Wenzhou tapeworm virus 1
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KN32|A0A1L3KN32_9MONO Putative glycoprotein OS=Wenzhou tapeworm virus 1 OX=1923661 PE=4 SV=1
MM1 pKa = 6.93 STITRR6 pKa = 11.84 SSKK9 pKa = 10.38 RR10 pKa = 11.84 SGSEE14 pKa = 3.51 LSGSARR20 pKa = 11.84 KK21 pKa = 7.91 YY22 pKa = 9.35 QRR24 pKa = 11.84 YY25 pKa = 8.89 NPEE28 pKa = 4.33 DD29 pKa = 3.69 LQEE32 pKa = 4.57 GADD35 pKa = 3.83 LDD37 pKa = 3.97 YY38 pKa = 11.53 QEE40 pKa = 4.65 WQFGSHH46 pKa = 6.14 GSVPTTVEE54 pKa = 3.57 EE55 pKa = 4.79 KK56 pKa = 10.63 YY57 pKa = 10.77 AALALQDD64 pKa = 3.86 QEE66 pKa = 4.59 STSAQGPQDD75 pKa = 3.75 PATVSTTDD83 pKa = 3.52 PAVQILDD90 pKa = 3.66 QDD92 pKa = 4.11 SSNQTPHH99 pKa = 6.78 PALVVMLGRR108 pKa = 11.84 IVANQEE114 pKa = 3.99 RR115 pKa = 11.84 IIEE118 pKa = 3.97 LLQRR122 pKa = 11.84 IEE124 pKa = 3.7 AQQRR128 pKa = 11.84 HH129 pKa = 5.21 FADD132 pKa = 4.15 SEE134 pKa = 4.5 KK135 pKa = 11.02 AWTEE139 pKa = 3.56 ALNATKK145 pKa = 10.21 TSLIEE150 pKa = 4.15 LYY152 pKa = 9.75 RR153 pKa = 11.84 QSSVAIVRR161 pKa = 11.84 PSQTSKK167 pKa = 10.43 PAPLLQAPTATFLSNPSVADD187 pKa = 3.88 ANASAFNKK195 pKa = 9.49 FFF197 pKa = 4.5
Molecular weight: 21.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.06
IPC2_protein 5.092
IPC_protein 4.978
Toseland 4.889
ProMoST 5.092
Dawson 4.94
Bjellqvist 5.08
Wikipedia 4.813
Rodwell 4.863
Grimsley 4.813
Solomon 4.94
Lehninger 4.902
Nozaki 5.067
DTASelect 5.207
Thurlkill 4.889
EMBOSS 4.838
Sillero 5.13
Patrickios 4.279
IPC_peptide 4.952
IPC2_peptide 5.13
IPC2.peptide.svr19 5.142
Protein with the highest isoelectric point:
>tr|A0A1L3KN08|A0A1L3KN08_9MONO GDP polyribonucleotidyltransferase OS=Wenzhou tapeworm virus 1 OX=1923661 PE=4 SV=1
MM1 pKa = 7.65 IKK3 pKa = 10.57 NILRR7 pKa = 11.84 DD8 pKa = 3.52 SGTSILSGSVVHH20 pKa = 6.47 SLGGLVSTVTGGLGVTAIYY39 pKa = 10.5 KK40 pKa = 9.61 KK41 pKa = 11.13 VKK43 pKa = 10.33 DD44 pKa = 3.44 RR45 pKa = 11.84 WAKK48 pKa = 9.68 KK49 pKa = 10.02 QEE51 pKa = 4.35 AGCTTIKK58 pKa = 10.36 FKK60 pKa = 11.08 HH61 pKa = 5.69 FTTGPDD67 pKa = 3.29 GTFCICGDD75 pKa = 3.15 KK76 pKa = 11.0 HH77 pKa = 6.99 KK78 pKa = 10.16 FTGHH82 pKa = 6.65 LVLLTTPGDD91 pKa = 4.02 EE92 pKa = 5.01 LGHH95 pKa = 6.81 LPTGHH100 pKa = 5.38 YY101 pKa = 9.45 HH102 pKa = 5.3 KK103 pKa = 10.58 QAMVVCLAPQCMDD116 pKa = 3.6 NNVQNPEE123 pKa = 4.19 EE124 pKa = 4.4 LCLLPAHH131 pKa = 7.03 ASSII135 pKa = 3.95
Molecular weight: 14.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.353
IPC2_protein 7.556
IPC_protein 7.497
Toseland 7.161
ProMoST 8.009
Dawson 8.156
Bjellqvist 8.565
Wikipedia 8.068
Rodwell 8.17
Grimsley 7.205
Solomon 8.273
Lehninger 8.302
Nozaki 8.946
DTASelect 8.273
Thurlkill 8.331
EMBOSS 8.39
Sillero 8.697
Patrickios 4.279
IPC_peptide 8.273
IPC2_peptide 7.717
IPC2.peptide.svr19 7.606
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3448
135
1961
689.6
77.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.874 ± 0.671
2.001 ± 0.305
5.191 ± 0.294
5.626 ± 0.231
3.77 ± 0.323
5.307 ± 0.469
2.958 ± 0.285
5.916 ± 0.394
3.944 ± 0.457
10.267 ± 0.898
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.349
3.248 ± 0.174
5.8 ± 0.461
3.828 ± 0.607
6.497 ± 0.572
7.831 ± 0.591
6.061 ± 0.607
6.932 ± 0.941
1.508 ± 0.397
3.886 ± 0.489
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here