Human adenovirus D serotype 8 (HAdV-8) (Human adenovirus 8)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Rowavirales; Adenoviridae; Mastadenovirus; Human mastadenovirus D

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9A5A8|B9A5A8_ADE08 E1B 55 kDa protein OS=Human adenovirus D serotype 8 OX=31545 GN=E1B PE=3 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84HH3 pKa = 5.55LHH5 pKa = 6.27LLSSTVPIDD14 pKa = 3.35MAALLLEE21 pKa = 5.44DD22 pKa = 4.0YY23 pKa = 11.4VNTILEE29 pKa = 4.76DD30 pKa = 3.9EE31 pKa = 4.51LHH33 pKa = 6.65LSPFEE38 pKa = 5.02LGPTLQDD45 pKa = 4.0LYY47 pKa = 11.2DD48 pKa = 4.47LEE50 pKa = 5.32VNAQEE55 pKa = 4.25NDD57 pKa = 3.71PNEE60 pKa = 4.07EE61 pKa = 3.9AVNLIFPEE69 pKa = 4.24SMILQADD76 pKa = 3.86IASEE80 pKa = 4.07AVPTSVYY87 pKa = 10.51TPTLPPIPKK96 pKa = 10.04LEE98 pKa = 4.35EE99 pKa = 3.75EE100 pKa = 4.71DD101 pKa = 4.8KK102 pKa = 11.51LDD104 pKa = 3.62LRR106 pKa = 11.84CYY108 pKa = 10.69EE109 pKa = 4.2EE110 pKa = 5.23GFPPSDD116 pKa = 3.74SEE118 pKa = 4.84DD119 pKa = 3.54EE120 pKa = 4.33RR121 pKa = 11.84GEE123 pKa = 3.71QSMAIISDD131 pKa = 3.88YY132 pKa = 11.09ACVVVEE138 pKa = 3.89NHH140 pKa = 6.02FVLDD144 pKa = 3.78NPEE147 pKa = 4.04VPGQGCKK154 pKa = 9.18SCQYY158 pKa = 10.37HH159 pKa = 6.64RR160 pKa = 11.84EE161 pKa = 4.04QTGDD165 pKa = 3.67PNASCALCYY174 pKa = 10.26MKK176 pKa = 10.28MSFSFIYY183 pKa = 10.61SPVSEE188 pKa = 5.13DD189 pKa = 3.08EE190 pKa = 4.27SSPLEE195 pKa = 4.22EE196 pKa = 4.93DD197 pKa = 3.85HH198 pKa = 7.35PSPPDD203 pKa = 3.46LTNDD207 pKa = 2.89TPLQVRR213 pKa = 11.84RR214 pKa = 11.84PTPVRR219 pKa = 11.84PSGEE223 pKa = 3.6RR224 pKa = 11.84RR225 pKa = 11.84AAVYY229 pKa = 10.16KK230 pKa = 10.56IEE232 pKa = 6.07DD233 pKa = 3.87LLQDD237 pKa = 3.43VGGNEE242 pKa = 4.23PLNLSLKK249 pKa = 10.23RR250 pKa = 11.84PRR252 pKa = 11.84NN253 pKa = 3.53

Molecular weight: 28.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9A5B9|B9A5B9_ADE08 Isoform of Q3S8C3 Pre-protein VI OS=Human adenovirus D serotype 8 OX=31545 GN=L3 PE=1 SV=1
MM1 pKa = 7.77ALTCRR6 pKa = 11.84LRR8 pKa = 11.84IPVPNYY14 pKa = 8.76RR15 pKa = 11.84GRR17 pKa = 11.84SRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GMAGSGLNRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AMRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84LSGGFLPALIPIIAAAIGTIPGIASVALQASQRR73 pKa = 11.84RR74 pKa = 3.83

Molecular weight: 8.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

22

36

11001

65

1091

305.6

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.008 ± 0.398

2.027 ± 0.294

5.009 ± 0.295

5.899 ± 0.46

3.881 ± 0.272

6.154 ± 0.289

2.482 ± 0.238

4.118 ± 0.26

4.3 ± 0.426

9.772 ± 0.439

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.192

4.809 ± 0.392

6.499 ± 0.301

4.491 ± 0.25

7.236 ± 0.595

6.363 ± 0.277

6.018 ± 0.416

6.472 ± 0.294

1.327 ± 0.124

3.409 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski