Xanthomonas phage OP2
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2NPD0|Q2NPD0_9CAUD Uncharacterized protein OS=Xanthomonas phage OP2 OX=331627 PE=4 SV=1
MM1 pKa = 7.0 TAATLTPTEE10 pKa = 4.39 DD11 pKa = 3.33 AVFDD15 pKa = 4.02 AMFGFLAKK23 pKa = 10.67 VLDD26 pKa = 4.88 LPDD29 pKa = 3.85 DD30 pKa = 3.67 TQAIIKK36 pKa = 8.99 GFQNLSSTPTGSCVVVSPGMMTRR59 pKa = 11.84 QDD61 pKa = 3.6 FGSRR65 pKa = 11.84 LYY67 pKa = 11.01 DD68 pKa = 3.77 PGLSKK73 pKa = 10.96 VVIEE77 pKa = 4.07 AHH79 pKa = 5.49 LTYY82 pKa = 10.29 SYY84 pKa = 11.13 QVDD87 pKa = 4.34 CYY89 pKa = 11.44 GPLAPTWASVISVAWKK105 pKa = 10.82 SMWGVDD111 pKa = 3.29 NTAPAFAPLYY121 pKa = 10.83 ADD123 pKa = 5.5 APQQLNIVNSEE134 pKa = 4.09 GQFEE138 pKa = 4.28 QRR140 pKa = 11.84 FMVRR144 pKa = 11.84 LFGQVNQRR152 pKa = 11.84 VALPQDD158 pKa = 3.69 FFDD161 pKa = 4.58 SVQLTSLNIADD172 pKa = 5.19 LLPP175 pKa = 4.31
Molecular weight: 19.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.235
IPC2_protein 4.202
IPC_protein 4.164
Toseland 3.935
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.126
Rodwell 3.986
Grimsley 3.846
Solomon 4.151
Lehninger 4.113
Nozaki 4.279
DTASelect 4.558
Thurlkill 3.999
EMBOSS 4.126
Sillero 4.279
Patrickios 3.503
IPC_peptide 4.151
IPC2_peptide 4.253
IPC2.peptide.svr19 4.219
Protein with the highest isoelectric point:
>tr|Q2NP74|Q2NP74_9CAUD Uncharacterized protein OS=Xanthomonas phage OP2 OX=331627 PE=4 SV=1
MM1 pKa = 7.57 SRR3 pKa = 11.84 QTNSTTKK10 pKa = 10.33 RR11 pKa = 11.84 NPRR14 pKa = 11.84 DD15 pKa = 3.5 FLDD18 pKa = 4.41 ADD20 pKa = 3.84 ATPVPTWTVRR30 pKa = 11.84 EE31 pKa = 4.08 LVDD34 pKa = 3.54 AVGIEE39 pKa = 4.11 TAAQLWQISTNNARR53 pKa = 11.84 QVRR56 pKa = 11.84 HH57 pKa = 6.34 RR58 pKa = 11.84 GAASLEE64 pKa = 4.05 RR65 pKa = 11.84 MQVLHH70 pKa = 5.96 AHH72 pKa = 6.11 IRR74 pKa = 11.84 TNEE77 pKa = 3.48 LHH79 pKa = 5.86 YY80 pKa = 10.91 RR81 pKa = 11.84 EE82 pKa = 4.36 TLVTIYY88 pKa = 9.8 STGAFRR94 pKa = 11.84 RR95 pKa = 11.84 QSS97 pKa = 3.0
Molecular weight: 11.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.589
IPC_protein 10.833
Toseland 10.672
ProMoST 10.847
Dawson 10.789
Bjellqvist 10.672
Wikipedia 11.155
Rodwell 10.599
Grimsley 10.862
Solomon 11.082
Lehninger 11.008
Nozaki 10.657
DTASelect 10.672
Thurlkill 10.701
EMBOSS 11.125
Sillero 10.745
Patrickios 10.423
IPC_peptide 11.067
IPC2_peptide 9.926
IPC2.peptide.svr19 8.717
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
62
0
62
13949
60
837
225.0
24.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.488 ± 0.43
0.824 ± 0.102
6.065 ± 0.319
4.387 ± 0.323
2.911 ± 0.168
7.872 ± 0.496
1.57 ± 0.128
4.495 ± 0.187
3.004 ± 0.295
8.696 ± 0.344
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.38 ± 0.137
3.326 ± 0.192
5.606 ± 0.258
4.316 ± 0.232
6.667 ± 0.397
6.273 ± 0.276
7.14 ± 0.3
7.527 ± 0.324
1.541 ± 0.111
2.896 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here