Rice dwarf virus (isolate Fujian) (RDV)
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q98632|P2_RDVF Minor outer capsid protein P2 OS=Rice dwarf virus (isolate Fujian) OX=142804 PE=1 SV=1
MM1 pKa = 8.06 AYY3 pKa = 9.78 PNDD6 pKa = 3.48 VRR8 pKa = 11.84 NVWDD12 pKa = 3.78 VYY14 pKa = 11.35 NVFRR18 pKa = 11.84 DD19 pKa = 3.82 VPNRR23 pKa = 11.84 EE24 pKa = 3.59 HH25 pKa = 7.84 LIRR28 pKa = 11.84 DD29 pKa = 3.22 IRR31 pKa = 11.84 NGLVTVRR38 pKa = 11.84 NLTNMLTNMEE48 pKa = 4.97 RR49 pKa = 11.84 DD50 pKa = 3.62 DD51 pKa = 3.95 QLIIAQLSNMMKK63 pKa = 9.98 SLSIGIEE70 pKa = 3.81 KK71 pKa = 10.07 AQNEE75 pKa = 4.15 LSKK78 pKa = 11.26 LKK80 pKa = 8.63 TTDD83 pKa = 3.04 ADD85 pKa = 4.12 RR86 pKa = 11.84 AAVLADD92 pKa = 3.5 YY93 pKa = 7.51 QTSVLNIEE101 pKa = 4.58 RR102 pKa = 11.84 NTMLLTGYY110 pKa = 9.84 FKK112 pKa = 10.82 QLVLDD117 pKa = 3.58 LTGYY121 pKa = 10.14 VGASVYY127 pKa = 10.53 PILPFMITGDD137 pKa = 3.39 QSMMVDD143 pKa = 4.15 SINVNMKK150 pKa = 10.24 NVFDD154 pKa = 5.12 DD155 pKa = 3.32 KK156 pKa = 11.34 HH157 pKa = 5.42 EE158 pKa = 4.03 QEE160 pKa = 4.58 IVLPIHH166 pKa = 6.21 PACFVSTITEE176 pKa = 4.12 DD177 pKa = 3.39 TSSVVYY183 pKa = 10.89 ADD185 pKa = 3.47 GDD187 pKa = 3.69 EE188 pKa = 4.68 LYY190 pKa = 10.37 SVHH193 pKa = 6.11 VRR195 pKa = 11.84 HH196 pKa = 6.55 EE197 pKa = 4.05 NMTMYY202 pKa = 10.86 VNVLGEE208 pKa = 4.35 TVEE211 pKa = 4.25 TRR213 pKa = 11.84 QLSMIGEE220 pKa = 4.59 SIVPDD225 pKa = 3.91 DD226 pKa = 4.5 FAPSLLILRR235 pKa = 11.84 FSQDD239 pKa = 2.68 SVGEE243 pKa = 4.14 VFYY246 pKa = 11.0 LSHH249 pKa = 7.75 DD250 pKa = 4.18 NIKK253 pKa = 10.69 KK254 pKa = 10.09 FLGHH258 pKa = 5.74 SLEE261 pKa = 4.22 YY262 pKa = 8.67 TDD264 pKa = 4.92 KK265 pKa = 11.39 YY266 pKa = 11.25 VIFDD270 pKa = 3.32 VARR273 pKa = 11.84 RR274 pKa = 11.84 ASTTRR279 pKa = 11.84 NTITDD284 pKa = 4.27 GFCSVDD290 pKa = 3.32 GVPYY294 pKa = 10.62 LDD296 pKa = 4.4 GRR298 pKa = 11.84 FIYY301 pKa = 10.25 QPSGISADD309 pKa = 3.82 SNICAIYY316 pKa = 10.26 NSYY319 pKa = 10.73 VLDD322 pKa = 3.41 VLRR325 pKa = 11.84 YY326 pKa = 7.78 ITEE329 pKa = 4.31 CEE331 pKa = 3.69 VDD333 pKa = 3.73 TLRR336 pKa = 11.84 SVYY339 pKa = 10.55 DD340 pKa = 3.59 RR341 pKa = 11.84 TSSTVFSKK349 pKa = 10.18 TDD351 pKa = 3.16 VLRR354 pKa = 11.84 PRR356 pKa = 11.84 LLTMQSNISALSAATPQLANDD377 pKa = 4.76 VITSDD382 pKa = 3.73 STDD385 pKa = 3.75 LLSLGTVLTVSNEE398 pKa = 3.64 FTADD402 pKa = 3.48 DD403 pKa = 4.06 TTLSTSLAGHH413 pKa = 5.89 CQVDD417 pKa = 3.95 YY418 pKa = 11.68 SEE420 pKa = 5.98 GSPQDD425 pKa = 3.09 KK426 pKa = 10.82 SMSIPVSCDD435 pKa = 2.78 SSQLASSTVHH445 pKa = 6.47 SYY447 pKa = 10.83 SADD450 pKa = 3.24 ILGHH454 pKa = 5.18 GLKK457 pKa = 10.35 GDD459 pKa = 3.77 RR460 pKa = 11.84 NLNLMINVPGLMNPQKK476 pKa = 9.82 VTVDD480 pKa = 3.32 YY481 pKa = 10.95 VYY483 pKa = 11.53 SDD485 pKa = 3.74 GYY487 pKa = 10.55 KK488 pKa = 10.66 LNFASVVAPDD498 pKa = 3.21 APFWINATLQLSLSPSAHH516 pKa = 5.67 NMLSKK521 pKa = 9.92 LTPLDD526 pKa = 3.66 NDD528 pKa = 3.62 ACPGLKK534 pKa = 10.06 AQANTPVLVSMTINLDD550 pKa = 3.46 DD551 pKa = 4.18 ATPALGGEE559 pKa = 4.59 VIQNCVFKK567 pKa = 10.46 IHH569 pKa = 6.9 HH570 pKa = 6.82 GDD572 pKa = 3.39 DD573 pKa = 3.57 VYY575 pKa = 12.0 SFVTDD580 pKa = 4.06 FDD582 pKa = 4.76 VISYY586 pKa = 8.94 TSTSGTNCLKK596 pKa = 10.73 LISSVDD602 pKa = 3.1 ITSQLPSDD610 pKa = 3.15 MWIYY614 pKa = 11.16 VMNGSPDD621 pKa = 3.21 AAFISGDD628 pKa = 3.91 SVNMSSVDD636 pKa = 3.1 WHH638 pKa = 6.24 QSTSQTVGNYY648 pKa = 8.83 VYY650 pKa = 10.54 ATMKK654 pKa = 10.48 AYY656 pKa = 9.75 WNVTSYY662 pKa = 11.27 DD663 pKa = 3.5 VEE665 pKa = 3.95 ARR667 pKa = 11.84 PYY669 pKa = 8.52 ATYY672 pKa = 10.98 VPGKK676 pKa = 10.31 INFTAIDD683 pKa = 3.74 HH684 pKa = 6.99 ADD686 pKa = 3.68 VFVDD690 pKa = 4.98 DD691 pKa = 4.87 YY692 pKa = 10.77 NTGVNSYY699 pKa = 10.62 VIVNSRR705 pKa = 11.84 IYY707 pKa = 10.86 YY708 pKa = 9.63 KK709 pKa = 10.7 GNSSIYY715 pKa = 8.71 EE716 pKa = 4.1 VPSGSFIKK724 pKa = 10.55 VSYY727 pKa = 8.03 FTSPLKK733 pKa = 11.03 NPTVDD738 pKa = 3.4 AYY740 pKa = 11.05 NAEE743 pKa = 4.07 ISRR746 pKa = 11.84 NSAYY750 pKa = 10.6 LIKK753 pKa = 10.85 ANASLDD759 pKa = 3.63 SVAAMLSNISNRR771 pKa = 11.84 IDD773 pKa = 3.08 AMEE776 pKa = 4.43 RR777 pKa = 11.84 LMEE780 pKa = 4.0 PTRR783 pKa = 11.84 AHH785 pKa = 7.2 RR786 pKa = 11.84 IAGVVSSIGGVISLGMPLLGAIVVTIGSIISIADD820 pKa = 3.54 PDD822 pKa = 3.85 KK823 pKa = 11.39 QGIDD827 pKa = 3.48 YY828 pKa = 10.63 HH829 pKa = 7.88 SVANAFMSWCQYY841 pKa = 11.16 AAVCRR846 pKa = 11.84 YY847 pKa = 9.64 EE848 pKa = 4.02 YY849 pKa = 11.43 GLLKK853 pKa = 10.76 RR854 pKa = 11.84 GDD856 pKa = 3.77 EE857 pKa = 4.2 KK858 pKa = 11.55 LDD860 pKa = 3.4 VLSFMPKK867 pKa = 9.56 RR868 pKa = 11.84 VVSDD872 pKa = 3.97 FKK874 pKa = 11.27 NKK876 pKa = 9.57 PDD878 pKa = 3.98 VISLPEE884 pKa = 4.37 LGEE887 pKa = 4.2 SVLRR891 pKa = 11.84 GSSTDD896 pKa = 3.6 YY897 pKa = 11.38 LDD899 pKa = 3.42 TEE901 pKa = 4.34 INIIYY906 pKa = 10.48 NDD908 pKa = 3.89 MQLLGQGKK916 pKa = 10.1 LSDD919 pKa = 3.84 WLNKK923 pKa = 8.73 TVSKK927 pKa = 11.09 VEE929 pKa = 3.79 NNAANFFEE937 pKa = 4.65 RR938 pKa = 11.84 NLVKK942 pKa = 10.66 SLANKK947 pKa = 9.36 EE948 pKa = 4.18 VLPVHH953 pKa = 6.2 ARR955 pKa = 11.84 VEE957 pKa = 4.03 ITQTEE962 pKa = 4.54 KK963 pKa = 10.62 IGDD966 pKa = 3.84 VYY968 pKa = 10.26 RR969 pKa = 11.84 TTILYY974 pKa = 9.13 TGINEE979 pKa = 4.1 GSYY982 pKa = 10.76 LGGDD986 pKa = 3.69 VFASRR991 pKa = 11.84 LGDD994 pKa = 3.7 KK995 pKa = 10.79 NILRR999 pKa = 11.84 MNGFEE1004 pKa = 4.22 SGPGRR1009 pKa = 11.84 FKK1011 pKa = 11.1 AIVEE1015 pKa = 4.43 STTEE1019 pKa = 3.66 VGNFRR1024 pKa = 11.84 VVDD1027 pKa = 3.54 WTVSGMSRR1035 pKa = 11.84 YY1036 pKa = 9.32 EE1037 pKa = 3.54 IYY1039 pKa = 10.65 AAAGEE1044 pKa = 4.64 VYY1046 pKa = 9.95 PSKK1049 pKa = 10.86 DD1050 pKa = 3.21 PSHH1053 pKa = 7.29 ADD1055 pKa = 3.16 VQLLYY1060 pKa = 10.83 EE1061 pKa = 4.53 SIVRR1065 pKa = 11.84 DD1066 pKa = 3.39 LTTRR1070 pKa = 11.84 DD1071 pKa = 2.98 GSFVLKK1077 pKa = 10.58 HH1078 pKa = 6.41 HH1079 pKa = 7.34 DD1080 pKa = 3.65 VLLLPGQLDD1089 pKa = 3.5 AFEE1092 pKa = 4.37 EE1093 pKa = 4.73 LIIKK1097 pKa = 9.07 NASNYY1102 pKa = 9.08 QYY1104 pKa = 11.81 AFIGSNCSKK1113 pKa = 10.84 LCAA1116 pKa = 4.26
Molecular weight: 123.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.629
IPC2_protein 4.724
IPC_protein 4.724
Toseland 4.571
ProMoST 4.863
Dawson 4.711
Bjellqvist 4.851
Wikipedia 4.609
Rodwell 4.584
Grimsley 4.482
Solomon 4.711
Lehninger 4.66
Nozaki 4.813
DTASelect 5.054
Thurlkill 4.584
EMBOSS 4.635
Sillero 4.863
Patrickios 3.427
IPC_peptide 4.711
IPC2_peptide 4.851
IPC2.peptide.svr19 4.824
Protein with the highest isoelectric point:
>sp|Q85443|NSP12_RDVF Non-structural protein 12A OS=Rice dwarf virus (isolate Fujian) OX=142804 PE=2 SV=1
MM1 pKa = 7.79 SGTLPLAMTASEE13 pKa = 4.89 SFVGMQVLAQDD24 pKa = 4.45 KK25 pKa = 9.24 EE26 pKa = 4.54 VKK28 pKa = 9.37 ATFIALDD35 pKa = 3.48 RR36 pKa = 11.84 KK37 pKa = 10.48 LPANLKK43 pKa = 9.22 VPYY46 pKa = 8.92 MKK48 pKa = 10.12 NAKK51 pKa = 9.48 YY52 pKa = 8.07 RR53 pKa = 11.84 TCICPSSNHH62 pKa = 6.32 LVDD65 pKa = 5.24 DD66 pKa = 4.75 CVCEE70 pKa = 4.06 DD71 pKa = 4.05 VIIAYY76 pKa = 7.41 TAHH79 pKa = 7.1 RR80 pKa = 11.84 NNAVAALLYY89 pKa = 10.55 SDD91 pKa = 4.73 GNVIHH96 pKa = 7.52 RR97 pKa = 11.84 SGTLKK102 pKa = 10.56 PKK104 pKa = 9.33 SQNRR108 pKa = 11.84 FDD110 pKa = 4.07 LRR112 pKa = 11.84 GFLTSVNPGEE122 pKa = 4.24 SSKK125 pKa = 11.68 AEE127 pKa = 4.1 AGTSKK132 pKa = 9.31 STQKK136 pKa = 11.05 AYY138 pKa = 10.49 DD139 pKa = 3.85 RR140 pKa = 11.84 KK141 pKa = 10.7 DD142 pKa = 3.14 KK143 pKa = 11.35 SPSKK147 pKa = 10.79 GKK149 pKa = 10.02 NSKK152 pKa = 10.37 KK153 pKa = 10.24 GGKK156 pKa = 9.3 KK157 pKa = 9.86 SSSEE161 pKa = 4.1 RR162 pKa = 11.84 KK163 pKa = 8.84 RR164 pKa = 11.84 KK165 pKa = 9.4 EE166 pKa = 3.57 YY167 pKa = 10.81 SSNSEE172 pKa = 3.84 TDD174 pKa = 3.3 LSSDD178 pKa = 2.88 SDD180 pKa = 3.99 ANTRR184 pKa = 11.84 KK185 pKa = 9.88 SKK187 pKa = 10.83 RR188 pKa = 11.84 KK189 pKa = 9.37
Molecular weight: 20.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.98
IPC2_protein 9.063
IPC_protein 8.96
Toseland 9.955
ProMoST 9.56
Dawson 10.101
Bjellqvist 9.736
Wikipedia 10.218
Rodwell 10.73
Grimsley 10.145
Solomon 10.131
Lehninger 10.101
Nozaki 9.984
DTASelect 9.706
Thurlkill 9.984
EMBOSS 10.335
Sillero 10.028
Patrickios 10.409
IPC_peptide 10.131
IPC2_peptide 8.273
IPC2.peptide.svr19 7.985
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
7840
92
1444
603.1
67.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.722 ± 0.312
1.161 ± 0.117
6.314 ± 0.314
4.579 ± 0.286
4.26 ± 0.243
5.37 ± 0.261
2.092 ± 0.164
6.237 ± 0.218
5.255 ± 0.474
9.592 ± 0.236
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.908 ± 0.112
5.357 ± 0.179
4.26 ± 0.235
3.048 ± 0.181
4.898 ± 0.257
9.63 ± 0.388
6.173 ± 0.301
7.258 ± 0.459
0.842 ± 0.138
4.043 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here