Bombyx mori cytoplasmic polyhedrosis virus (BmCPV)
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7EWL8|C7EWL8_CPVBM p101 OS=Bombyx mori cytoplasmic polyhedrosis virus OX=110829 PE=4 SV=1
MM1 pKa = 6.17 TTKK4 pKa = 10.63 LFKK7 pKa = 9.87 QTIIPTNKK15 pKa = 10.21 DD16 pKa = 2.58 VDD18 pKa = 3.49 EE19 pKa = 4.94 KK20 pKa = 11.12 YY21 pKa = 10.44 IYY23 pKa = 10.45 FIEE26 pKa = 4.53 RR27 pKa = 11.84 ADD29 pKa = 3.61 HH30 pKa = 6.96 KK31 pKa = 10.38 IIKK34 pKa = 8.48 PLHH37 pKa = 6.06 PAFEE41 pKa = 4.43 PWQFAGNQHH50 pKa = 5.55 AHH52 pKa = 5.34 QLEE55 pKa = 3.91 QGYY58 pKa = 11.15 ADD60 pKa = 3.18 GHH62 pKa = 5.75 RR63 pKa = 11.84 FITNYY68 pKa = 10.55 NATSRR73 pKa = 11.84 LNSAMTLQIMNKK85 pKa = 9.51 PMITIVKK92 pKa = 9.74 KK93 pKa = 8.79 FTTNTPHH100 pKa = 6.4 QVTTIPEE107 pKa = 4.8 GYY109 pKa = 9.63 SFGVVKK115 pKa = 10.68 EE116 pKa = 4.08 KK117 pKa = 11.13 LCTNEE122 pKa = 3.78 QLLSLLALTAEE133 pKa = 4.29 PVIEE137 pKa = 4.67 NDD139 pKa = 3.93 GEE141 pKa = 4.45 VNDD144 pKa = 5.21 TIQEE148 pKa = 4.13 DD149 pKa = 3.83 EE150 pKa = 4.4 TEE152 pKa = 4.16 KK153 pKa = 11.2 KK154 pKa = 9.42 EE155 pKa = 4.49 VEE157 pKa = 3.95 MRR159 pKa = 11.84 MNWSDD164 pKa = 3.84 EE165 pKa = 4.1 VVEE168 pKa = 4.62 LPKK171 pKa = 10.46 QQEE174 pKa = 4.06 EE175 pKa = 4.62 NVFVLSKK182 pKa = 10.5 PSMISEE188 pKa = 4.29 EE189 pKa = 4.16 EE190 pKa = 4.03 LMPIDD195 pKa = 4.33 MEE197 pKa = 4.49 VMTPKK202 pKa = 10.31 VLEE205 pKa = 4.68 PPTPLPSPIVVAVSSEE221 pKa = 4.13 SPQVKK226 pKa = 9.91 EE227 pKa = 4.49 IEE229 pKa = 4.42 RR230 pKa = 11.84 LPADD234 pKa = 4.26 LPQEE238 pKa = 4.01 QRR240 pKa = 11.84 VSRR243 pKa = 11.84 KK244 pKa = 8.93 PVARR248 pKa = 11.84 TTKK251 pKa = 9.8 PSFSSFSSPDD261 pKa = 2.97 IVSANVQTPVPKK273 pKa = 9.97 VAPRR277 pKa = 11.84 PVEE280 pKa = 3.94 EE281 pKa = 4.35 FLSSVFTSLFEE292 pKa = 3.92 AWFVRR297 pKa = 11.84 VLPLIKK303 pKa = 9.9 QGKK306 pKa = 6.86 PVYY309 pKa = 9.73 MMPGTIKK316 pKa = 10.19 WEE318 pKa = 4.08 RR319 pKa = 11.84 EE320 pKa = 3.79 AQITDD325 pKa = 3.01 TKK327 pKa = 10.21 TRR329 pKa = 11.84 YY330 pKa = 9.34 FNSVSGKK337 pKa = 10.14 LILVEE342 pKa = 4.14 MEE344 pKa = 4.35 SITRR348 pKa = 11.84 ALQPGEE354 pKa = 4.0 LNDD357 pKa = 3.47 RR358 pKa = 11.84 RR359 pKa = 11.84 EE360 pKa = 4.77 LIRR363 pKa = 11.84 QTLEE367 pKa = 4.13 TVCAGHH373 pKa = 6.79 NIHH376 pKa = 6.6 VLTGKK381 pKa = 8.76 DD382 pKa = 3.05 TFGVLEE388 pKa = 4.36 VEE390 pKa = 4.51
Molecular weight: 44.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.255
IPC2_protein 5.258
IPC_protein 5.207
Toseland 5.207
ProMoST 5.283
Dawson 5.207
Bjellqvist 5.334
Wikipedia 5.054
Rodwell 5.143
Grimsley 5.156
Solomon 5.207
Lehninger 5.169
Nozaki 5.334
DTASelect 5.423
Thurlkill 5.207
EMBOSS 5.118
Sillero 5.423
Patrickios 4.406
IPC_peptide 5.219
IPC2_peptide 5.423
IPC2.peptide.svr19 5.369
Protein with the highest isoelectric point:
>tr|D0EZK6|D0EZK6_CPVBM RNA-dependent RNA polymerase OS=Bombyx mori cytoplasmic polyhedrosis virus OX=110829 PE=1 SV=1
MM1 pKa = 7.39 FAIDD5 pKa = 4.05 PLKK8 pKa = 10.53 HH9 pKa = 5.65 SKK11 pKa = 10.57 LYY13 pKa = 10.59 EE14 pKa = 4.03 EE15 pKa = 4.63 YY16 pKa = 10.94 GLYY19 pKa = 10.45 LRR21 pKa = 11.84 PHH23 pKa = 6.9 QINQEE28 pKa = 3.99 IKK30 pKa = 8.54 PTTIKK35 pKa = 10.54 KK36 pKa = 10.23 KK37 pKa = 10.17 EE38 pKa = 3.85 LAPTIRR44 pKa = 11.84 SIKK47 pKa = 8.95 YY48 pKa = 9.15 ASLIHH53 pKa = 7.22 SMLAEE58 pKa = 3.8 HH59 pKa = 7.52 AARR62 pKa = 11.84 HH63 pKa = 5.84 NGTLINPRR71 pKa = 11.84 MYY73 pKa = 11.37 ADD75 pKa = 5.11 MITLGNTKK83 pKa = 8.74 VTVTKK88 pKa = 8.63 GTPKK92 pKa = 10.7 AQIDD96 pKa = 3.92 TLKK99 pKa = 10.59 MNGLTVVSKK108 pKa = 10.56 SRR110 pKa = 11.84 RR111 pKa = 11.84 NNKK114 pKa = 9.1 KK115 pKa = 10.34 KK116 pKa = 10.17 PVSDD120 pKa = 3.47 TTATIDD126 pKa = 3.48 EE127 pKa = 4.35 NTNAIVTYY135 pKa = 10.29 KK136 pKa = 10.83 ALTEE140 pKa = 3.92 MSTLIEE146 pKa = 4.27 SFRR149 pKa = 11.84 LPSGLTLIIFDD160 pKa = 3.97 DD161 pKa = 4.06 EE162 pKa = 5.1 KK163 pKa = 10.96 YY164 pKa = 10.59 QSLIPNYY171 pKa = 8.93 INQLIAYY178 pKa = 5.86 TQPHH182 pKa = 7.71 IIPTWQGIADD192 pKa = 4.32 FSDD195 pKa = 3.56 TYY197 pKa = 10.93 LRR199 pKa = 11.84 SYY201 pKa = 10.09 FKK203 pKa = 10.89 RR204 pKa = 11.84 PFEE207 pKa = 4.06 LTASNLAAPQKK218 pKa = 10.75 HH219 pKa = 5.88 NLSPMTRR226 pKa = 11.84 SIFNNTGRR234 pKa = 11.84 EE235 pKa = 3.82 DD236 pKa = 3.4 AVIRR240 pKa = 11.84 KK241 pKa = 9.45 LYY243 pKa = 10.72 GYY245 pKa = 10.27 GEE247 pKa = 4.44 YY248 pKa = 11.12 VFIKK252 pKa = 11.0 YY253 pKa = 8.71 EE254 pKa = 4.09 GCLITWTGIYY264 pKa = 10.78 GEE266 pKa = 4.2 VTMMVNLSKK275 pKa = 10.66 RR276 pKa = 11.84 DD277 pKa = 3.55 LGLDD281 pKa = 3.25 VGDD284 pKa = 5.72 DD285 pKa = 3.58 YY286 pKa = 11.97 LKK288 pKa = 10.45 EE289 pKa = 4.1 YY290 pKa = 10.77 KK291 pKa = 10.13 KK292 pKa = 10.91 LLFYY296 pKa = 10.92 GVITDD301 pKa = 5.76 AIPSGISTRR310 pKa = 11.84 STIMKK315 pKa = 9.91 ISPHH319 pKa = 7.59 KK320 pKa = 10.14 MMNPSGGALAVLSKK334 pKa = 10.58 FLEE337 pKa = 4.48 AVVSTNVINATLVVYY352 pKa = 10.51 AEE354 pKa = 4.34 KK355 pKa = 10.85 GAGKK359 pKa = 8.68 TSFLSTYY366 pKa = 10.93 AEE368 pKa = 4.08 QLSLASGQVVGHH380 pKa = 6.99 LSSDD384 pKa = 3.14 AYY386 pKa = 10.61 GRR388 pKa = 11.84 WLAKK392 pKa = 9.89 TKK394 pKa = 10.46 DD395 pKa = 3.21 IEE397 pKa = 4.52 EE398 pKa = 4.17 PSFAYY403 pKa = 10.15 DD404 pKa = 3.46 YY405 pKa = 11.4 VLSLDD410 pKa = 3.57 TDD412 pKa = 4.2 DD413 pKa = 4.63 NEE415 pKa = 4.92 SYY417 pKa = 10.91 YY418 pKa = 9.29 EE419 pKa = 4.04 QKK421 pKa = 10.93 ASEE424 pKa = 4.65 LLMSHH429 pKa = 7.16 GISEE433 pKa = 4.36 VAQYY437 pKa = 11.15 EE438 pKa = 4.28 LLSVRR443 pKa = 11.84 KK444 pKa = 8.85 KK445 pKa = 10.38 IKK447 pKa = 10.4 MMDD450 pKa = 3.36 EE451 pKa = 4.0 MNEE454 pKa = 3.76 VLIAQLEE461 pKa = 4.3 NADD464 pKa = 3.67 THH466 pKa = 6.13 SEE468 pKa = 4.04 RR469 pKa = 11.84 NFYY472 pKa = 11.58 YY473 pKa = 9.9 MVSTGKK479 pKa = 6.19 TTPRR483 pKa = 11.84 ILIVEE488 pKa = 4.06 GHH490 pKa = 6.41 FNAQDD495 pKa = 3.26 ATIARR500 pKa = 11.84 TDD502 pKa = 3.41 TTVLLRR508 pKa = 11.84 TINDD512 pKa = 3.35 TTQAMRR518 pKa = 11.84 DD519 pKa = 3.56 RR520 pKa = 11.84 QRR522 pKa = 11.84 GGVVQLFLRR531 pKa = 11.84 DD532 pKa = 3.14 TYY534 pKa = 11.61 YY535 pKa = 11.31 RR536 pKa = 11.84 LLPALHH542 pKa = 5.25 TTVYY546 pKa = 8.96 PFEE549 pKa = 4.09 MLEE552 pKa = 4.54 SIKK555 pKa = 10.15 RR556 pKa = 11.84 WKK558 pKa = 8.44 WVHH561 pKa = 5.43
Molecular weight: 63.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.04
IPC2_protein 8.185
IPC_protein 8.126
Toseland 8.624
ProMoST 8.521
Dawson 8.975
Bjellqvist 8.741
Wikipedia 9.18
Rodwell 9.077
Grimsley 9.048
Solomon 9.048
Lehninger 8.99
Nozaki 8.653
DTASelect 8.726
Thurlkill 8.785
EMBOSS 9.077
Sillero 8.916
Patrickios 4.266
IPC_peptide 9.033
IPC2_peptide 7.395
IPC2.peptide.svr19 7.563
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
8294
248
1333
754.0
85.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.86 ± 0.476
0.699 ± 0.177
5.836 ± 0.22
5.45 ± 0.318
3.786 ± 0.208
4.702 ± 0.253
2.64 ± 0.119
6.788 ± 0.264
4.292 ± 0.404
8.934 ± 0.387
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.894 ± 0.19
5.619 ± 0.242
4.365 ± 0.364
3.81 ± 0.2
6.004 ± 0.273
7.801 ± 0.322
7.403 ± 0.395
6.933 ± 0.353
0.796 ± 0.088
4.389 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here