Phalacrocorax carbo (Great cormorant) (Pelecanus carbo)
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8990 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A093PD19|A0A093PD19_PHACA UPF0556 protein C19orf10 (Fragment) OS=Phalacrocorax carbo OX=9209 GN=N336_02048 PE=4 SV=1
FF1 pKa = 7.63 LLPSRR6 pKa = 11.84 LSWLEE11 pKa = 3.41 NSGAGFSLDD20 pKa = 3.51 YY21 pKa = 9.25 PTISLHH27 pKa = 6.36 AVSRR31 pKa = 11.84 DD32 pKa = 3.07 LNAYY36 pKa = 7.9 PWEE39 pKa = 4.19 HH40 pKa = 6.86 LYY42 pKa = 11.74 VMVNAKK48 pKa = 10.3 FEE50 pKa = 4.48 EE51 pKa = 4.5 EE52 pKa = 4.09 TKK54 pKa = 9.69 EE55 pKa = 3.9 APMAEE60 pKa = 4.2 GEE62 pKa = 4.24 EE63 pKa = 4.28 EE64 pKa = 4.66 EE65 pKa = 4.91 EE66 pKa = 5.45 DD67 pKa = 3.82 SDD69 pKa = 6.17 DD70 pKa = 3.89 DD71 pKa = 4.15 VEE73 pKa = 5.15 PIAEE77 pKa = 4.09 FRR79 pKa = 11.84 FVPSDD84 pKa = 3.36 KK85 pKa = 10.88 SALEE89 pKa = 3.9 AMFSAMCEE97 pKa = 4.27 CQALHH102 pKa = 7.41 PDD104 pKa = 4.21 PDD106 pKa = 5.77 DD107 pKa = 4.05 EE108 pKa = 7.61 DD109 pKa = 4.46 SDD111 pKa = 3.76 NDD113 pKa = 3.97 YY114 pKa = 11.37 EE115 pKa = 4.59 GEE117 pKa = 4.16 EE118 pKa = 4.41 YY119 pKa = 10.56 DD120 pKa = 4.4 VEE122 pKa = 4.23 AHH124 pKa = 6.13 EE125 pKa = 5.43 LEE127 pKa = 4.47 QGDD130 pKa = 3.63 IPSFYY135 pKa = 9.93 TYY137 pKa = 11.03 EE138 pKa = 4.17 EE139 pKa = 4.58 GLSHH143 pKa = 6.9 LTAEE147 pKa = 4.71 GQATLEE153 pKa = 4.11 RR154 pKa = 11.84 LEE156 pKa = 4.74 GMLAQSVSSQYY167 pKa = 11.66 NMAGVRR173 pKa = 11.84 TEE175 pKa = 3.7 DD176 pKa = 3.58 SIRR179 pKa = 11.84 EE180 pKa = 4.1 FEE182 pKa = 5.37 DD183 pKa = 3.48 GMEE186 pKa = 3.92 VDD188 pKa = 3.78 IAPVVAGQFEE198 pKa = 4.42 DD199 pKa = 5.26 AEE201 pKa = 4.32 VDD203 pKa = 3.59 HH204 pKa = 7.08
Molecular weight: 22.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.795
IPC_protein 3.77
Toseland 3.579
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.605
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.834
DTASelect 3.986
Thurlkill 3.605
EMBOSS 3.63
Sillero 3.872
Patrickios 1.825
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|A0A093RGD1|A0A093RGD1_PHACA Protein WWC3 (Fragment) OS=Phalacrocorax carbo OX=9209 GN=N336_12111 PE=4 SV=1
MM1 pKa = 7.67 AAQSAPKK8 pKa = 10.16 VVLKK12 pKa = 10.04 STTKK16 pKa = 9.35 MSLNEE21 pKa = 4.25 RR22 pKa = 11.84 FTNMLKK28 pKa = 10.36 NKK30 pKa = 9.73 QPMPVNIRR38 pKa = 11.84 ATMQQQQQLASARR51 pKa = 11.84 NRR53 pKa = 11.84 RR54 pKa = 11.84 LAQQMEE60 pKa = 4.32 NRR62 pKa = 11.84 PSVQAALKK70 pKa = 10.37 LKK72 pKa = 10.39 QKK74 pKa = 9.42 SLKK77 pKa = 9.72 QRR79 pKa = 11.84 LGKK82 pKa = 10.77 SNIQARR88 pKa = 11.84 LGRR91 pKa = 11.84 PAGPLARR98 pKa = 11.84 GAMGGRR104 pKa = 11.84 GLSMGQRR111 pKa = 11.84 GLPRR115 pKa = 11.84 GAMRR119 pKa = 11.84 GGRR122 pKa = 11.84 GARR125 pKa = 11.84 ALLRR129 pKa = 11.84 GGVALRR135 pKa = 11.84 GRR137 pKa = 11.84 MGLRR141 pKa = 11.84 RR142 pKa = 11.84 GGLRR146 pKa = 11.84 GRR148 pKa = 11.84 GGPGRR153 pKa = 11.84 GGLGRR158 pKa = 11.84 GAMGRR163 pKa = 3.6
Molecular weight: 17.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.503
IPC2_protein 11.125
IPC_protein 12.72
Toseland 12.896
ProMoST 13.393
Dawson 12.896
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.544
Grimsley 12.925
Solomon 13.393
Lehninger 13.29
Nozaki 12.896
DTASelect 12.881
Thurlkill 12.896
EMBOSS 13.393
Sillero 12.896
Patrickios 12.252
IPC_peptide 13.393
IPC2_peptide 12.384
IPC2.peptide.svr19 9.141
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8990
0
8990
3797346
31
5869
422.4
47.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.278 ± 0.023
2.233 ± 0.023
5.083 ± 0.017
7.221 ± 0.027
3.93 ± 0.018
5.761 ± 0.033
2.548 ± 0.015
5.111 ± 0.02
6.544 ± 0.028
9.681 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.268 ± 0.01
4.244 ± 0.016
5.174 ± 0.031
4.683 ± 0.022
5.089 ± 0.019
8.245 ± 0.037
5.441 ± 0.018
6.26 ± 0.021
1.177 ± 0.008
3.017 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here