SAR202 cluster bacterium AC-409-J13_OGT_754m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YH94|A0A5N8YH94_9CHLR 4-hydroxy-tetrahydrodipicolinate reductase OS=SAR202 cluster bacterium AC-409-J13_OGT_754m OX=2587834 GN=dapB PE=3 SV=1
MM1 pKa = 6.94QVSEE5 pKa = 4.42SALEE9 pKa = 4.12KK10 pKa = 10.31IKK12 pKa = 10.78EE13 pKa = 4.05VLEE16 pKa = 4.57DD17 pKa = 3.52EE18 pKa = 4.66GKK20 pKa = 10.55KK21 pKa = 10.41NSPLRR26 pKa = 11.84VIALPQPNGSVQYY39 pKa = 9.23MLTMEE44 pKa = 4.86QEE46 pKa = 4.36TQSDD50 pKa = 4.95DD51 pKa = 3.13ITIDD55 pKa = 3.27EE56 pKa = 5.46DD57 pKa = 4.19GVQFIVDD64 pKa = 3.57SDD66 pKa = 3.95SAPFLEE72 pKa = 4.97KK73 pKa = 10.23ATIDD77 pKa = 3.69FVEE80 pKa = 4.7DD81 pKa = 3.54LEE83 pKa = 4.79KK84 pKa = 11.24VGFTITNPEE93 pKa = 3.75YY94 pKa = 9.98PAASGCGSGGCGCGGGGCGCGGGGGGCGCGGG125 pKa = 3.39

Molecular weight: 12.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YH65|A0A5N8YH65_9CHLR Uncharacterized protein OS=SAR202 cluster bacterium AC-409-J13_OGT_754m OX=2587834 GN=FIM04_02720 PE=4 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.92RR4 pKa = 11.84TYY6 pKa = 9.93QPNVRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 8.74RR15 pKa = 11.84VHH17 pKa = 5.57GFFRR21 pKa = 11.84RR22 pKa = 11.84MLSKK26 pKa = 10.69GGRR29 pKa = 11.84LVLRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84LKK38 pKa = 10.07GRR40 pKa = 11.84HH41 pKa = 5.6RR42 pKa = 11.84LTVV45 pKa = 3.07

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

976

0

976

306694

32

1512

314.2

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.303 ± 0.088

0.972 ± 0.025

5.438 ± 0.059

6.093 ± 0.077

3.899 ± 0.055

7.947 ± 0.071

2.067 ± 0.034

7.761 ± 0.078

4.891 ± 0.063

9.904 ± 0.092

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.035

4.295 ± 0.053

4.427 ± 0.048

3.264 ± 0.045

5.096 ± 0.056

7.171 ± 0.054

5.595 ± 0.066

7.091 ± 0.067

1.236 ± 0.031

2.848 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski