Sulfitobacter marinus
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3434 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6QA08|A0A1I6QA08_9RHOB Iron-sulfur cluster carrier protein OS=Sulfitobacter marinus OX=394264 GN=SAMN04488040_0600 PE=3 SV=1
MM1 pKa = 7.58 NDD3 pKa = 2.84 STQPLEE9 pKa = 4.24 GTPLIAPSTTDD20 pKa = 2.86 HH21 pKa = 7.13 PLYY24 pKa = 9.83 EE25 pKa = 4.51 QIVEE29 pKa = 4.24 ACRR32 pKa = 11.84 TVYY35 pKa = 10.74 DD36 pKa = 3.74 PEE38 pKa = 5.09 IPVNIYY44 pKa = 10.39 EE45 pKa = 4.82 LGLIYY50 pKa = 10.32 TIDD53 pKa = 3.6 INPEE57 pKa = 3.71 SEE59 pKa = 4.56 VNIKK63 pKa = 9.85 MSLTAPGCPVAGEE76 pKa = 4.15 MPGWVADD83 pKa = 3.91 AVEE86 pKa = 4.55 PLPGVKK92 pKa = 9.08 TVDD95 pKa = 3.42 VEE97 pKa = 4.99 LVWEE101 pKa = 4.91 PPWGMDD107 pKa = 3.35 MMSDD111 pKa = 3.68 EE112 pKa = 4.89 ARR114 pKa = 11.84 LEE116 pKa = 4.14 LGFMM120 pKa = 4.54
Molecular weight: 13.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.757
IPC_protein 3.681
Toseland 3.503
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.554
Rodwell 3.516
Grimsley 3.414
Solomon 3.63
Lehninger 3.579
Nozaki 3.783
DTASelect 3.91
Thurlkill 3.541
EMBOSS 3.567
Sillero 3.795
Patrickios 0.769
IPC_peptide 3.63
IPC2_peptide 3.77
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A1I6VQ68|A0A1I6VQ68_9RHOB TVP38/TMEM64 family membrane protein OS=Sulfitobacter marinus OX=394264 GN=SAMN04488040_3501 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 8.52 EE41 pKa = 3.76 LSAA44 pKa = 4.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3434
0
3434
1079271
30
1510
314.3
34.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.78 ± 0.054
0.892 ± 0.013
6.261 ± 0.034
5.429 ± 0.037
3.808 ± 0.026
8.487 ± 0.042
2.056 ± 0.021
5.636 ± 0.031
3.653 ± 0.034
9.809 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.02 ± 0.024
2.929 ± 0.021
4.877 ± 0.026
3.465 ± 0.023
5.941 ± 0.033
5.375 ± 0.031
5.7 ± 0.025
7.245 ± 0.03
1.348 ± 0.018
2.288 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here