Crateriforma conspicua
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5367 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C5YEU5|A0A5C5YEU5_9PLAN Uncharacterized protein OS=Crateriforma conspicua OX=2527996 GN=Pan14r_40780 PE=4 SV=1
MM1 pKa = 7.68 IGNFLQVTPQQLQEE15 pKa = 4.84 LIASPEE21 pKa = 4.22 TIDD24 pKa = 4.86 DD25 pKa = 4.03 LLYY28 pKa = 10.59 PEE30 pKa = 5.9 DD31 pKa = 4.54 EE32 pKa = 5.04 DD33 pKa = 5.27 SGIDD37 pKa = 4.66 IDD39 pKa = 4.38 KK40 pKa = 9.82 AWHH43 pKa = 6.77 GIHH46 pKa = 6.78 FMLTGEE52 pKa = 4.63 QYY54 pKa = 11.19 DD55 pKa = 3.97 GTPPFSDD62 pKa = 3.99 VIFATSTVGDD72 pKa = 3.77 DD73 pKa = 3.5 VGYY76 pKa = 10.96 GPARR80 pKa = 11.84 YY81 pKa = 7.96 LTAPEE86 pKa = 4.06 VSAVADD92 pKa = 3.84 ALRR95 pKa = 11.84 EE96 pKa = 4.05 VPRR99 pKa = 11.84 DD100 pKa = 3.9 DD101 pKa = 3.33 FAKK104 pKa = 10.45 RR105 pKa = 11.84 YY106 pKa = 9.13 DD107 pKa = 3.8 AAALTANDD115 pKa = 4.69 IYY117 pKa = 10.44 PQIWSGDD124 pKa = 3.28 EE125 pKa = 3.92 DD126 pKa = 5.08 LDD128 pKa = 4.96 YY129 pKa = 11.54 LLSWYY134 pKa = 7.81 DD135 pKa = 3.48 TLRR138 pKa = 11.84 DD139 pKa = 3.87 YY140 pKa = 11.78 YY141 pKa = 11.3 LDD143 pKa = 3.92 AASKK147 pKa = 10.07 GNAMLQYY154 pKa = 11.21 LNN156 pKa = 4.37
Molecular weight: 17.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.49
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.846
Patrickios 0.744
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A5C5XS66|A0A5C5XS66_9PLAN DUF1080 domain-containing protein OS=Crateriforma conspicua OX=2527996 GN=Pan14r_50440 PE=4 SV=1
MM1 pKa = 7.59 AKK3 pKa = 9.95 KK4 pKa = 10.08 KK5 pKa = 9.75 AAKK8 pKa = 9.5 KK9 pKa = 9.58 RR10 pKa = 11.84 AVKK13 pKa = 10.28 KK14 pKa = 10.41 RR15 pKa = 11.84 ATKK18 pKa = 10.01 KK19 pKa = 9.98 AAVKK23 pKa = 10.48 KK24 pKa = 10.3 KK25 pKa = 8.73 VAKK28 pKa = 10.21 KK29 pKa = 9.87 RR30 pKa = 11.84 ATKK33 pKa = 10.5 KK34 pKa = 10.32 KK35 pKa = 8.75 VAKK38 pKa = 10.16 KK39 pKa = 9.87 KK40 pKa = 9.21 AAKK43 pKa = 9.82 KK44 pKa = 9.86 RR45 pKa = 11.84 ATKK48 pKa = 10.51 KK49 pKa = 10.28 KK50 pKa = 8.59 VAKK53 pKa = 10.28 KK54 pKa = 8.89 KK55 pKa = 7.12 TAKK58 pKa = 9.99 KK59 pKa = 10.12 RR60 pKa = 11.84 ATKK63 pKa = 10.28 KK64 pKa = 9.87 KK65 pKa = 8.97 AAKK68 pKa = 9.59 KK69 pKa = 10.04 RR70 pKa = 11.84 RR71 pKa = 3.58
Molecular weight: 8.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.228
IPC_protein 12.618
Toseland 12.852
ProMoST 13.29
Dawson 12.866
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 13.115
Grimsley 12.896
Solomon 13.305
Lehninger 13.217
Nozaki 12.852
DTASelect 12.808
Thurlkill 12.852
EMBOSS 13.334
Sillero 12.852
Patrickios 12.822
IPC_peptide 13.305
IPC2_peptide 12.266
IPC2.peptide.svr19 8.96
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5367
0
5367
2083014
29
12236
388.1
42.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.7 ± 0.032
1.17 ± 0.019
7.243 ± 0.045
5.363 ± 0.03
3.606 ± 0.02
7.913 ± 0.048
2.238 ± 0.023
5.023 ± 0.024
3.126 ± 0.035
9.168 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.337 ± 0.024
3.062 ± 0.029
5.325 ± 0.029
4.25 ± 0.024
7.004 ± 0.049
6.514 ± 0.028
5.701 ± 0.052
7.38 ± 0.031
1.556 ± 0.019
2.323 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here