Streptococcus satellite phage Javan313

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZPF8|A0A4D5ZPF8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan313 OX=2558629 GN=JavanS313_0010 PE=4 SV=1
MM1 pKa = 7.51LRR3 pKa = 11.84NNLAKK8 pKa = 10.83LMIDD12 pKa = 3.64RR13 pKa = 11.84GISATQLFNDD23 pKa = 3.78TGIARR28 pKa = 11.84STISKK33 pKa = 9.86ISNNNTDD40 pKa = 4.44KK41 pKa = 11.27INLQTIDD48 pKa = 4.24KK49 pKa = 9.05ICNYY53 pKa = 10.38LEE55 pKa = 3.99VSPSEE60 pKa = 5.55FFDD63 pKa = 3.58FWPYY67 pKa = 10.63DD68 pKa = 3.5VKK70 pKa = 11.14VQCGFINFDD79 pKa = 3.2SLAEE83 pKa = 4.15VKK85 pKa = 10.01EE86 pKa = 4.02KK87 pKa = 10.28WKK89 pKa = 10.61AIPDD93 pKa = 3.81FKK95 pKa = 11.25EE96 pKa = 3.71PAFLLIEE103 pKa = 4.16FTRR106 pKa = 11.84GKK108 pKa = 9.96NIKK111 pKa = 9.94KK112 pKa = 8.86ILEE115 pKa = 3.75YY116 pKa = 9.9RR117 pKa = 11.84FNYY120 pKa = 9.05IEE122 pKa = 4.48EE123 pKa = 4.39YY124 pKa = 10.76EE125 pKa = 4.14PGIPFNNGYY134 pKa = 10.12LDD136 pKa = 5.31SIVLDD141 pKa = 3.77NSPSEE146 pKa = 3.98TSIFDD151 pKa = 3.47NMPVQFQNEE160 pKa = 4.01LVEE163 pKa = 4.24EE164 pKa = 4.39VKK166 pKa = 10.82EE167 pKa = 4.04KK168 pKa = 10.81LSEE171 pKa = 4.19TFEE174 pKa = 4.22VLPFSPTIEE183 pKa = 4.08NLDD186 pKa = 3.86LFTLSGLFF194 pKa = 3.55

Molecular weight: 22.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZJR1|A0A4D5ZJR1_9VIRU KilA protein OS=Streptococcus satellite phage Javan313 OX=2558629 GN=JavanS313_0012 PE=4 SV=1
MM1 pKa = 7.64FDD3 pKa = 4.35GFPEE7 pKa = 4.13RR8 pKa = 11.84PRR10 pKa = 11.84YY11 pKa = 8.12MKK13 pKa = 9.81RR14 pKa = 11.84DD15 pKa = 3.77RR16 pKa = 11.84YY17 pKa = 9.2HH18 pKa = 5.94KK19 pKa = 10.09HH20 pKa = 4.58YY21 pKa = 10.97KK22 pKa = 10.24KK23 pKa = 10.13FLKK26 pKa = 10.6YY27 pKa = 9.68IEE29 pKa = 4.58KK30 pKa = 10.6GDD32 pKa = 3.91RR33 pKa = 11.84FWLNGSRR40 pKa = 11.84LL41 pKa = 3.57

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

3015

41

492

177.4

20.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.771 ± 0.395

0.63 ± 0.129

5.373 ± 0.319

8.624 ± 0.627

5.406 ± 0.375

4.511 ± 0.295

1.924 ± 0.217

6.833 ± 0.468

9.221 ± 0.509

9.42 ± 0.512

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.342

5.439 ± 0.473

3.317 ± 0.589

3.98 ± 0.387

4.677 ± 0.37

5.307 ± 0.361

6.501 ± 0.256

5.506 ± 0.368

0.796 ± 0.181

4.245 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski