Wenling crustacean virus 12

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Nyamiviridae; Crustavirus; Wenling crustavirus

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN88|A0A1L3KN88_9MONO Large structural protein OS=Wenling crustacean virus 12 OX=1923481 PE=4 SV=1
MM1 pKa = 8.15PDD3 pKa = 2.94TRR5 pKa = 11.84SGARR9 pKa = 11.84HH10 pKa = 6.44GAQQDD15 pKa = 4.03PLPGTSNQPPPEE27 pKa = 3.91QGHH30 pKa = 6.89RR31 pKa = 11.84YY32 pKa = 6.35TPYY35 pKa = 10.12YY36 pKa = 8.54IPRR39 pKa = 11.84RR40 pKa = 11.84AKK42 pKa = 10.35HH43 pKa = 5.68KK44 pKa = 10.52SQTISQIPAAKK55 pKa = 9.82RR56 pKa = 11.84EE57 pKa = 4.1PIKK60 pKa = 10.4RR61 pKa = 11.84YY62 pKa = 9.9RR63 pKa = 11.84KK64 pKa = 8.02ATAAAMLLDD73 pKa = 3.21IHH75 pKa = 6.95APKK78 pKa = 10.48DD79 pKa = 3.41YY80 pKa = 9.93TLYY83 pKa = 9.85ISCMGYY89 pKa = 10.74LSGLSSEE96 pKa = 4.57VMEE99 pKa = 5.04QLFEE103 pKa = 4.61HH104 pKa = 7.08KK105 pKa = 10.35DD106 pKa = 3.66GLIVTHH112 pKa = 7.26PSTTNPPPGITPEE125 pKa = 3.99VAMSAVVIAGYY136 pKa = 8.63ATVTNLAKK144 pKa = 10.55EE145 pKa = 3.97ITTDD149 pKa = 2.82NVAYY153 pKa = 9.52VGRR156 pKa = 11.84RR157 pKa = 11.84INAIGADD164 pKa = 3.71AEE166 pKa = 4.28NDD168 pKa = 3.47YY169 pKa = 9.57LTNVGDD175 pKa = 4.12SVVRR179 pKa = 11.84SYY181 pKa = 11.96LDD183 pKa = 3.31LPAIRR188 pKa = 11.84DD189 pKa = 3.67ARR191 pKa = 11.84CFIVVRR197 pKa = 11.84EE198 pKa = 4.01AAQTIIEE205 pKa = 4.65DD206 pKa = 3.98LLVPTDD212 pKa = 3.47EE213 pKa = 5.57ASYY216 pKa = 11.33LDD218 pKa = 3.51TALYY222 pKa = 10.56KK223 pKa = 10.7SFEE226 pKa = 4.09LVFRR230 pKa = 11.84GFGMTALMEE239 pKa = 4.26MEE241 pKa = 4.65YY242 pKa = 9.03FTSIVTKK249 pKa = 10.67ALLLDD254 pKa = 3.82VVRR257 pKa = 11.84DD258 pKa = 3.3EE259 pKa = 4.54CKK261 pKa = 10.25RR262 pKa = 11.84FKK264 pKa = 10.7EE265 pKa = 3.77AFKK268 pKa = 10.42RR269 pKa = 11.84FKK271 pKa = 10.41SAQPAWDD278 pKa = 4.09LGRR281 pKa = 11.84LMKK284 pKa = 10.52LDD286 pKa = 5.13GINEE290 pKa = 4.24LNHH293 pKa = 6.59ANYY296 pKa = 9.03PHH298 pKa = 7.57LYY300 pKa = 7.92HH301 pKa = 7.0CAMARR306 pKa = 11.84VTAGSTFKK314 pKa = 10.84NYY316 pKa = 9.69QKK318 pKa = 10.7SDD320 pKa = 3.1HH321 pKa = 5.61QLRR324 pKa = 11.84EE325 pKa = 3.94SAATLTQFANTNLTMSYY342 pKa = 10.65GITAEE347 pKa = 3.91IANEE351 pKa = 3.61IHH353 pKa = 6.45QLTGKK358 pKa = 9.95DD359 pKa = 3.56VNSLIGTNLL368 pKa = 3.11

Molecular weight: 40.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNC6|A0A1L3KNC6_9MONO Uncharacterized protein OS=Wenling crustacean virus 12 OX=1923481 PE=4 SV=1
MM1 pKa = 7.77ASQHH5 pKa = 6.07DD6 pKa = 4.36TLSIHH11 pKa = 7.05ANQEE15 pKa = 4.04SLLQGPDD22 pKa = 3.15VSSEE26 pKa = 4.07EE27 pKa = 4.36ASTPPAPRR35 pKa = 11.84KK36 pKa = 9.82RR37 pKa = 11.84INIRR41 pKa = 11.84KK42 pKa = 9.46ASSQPSPHH50 pKa = 6.57KK51 pKa = 10.26RR52 pKa = 11.84PKK54 pKa = 10.63LGVTLEE60 pKa = 4.27SLSQQVKK67 pKa = 9.79IGFNKK72 pKa = 10.47LEE74 pKa = 4.01NQINSILTLLQTYY87 pKa = 7.23EE88 pKa = 4.06SRR90 pKa = 11.84VTEE93 pKa = 4.33LEE95 pKa = 3.95RR96 pKa = 11.84KK97 pKa = 8.07LASMGVNAGTTGPTLAKK114 pKa = 9.04QHH116 pKa = 5.92AVAPVTAPLLLPSTPVDD133 pKa = 3.23WDD135 pKa = 3.58NLL137 pKa = 3.84

Molecular weight: 14.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2277

137

1772

759.0

84.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.51 ± 1.115

1.669 ± 0.594

4.963 ± 0.61

5.578 ± 0.084

2.196 ± 0.468

5.973 ± 0.564

2.679 ± 0.144

6.192 ± 0.579

4.919 ± 0.281

10.716 ± 0.764

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.309

3.777 ± 0.495

5.709 ± 0.919

3.689 ± 0.874

5.665 ± 0.392

6.895 ± 1.215

7.071 ± 0.528

6.368 ± 0.394

1.361 ± 0.463

4.699 ± 1.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski