Neisseria chenwenguii
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2219 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220S3H5|A0A220S3H5_9NEIS Uncharacterized protein OS=Neisseria chenwenguii OX=1853278 GN=BG910_10200 PE=4 SV=1
MM1 pKa = 7.05 KK2 pKa = 9.54 TLPFQAEE9 pKa = 3.63 IAEE12 pKa = 4.08 RR13 pKa = 11.84 MLVNTEE19 pKa = 3.92 NEE21 pKa = 4.57 SIHH24 pKa = 6.36 PEE26 pKa = 3.71 AVFVEE31 pKa = 4.6 TANGYY36 pKa = 8.55 WIAWHH41 pKa = 7.25 DD42 pKa = 3.75 GTVALLAPDD51 pKa = 4.5 TPPDD55 pKa = 3.65 IPCFWVEE62 pKa = 3.95 GAEE65 pKa = 4.23 SLEE68 pKa = 3.88 EE69 pKa = 3.68 LAAMVEE75 pKa = 4.0 NGEE78 pKa = 3.94 FDD80 pKa = 3.45 EE81 pKa = 5.09 VEE83 pKa = 4.39 EE84 pKa = 5.19 FDD86 pKa = 5.14 GDD88 pKa = 3.62 DD89 pKa = 5.09 DD90 pKa = 4.02 AWQEE94 pKa = 3.64 AAAGCGHH101 pKa = 6.15 NHH103 pKa = 7.57 DD104 pKa = 4.91 DD105 pKa = 4.71 ACGCGHH111 pKa = 7.65
Molecular weight: 12.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.567
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.579
Grimsley 3.478
Solomon 3.694
Lehninger 3.656
Nozaki 3.834
DTASelect 3.973
Thurlkill 3.605
EMBOSS 3.63
Sillero 3.859
Patrickios 0.604
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.756
Protein with the highest isoelectric point:
>tr|A0A220S0V9|A0A220S0V9_9NEIS 50S ribosomal protein L16 OS=Neisseria chenwenguii OX=1853278 GN=rplP PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSVTKK11 pKa = 10.56 RR12 pKa = 11.84 KK13 pKa = 7.91 RR14 pKa = 11.84 THH16 pKa = 5.89 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2219
0
2219
658136
27
3738
296.6
32.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.072 ± 0.088
0.98 ± 0.02
5.249 ± 0.048
5.981 ± 0.054
4.121 ± 0.045
7.677 ± 0.062
2.088 ± 0.027
5.368 ± 0.046
5.295 ± 0.046
10.068 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.554 ± 0.031
3.993 ± 0.052
4.227 ± 0.035
4.102 ± 0.037
5.333 ± 0.052
5.434 ± 0.039
5.261 ± 0.05
7.071 ± 0.047
1.255 ± 0.022
2.872 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here