Beihai mantis shrimp virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFK8|A0A1L3KFK8_9VIRU RNA replicase OS=Beihai mantis shrimp virus 6 OX=1922433 PE=3 SV=1
MM1 pKa = 7.54VGQQSIGAAVTTVPVRR17 pKa = 11.84PPTNSIRR24 pKa = 11.84IRR26 pKa = 11.84GSDD29 pKa = 3.4TVTLIKK35 pKa = 10.6DD36 pKa = 3.34VATIGTEE43 pKa = 3.91AGDD46 pKa = 3.55VLIDD50 pKa = 3.7LRR52 pKa = 11.84LVPSISNRR60 pKa = 11.84LAIHH64 pKa = 5.62ATCYY68 pKa = 10.95NEE70 pKa = 4.11IFYY73 pKa = 10.59HH74 pKa = 5.54GLKK77 pKa = 10.56ARR79 pKa = 11.84INGQASSLTVGSIIMAFCSDD99 pKa = 4.18PADD102 pKa = 4.74EE103 pKa = 4.78IPSGVEE109 pKa = 3.56AIAWARR115 pKa = 11.84SQQCNVSGKK124 pKa = 8.11YY125 pKa = 8.59WEE127 pKa = 4.59TIEE130 pKa = 5.61LNIPHH135 pKa = 6.25SQLVGPNGGCFKK147 pKa = 11.11NNAGTSTAPRR157 pKa = 11.84TYY159 pKa = 10.66SPGFLAVIVVSPANQSTPLEE179 pKa = 4.38LQLEE183 pKa = 4.41WDD185 pKa = 3.65VTLRR189 pKa = 11.84NPTLNTLVEE198 pKa = 4.21EE199 pKa = 5.12GPRR202 pKa = 11.84VVTALKK208 pKa = 10.81DD209 pKa = 3.68FGLQGSATADD219 pKa = 3.24QAYY222 pKa = 10.44DD223 pKa = 3.58PNLKK227 pKa = 9.95VFPEE231 pKa = 4.55GDD233 pKa = 3.18LAATDD238 pKa = 4.59FSPSLEE244 pKa = 3.73ADD246 pKa = 3.13KK247 pKa = 11.29YY248 pKa = 9.22YY249 pKa = 10.53VIPGGQVTVTANTGASGAPASVVATHH275 pKa = 6.84IGLVGDD281 pKa = 3.4KK282 pKa = 10.74VGLYY286 pKa = 9.86RR287 pKa = 11.84YY288 pKa = 9.52LISSDD293 pKa = 3.17EE294 pKa = 4.35FKK296 pKa = 10.97AITATEE302 pKa = 4.18LPYY305 pKa = 10.72KK306 pKa = 10.12PSLLAAPTWRR316 pKa = 11.84EE317 pKa = 3.38ASEE320 pKa = 4.13WKK322 pKa = 9.29TDD324 pKa = 3.56PDD326 pKa = 3.8SPGFGTPTQGFRR338 pKa = 11.84LLSLQSSPSRR348 pKa = 11.84RR349 pKa = 11.84RR350 pKa = 11.84AA351 pKa = 3.04

Molecular weight: 37.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFH9|A0A1L3KFH9_9VIRU Capsid protein OS=Beihai mantis shrimp virus 6 OX=1922433 PE=3 SV=1
MM1 pKa = 7.71DD2 pKa = 4.44TLLQVLRR9 pKa = 11.84GIAPGAASLLRR20 pKa = 11.84LTRR23 pKa = 11.84WVAGNLKK30 pKa = 8.83STLLGVPWTTLPATTTSSLLATLRR54 pKa = 11.84WTIKK58 pKa = 10.44FLVQSKK64 pKa = 9.97EE65 pKa = 3.89RR66 pKa = 11.84LLPALTLTTTSLRR79 pKa = 11.84EE80 pKa = 4.05AGTGCLGEE88 pKa = 4.85EE89 pKa = 4.04IWEE92 pKa = 4.29SSTPSVLLQLPDD104 pKa = 3.7PTEE107 pKa = 4.22SALSPLKK114 pKa = 9.39TALWNTQFQVGRR126 pKa = 11.84FGDD129 pKa = 3.54IEE131 pKa = 4.39FGIGLLAEE139 pKa = 4.24SMSYY143 pKa = 8.13YY144 pKa = 9.9TNRR147 pKa = 11.84TISMVIHH154 pKa = 6.67PGCSTACDD162 pKa = 3.84RR163 pKa = 11.84SCSCWDD169 pKa = 3.48FRR171 pKa = 11.84PSPSLRR177 pKa = 11.84SIMPGLFLIWLTARR191 pKa = 11.84LCGLSPSIRR200 pKa = 11.84LGYY203 pKa = 8.29MAGYY207 pKa = 10.5LSRR210 pKa = 11.84CIFAGCGGWTTVAHH224 pKa = 6.73LALAGICIAPYY235 pKa = 7.8WTANLPLAA243 pKa = 4.76

Molecular weight: 26.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1748

121

1033

437.0

47.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.982 ± 0.718

2.002 ± 0.356

5.206 ± 0.838

5.092 ± 0.853

3.146 ± 0.397

8.238 ± 0.333

2.174 ± 0.559

3.776 ± 0.848

4.005 ± 0.59

10.069 ± 1.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.487 ± 0.263

2.574 ± 0.39

6.636 ± 0.221

3.432 ± 0.29

6.922 ± 0.763

7.037 ± 0.785

7.38 ± 0.807

6.808 ± 0.671

2.002 ± 0.369

3.032 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski