3.88KK287 pKa = 11.09LIDD290 pKa = 3.44YY291 pKa = 7.82AASGDD296 pKa = 3.84PTSPDD301 pKa = 3.95NIDD304 pKa = 3.74SPNAPWVFACAPDD317 pKa = 3.86RR318 pKa = 11.84CPPTCIYY325 pKa = 9.3VAGMAEE331 pKa = 4.22LGAFFSILQDD341 pKa = 3.28MRR343 pKa = 11.84NTIMASKK350 pKa = 9.3TVGTAEE356 pKa = 4.09EE357 pKa = 4.16KK358 pKa = 10.68LKK360 pKa = 10.88KK361 pKa = 10.03KK362 pKa = 10.65SSFYY366 pKa = 10.41QSYY369 pKa = 10.61LRR371 pKa = 11.84RR372 pKa = 11.84TQSMGIQLDD381 pKa = 3.34QRR383 pKa = 11.84IILLFMLEE391 pKa = 4.08WGKK394 pKa = 11.05EE395 pKa = 4.19MVDD398 pKa = 3.77HH399 pKa = 6.53FHH401 pKa = 7.32LGDD404 pKa = 4.56DD405 pKa = 4.07MDD407 pKa = 4.87PEE409 pKa = 4.67LRR411 pKa = 11.84GLAQALIDD419 pKa = 3.78QKK421 pKa = 11.41VKK423 pKa = 10.55EE424 pKa = 4.47ISNQEE429 pKa = 3.84PLKK432 pKa = 10.76II433 pKa = 4.1

Molecular weight: 49.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C760|L_PUUMS RNA-directed RNA polymerase L OS=Puumala virus (strain Sotkamo/V-2969/81) OX=39002 GN=L PE=3 SV=1
MM1 pKa = 7.56NSNLLLPDD9 pKa = 3.41KK10 pKa = 10.1NLRR13 pKa = 11.84MQRR16 pKa = 11.84EE17 pKa = 4.07QWKK20 pKa = 6.72WTQMTLIKK28 pKa = 8.13THH30 pKa = 6.83CKK32 pKa = 9.75PGNKK36 pKa = 8.62QCQHH40 pKa = 5.5WRR42 pKa = 11.84TNSQTTRR49 pKa = 11.84EE50 pKa = 4.58GWQMLCPGKK59 pKa = 10.44KK60 pKa = 9.18WILNLLTRR68 pKa = 11.84LGLNLMTTSRR78 pKa = 11.84RR79 pKa = 11.84DD80 pKa = 3.2QALGMEE86 pKa = 4.59MSLMM90 pKa = 4.12

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3827

90

2156

956.8

108.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.775 ± 0.408

2.718 ± 1.066

5.592 ± 0.716

5.932 ± 0.582

4.599 ± 0.588

5.749 ± 0.658

2.09 ± 0.154

6.245 ± 0.361

6.768 ± 0.363

10.295 ± 0.618

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.535 ± 0.893

3.893 ± 0.399

3.946 ± 0.417

4.625 ± 0.517

4.599 ± 0.878

6.898 ± 0.631

6.323 ± 1.102

6.48 ± 0.724

1.516 ± 0.306

3.423 ± 0.55
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski