Anguillid herpesvirus 1 (isolate Anguilla anguilla/Netherlands/500138/1998) (AngHV-1) (European eel herpesvirus)
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D2E8A8|D2E8A8_ANHV1 Capsid maturation protease OS=Anguillid herpesvirus 1 (isolate Anguilla anguilla/Netherlands/500138/1998) OX=1283343 GN=AngHV1_ORF57 PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 5.56 AFVQATFEE10 pKa = 4.21 EE11 pKa = 5.15 LPQLVDD17 pKa = 3.42 DD18 pKa = 4.49 RR19 pKa = 11.84 RR20 pKa = 11.84 GDD22 pKa = 3.78 RR23 pKa = 11.84 ILLKK27 pKa = 10.49 RR28 pKa = 11.84 GIVLRR33 pKa = 11.84 IAPLDD38 pKa = 3.9 GTIHH42 pKa = 6.63 WGDD45 pKa = 4.05 DD46 pKa = 4.13 DD47 pKa = 5.62 IVSAWEE53 pKa = 3.96 TLFLPTPEE61 pKa = 4.04 KK62 pKa = 10.34 MVVLGAIDD70 pKa = 4.98 HH71 pKa = 7.3 IDD73 pKa = 3.98 GEE75 pKa = 4.87 WICQVIVLVGEE86 pKa = 4.57 GGCVYY91 pKa = 10.48 FVDD94 pKa = 4.84 ADD96 pKa = 3.95 EE97 pKa = 4.43 LHH99 pKa = 6.53 YY100 pKa = 8.9 MAPSIAEE107 pKa = 4.15 LDD109 pKa = 4.14 TNVSPTTPPIASYY122 pKa = 9.72 TYY124 pKa = 10.79 GQFCEE129 pKa = 4.5 ASAEE133 pKa = 4.07 EE134 pKa = 4.32 RR135 pKa = 11.84 DD136 pKa = 3.69 QHH138 pKa = 6.9 VLIPQATGDD147 pKa = 3.8 FVEE150 pKa = 4.5 SHH152 pKa = 5.89 TEE154 pKa = 3.81 GMLADD159 pKa = 4.4 LEE161 pKa = 4.45 RR162 pKa = 11.84 LNSVV166 pKa = 3.21
Molecular weight: 18.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.126
IPC2_protein 4.088
IPC_protein 4.05
Toseland 3.859
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.935
Rodwell 3.884
Grimsley 3.77
Solomon 4.024
Lehninger 3.973
Nozaki 4.139
DTASelect 4.342
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.177
Patrickios 3.236
IPC_peptide 4.024
IPC2_peptide 4.151
IPC2.peptide.svr19 4.075
Protein with the highest isoelectric point:
>tr|D2E8B5|D2E8B5_ANHV1 Membrane protein ORF64 OS=Anguillid herpesvirus 1 (isolate Anguilla anguilla/Netherlands/500138/1998) OX=1283343 GN=AngHV1_ORF64 PE=4 SV=1
MM1 pKa = 7.0 STIAMLRR8 pKa = 11.84 SVARR12 pKa = 11.84 IAEE15 pKa = 4.35 LTQGSDD21 pKa = 3.69 PNQSTNNNYY30 pKa = 10.01 RR31 pKa = 11.84 PDD33 pKa = 3.46 LSEE36 pKa = 4.26 EE37 pKa = 4.17 EE38 pKa = 4.32 KK39 pKa = 10.86 KK40 pKa = 10.26 DD41 pKa = 4.07 TIRR44 pKa = 11.84 DD45 pKa = 3.73 LQNEE49 pKa = 4.27 MLRR52 pKa = 11.84 ARR54 pKa = 11.84 IEE56 pKa = 3.96 VLARR60 pKa = 11.84 ARR62 pKa = 11.84 DD63 pKa = 3.65 KK64 pKa = 10.68 TAAQALDD71 pKa = 3.53 VEE73 pKa = 4.96 AGRR76 pKa = 11.84 KK77 pKa = 6.27 RR78 pKa = 11.84 TKK80 pKa = 8.69 KK81 pKa = 8.64 TKK83 pKa = 8.95 PARR86 pKa = 11.84 KK87 pKa = 8.64 IAATMTINDD96 pKa = 4.99 LPPIDD101 pKa = 4.8 KK102 pKa = 10.63 AKK104 pKa = 10.66 EE105 pKa = 3.89 MQKK108 pKa = 10.59 RR109 pKa = 11.84 LATKK113 pKa = 9.6 PVEE116 pKa = 4.14 PFSGKK121 pKa = 8.6 GHH123 pKa = 6.53 ILKK126 pKa = 9.8 EE127 pKa = 4.13 VPKK130 pKa = 10.37 RR131 pKa = 11.84 RR132 pKa = 11.84 LEE134 pKa = 4.18 HH135 pKa = 5.39 EE136 pKa = 3.5 HH137 pKa = 7.17 AEE139 pKa = 4.26 RR140 pKa = 11.84 KK141 pKa = 9.55 KK142 pKa = 10.49 LRR144 pKa = 11.84 SYY146 pKa = 11.51 EE147 pKa = 3.97 DD148 pKa = 3.31 LTRR151 pKa = 11.84 SMGMLMLTKK160 pKa = 10.07 PRR162 pKa = 11.84 RR163 pKa = 11.84 SASAWCLVV171 pKa = 3.24
Molecular weight: 19.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.128
IPC2_protein 9.326
IPC_protein 9.385
Toseland 10.511
ProMoST 9.984
Dawson 10.584
Bjellqvist 10.16
Wikipedia 10.687
Rodwell 11.082
Grimsley 10.599
Solomon 10.628
Lehninger 10.613
Nozaki 10.482
DTASelect 10.16
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.496
Patrickios 10.804
IPC_peptide 10.643
IPC2_peptide 8.551
IPC2.peptide.svr19 8.523
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
128
1
129
71176
105
3392
551.8
61.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.087 ± 0.172
1.964 ± 0.114
5.78 ± 0.123
6.159 ± 0.223
3.675 ± 0.093
5.773 ± 0.132
2.317 ± 0.064
4.125 ± 0.122
4.954 ± 0.159
8.858 ± 0.175
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.098
3.799 ± 0.114
6.615 ± 0.208
3.666 ± 0.133
5.812 ± 0.148
6.804 ± 0.195
7.403 ± 0.182
7.212 ± 0.145
1.735 ± 0.084
2.755 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here