Aeromonas phage 4_4512

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9N4E9|A0A5B9N4E9_9CAUD Uncharacterized protein OS=Aeromonas phage 4_4512 OX=2588516 GN=44512_012 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 3.88KK3 pKa = 10.17MRR5 pKa = 11.84EE6 pKa = 3.99EE7 pKa = 4.17FEE9 pKa = 4.12AWCASIGFTLKK20 pKa = 10.66PNAFDD25 pKa = 3.73NGDD28 pKa = 3.27YY29 pKa = 11.51AMGYY33 pKa = 9.39GWEE36 pKa = 4.49EE37 pKa = 3.72WNAWQASRR45 pKa = 11.84AALCVQMPRR54 pKa = 11.84CCNEE58 pKa = 3.56EE59 pKa = 3.68QDD61 pKa = 4.09SYY63 pKa = 11.49RR64 pKa = 11.84LNIIDD69 pKa = 4.37SLDD72 pKa = 3.74DD73 pKa = 4.22AGVSYY78 pKa = 10.42EE79 pKa = 3.86

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N478|A0A5B9N478_9CAUD HTH cro/C1-type domain-containing protein OS=Aeromonas phage 4_4512 OX=2588516 GN=44512_051 PE=4 SV=1
MM1 pKa = 7.13TRR3 pKa = 11.84EE4 pKa = 3.62QALASAEE11 pKa = 3.92AQYY14 pKa = 10.3RR15 pKa = 11.84QHH17 pKa = 6.86RR18 pKa = 11.84ATYY21 pKa = 9.64VATLSPEE28 pKa = 3.74QLGAIQRR35 pKa = 11.84NAAKK39 pKa = 9.85PRR41 pKa = 11.84PTGRR45 pKa = 11.84RR46 pKa = 11.84ITIEE50 pKa = 3.82RR51 pKa = 11.84EE52 pKa = 3.56RR53 pKa = 11.84EE54 pKa = 3.89RR55 pKa = 11.84VVLMSQCPYY64 pKa = 10.96VGFATITSKK73 pKa = 11.28KK74 pKa = 10.17NGSLL78 pKa = 3.48

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12668

30

832

207.7

22.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.554 ± 0.37

1.318 ± 0.172

6.102 ± 0.254

6.173 ± 0.39

3.56 ± 0.217

8.052 ± 0.339

1.689 ± 0.188

5.857 ± 0.2

5.328 ± 0.341

7.554 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.094 ± 0.261

4.255 ± 0.235

4.097 ± 0.23

4.697 ± 0.408

5.549 ± 0.238

6.449 ± 0.272

5.194 ± 0.403

6.078 ± 0.244

1.666 ± 0.185

2.731 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski