Acinetobacter sp. TGL-Y2
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3287 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A143G7K6|A0A143G7K6_9GAMM Uncharacterized protein OS=Acinetobacter sp. TGL-Y2 OX=1407071 GN=AMD27_15840 PE=4 SV=1
MM1 pKa = 7.3 FKK3 pKa = 10.61 KK4 pKa = 10.24 IVLATAIIASLGTAHH19 pKa = 8.12 AYY21 pKa = 7.95 QAEE24 pKa = 4.34 VGGTISLVDD33 pKa = 4.0 PDD35 pKa = 4.75 QGDD38 pKa = 3.6 TSAGFAVDD46 pKa = 3.24 GTYY49 pKa = 10.74 YY50 pKa = 10.73 FNPVQVKK57 pKa = 10.26 NNPLNEE63 pKa = 3.85 AAFLDD68 pKa = 3.96 RR69 pKa = 11.84 ASNVNGAISYY79 pKa = 10.3 IDD81 pKa = 4.04 YY82 pKa = 10.6 DD83 pKa = 4.22 LSDD86 pKa = 3.23 ITTFGAGVEE95 pKa = 4.25 YY96 pKa = 9.7 FVPNSDD102 pKa = 4.36 FYY104 pKa = 11.67 VSANIGQTQLGSDD117 pKa = 3.53 GSEE120 pKa = 4.21 DD121 pKa = 4.14 FDD123 pKa = 3.79 TTTYY127 pKa = 9.99 SAEE130 pKa = 4.15 VGYY133 pKa = 10.68 LPAPGLLVAVGLTGFDD149 pKa = 3.53 NDD151 pKa = 5.1 LGDD154 pKa = 5.48 DD155 pKa = 3.66 VDD157 pKa = 3.67 PTLRR161 pKa = 11.84 AKK163 pKa = 10.61 YY164 pKa = 7.88 VTQVGAYY171 pKa = 10.29 DD172 pKa = 3.65 MNFEE176 pKa = 4.13 GAATFGDD183 pKa = 3.45 INAYY187 pKa = 10.15 GLGADD192 pKa = 4.81 LYY194 pKa = 10.8 LDD196 pKa = 3.4 KK197 pKa = 10.74 TFSVGAGFSGSDD209 pKa = 3.4 ADD211 pKa = 5.42 GVDD214 pKa = 3.53 DD215 pKa = 5.08 DD216 pKa = 4.9 VFNIRR221 pKa = 11.84 AKK223 pKa = 10.73 KK224 pKa = 10.33 FFTQQISLEE233 pKa = 4.15 GSVNFADD240 pKa = 3.89 AGNVFGVRR248 pKa = 11.84 GAYY251 pKa = 9.79 RR252 pKa = 11.84 FF253 pKa = 3.58
Molecular weight: 26.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.732
IPC_protein 3.757
Toseland 3.528
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.19
Thurlkill 3.592
EMBOSS 3.757
Sillero 3.884
Patrickios 1.138
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|A0A143G7V6|A0A143G7V6_9GAMM Uncharacterized protein OS=Acinetobacter sp. TGL-Y2 OX=1407071 GN=AMD27_13585 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.24 GRR39 pKa = 11.84 QSLTVV44 pKa = 3.17
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3287
0
3287
989157
33
4289
300.9
33.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.46 ± 0.086
0.94 ± 0.015
5.41 ± 0.048
5.857 ± 0.048
4.3 ± 0.038
6.37 ± 0.06
2.35 ± 0.033
7.029 ± 0.04
5.942 ± 0.047
10.227 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.515 ± 0.03
4.77 ± 0.053
3.773 ± 0.036
5.173 ± 0.043
4.147 ± 0.044
6.397 ± 0.043
5.375 ± 0.066
6.6 ± 0.046
1.173 ± 0.021
3.191 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here