Hibiscus latent Singapore virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8BE68|Q8BE68_9VIRU Capsid protein OS=Hibiscus latent Singapore virus OX=185955 PE=3 SV=2
MM1 pKa = 8.1PYY3 pKa = 11.15LNLTPLNLIYY13 pKa = 10.52TSGTFAPYY21 pKa = 10.67DD22 pKa = 3.55VFLEE26 pKa = 3.96ILVKK30 pKa = 10.62SRR32 pKa = 11.84SNSFQTQAGRR42 pKa = 11.84DD43 pKa = 3.67TLRR46 pKa = 11.84EE47 pKa = 3.75QLINSLQIVANLNTRR62 pKa = 11.84YY63 pKa = 9.19PLLGFYY69 pKa = 10.36VWVRR73 pKa = 11.84NPTLAPVFEE82 pKa = 4.7ALLRR86 pKa = 11.84ATDD89 pKa = 3.49TKK91 pKa = 11.27NRR93 pKa = 11.84IIEE96 pKa = 4.15VEE98 pKa = 4.16EE99 pKa = 3.96EE100 pKa = 4.24SRR102 pKa = 11.84PTTAEE107 pKa = 3.91TLNATQRR114 pKa = 11.84VDD116 pKa = 3.35DD117 pKa = 4.06ATVAIHH123 pKa = 7.12KK124 pKa = 9.93EE125 pKa = 3.34IDD127 pKa = 3.72NILLLLQGGTAVYY140 pKa = 10.62DD141 pKa = 3.52RR142 pKa = 11.84TAFEE146 pKa = 4.53VVSGLSWADD155 pKa = 3.24PTTTSTTTT163 pKa = 3.0

Molecular weight: 18.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5K6D3|Q5K6D3_9VIRU Methyltransferase/RNA helicase OS=Hibiscus latent Singapore virus OX=185955 PE=4 SV=2
MM1 pKa = 6.69TTDD4 pKa = 3.39IKK6 pKa = 10.91QQITNVRR13 pKa = 11.84DD14 pKa = 3.8DD15 pKa = 3.77ACQGASSLIKK25 pKa = 10.39GLATRR30 pKa = 11.84RR31 pKa = 11.84VYY33 pKa = 11.07DD34 pKa = 3.66EE35 pKa = 4.87AVQTLTVLDD44 pKa = 4.24KK45 pKa = 10.89RR46 pKa = 11.84PKK48 pKa = 8.45FTFSNMVTADD58 pKa = 3.29QAKK61 pKa = 9.53LVTEE65 pKa = 5.43AYY67 pKa = 9.62PEE69 pKa = 4.1FNISYY74 pKa = 9.43VGNRR78 pKa = 11.84NAVHH82 pKa = 6.3SLAGGLRR89 pKa = 11.84KK90 pKa = 9.93LEE92 pKa = 4.02MEE94 pKa = 4.48YY95 pKa = 11.29LMTLVPYY102 pKa = 10.13GSPTYY107 pKa = 10.47DD108 pKa = 2.35IGGNYY113 pKa = 8.97AQHH116 pKa = 6.47LLKK119 pKa = 10.62GRR121 pKa = 11.84SYY123 pKa = 9.37VHH125 pKa = 6.8CCNPCLDD132 pKa = 3.98FRR134 pKa = 11.84DD135 pKa = 3.48IARR138 pKa = 11.84NEE140 pKa = 4.08NYY142 pKa = 10.12KK143 pKa = 9.05EE144 pKa = 4.19TLDD147 pKa = 3.99SYY149 pKa = 10.89LKK151 pKa = 10.75RR152 pKa = 11.84FTTPDD157 pKa = 3.76CGWFSAACRR166 pKa = 11.84KK167 pKa = 7.71VTARR171 pKa = 11.84PLPYY175 pKa = 9.71FQQDD179 pKa = 2.41AFEE182 pKa = 5.22RR183 pKa = 11.84YY184 pKa = 8.52RR185 pKa = 11.84QNPKK189 pKa = 10.62DD190 pKa = 3.94VTCSEE195 pKa = 4.47PFQDD199 pKa = 5.33CCMQPPSNQDD209 pKa = 2.85CFAISVHH216 pKa = 5.68SLYY219 pKa = 10.65DD220 pKa = 3.34IPVDD224 pKa = 3.68EE225 pKa = 5.63LGAALLRR232 pKa = 11.84KK233 pKa = 9.05NVKK236 pKa = 8.05TLYY239 pKa = 10.39AALHH243 pKa = 5.72FSEE246 pKa = 4.79EE247 pKa = 4.27LLMGATEE254 pKa = 4.24ARR256 pKa = 11.84LGTIGAKK263 pKa = 9.98FFRR266 pKa = 11.84EE267 pKa = 3.83GDD269 pKa = 3.51EE270 pKa = 4.13VTFGFDD276 pKa = 3.67GEE278 pKa = 4.6STLLYY283 pKa = 9.15THH285 pKa = 6.41SFKK288 pKa = 11.17NICAYY293 pKa = 8.33ITRR296 pKa = 11.84SFFYY300 pKa = 10.31AGARR304 pKa = 11.84FAYY307 pKa = 9.16MKK309 pKa = 9.85EE310 pKa = 3.79FLVRR314 pKa = 11.84RR315 pKa = 11.84VDD317 pKa = 3.4TVYY320 pKa = 11.16CKK322 pKa = 10.55FIKK325 pKa = 9.94IDD327 pKa = 3.62TQCLYY332 pKa = 11.07KK333 pKa = 10.63SVFHH337 pKa = 6.82TDD339 pKa = 2.56IDD341 pKa = 3.93NEE343 pKa = 4.07NMIKK347 pKa = 10.66GMDD350 pKa = 3.54EE351 pKa = 3.76AFMYY355 pKa = 10.28KK356 pKa = 9.69RR357 pKa = 11.84EE358 pKa = 4.02AALLNAARR366 pKa = 11.84PIFKK370 pKa = 10.28DD371 pKa = 3.13KK372 pKa = 11.07AAFRR376 pKa = 11.84VWFPNSVGKK385 pKa = 10.46VIIPFFKK392 pKa = 10.72GSSCTSAKK400 pKa = 10.55VKK402 pKa = 10.0VKK404 pKa = 10.42KK405 pKa = 10.49RR406 pKa = 11.84LVDD409 pKa = 3.37EE410 pKa = 5.35DD411 pKa = 4.56FVFTVLNHH419 pKa = 5.27IRR421 pKa = 11.84TYY423 pKa = 10.63SGKK426 pKa = 8.98QLSYY430 pKa = 11.05EE431 pKa = 4.06NVLSFVEE438 pKa = 4.76SIRR441 pKa = 11.84SRR443 pKa = 11.84VVANGANVRR452 pKa = 11.84SEE454 pKa = 3.23WDD456 pKa = 3.2IPKK459 pKa = 10.2SEE461 pKa = 4.09IQDD464 pKa = 3.03IAMSLFLITRR474 pKa = 11.84LRR476 pKa = 11.84TLQDD480 pKa = 3.56SNVLQQFSVKK490 pKa = 10.12GKK492 pKa = 10.46SLTDD496 pKa = 3.6LVKK499 pKa = 10.33EE500 pKa = 4.02ALGGLVGSMFEE511 pKa = 4.96PLTQVCIDD519 pKa = 3.79RR520 pKa = 11.84GWFKK524 pKa = 10.75LAEE527 pKa = 4.18HH528 pKa = 5.61TLKK531 pKa = 11.1VEE533 pKa = 4.16VPEE536 pKa = 4.41LSQTFEE542 pKa = 4.84SYY544 pKa = 9.05MQTEE548 pKa = 4.7FSEE551 pKa = 4.66SKK553 pKa = 10.55AIEE556 pKa = 4.24ALDD559 pKa = 3.23VWSYY563 pKa = 11.69LDD565 pKa = 4.65EE566 pKa = 4.88SNQLYY571 pKa = 7.96THH573 pKa = 6.26VSKK576 pKa = 11.02LMEE579 pKa = 5.32KK580 pKa = 9.54YY581 pKa = 9.15TMPDD585 pKa = 2.7FDD587 pKa = 3.76VEE589 pKa = 4.55KK590 pKa = 10.71FRR592 pKa = 11.84EE593 pKa = 4.25LCALLKK599 pKa = 10.6VGPNVISTVIEE610 pKa = 3.97AVLDD614 pKa = 3.62NSLGFTIVGGKK625 pKa = 9.27PEE627 pKa = 4.14HH628 pKa = 6.43QYY630 pKa = 10.96SDD632 pKa = 3.25EE633 pKa = 4.11MKK635 pKa = 10.54AAVAVSSEE643 pKa = 4.02YY644 pKa = 11.26GKK646 pKa = 11.05GKK648 pKa = 10.33EE649 pKa = 4.16YY650 pKa = 11.38GNIAAAASTYY660 pKa = 9.74TPEE663 pKa = 4.93GPLKK667 pKa = 10.78KK668 pKa = 10.4EE669 pKa = 3.95WVKK672 pKa = 11.24KK673 pKa = 10.46NAVQMPLQATTEE685 pKa = 4.15KK686 pKa = 10.46VQRR689 pKa = 11.84FHH691 pKa = 8.46LSEE694 pKa = 4.13EE695 pKa = 4.34SEE697 pKa = 4.49HH698 pKa = 7.74INVEE702 pKa = 3.94NLHH705 pKa = 5.85MVNTSVFTKK714 pKa = 10.79AKK716 pKa = 10.44ACLLYY721 pKa = 10.56TGTLKK726 pKa = 10.22EE727 pKa = 4.08QQMKK731 pKa = 10.85NYY733 pKa = 10.08LDD735 pKa = 3.77YY736 pKa = 11.38LSASICATISNIEE749 pKa = 4.25KK750 pKa = 10.28VLHH753 pKa = 6.92DD754 pKa = 3.94YY755 pKa = 7.71WTTGTQQYY763 pKa = 9.99QSYY766 pKa = 9.85GLWDD770 pKa = 3.56VKK772 pKa = 10.44KK773 pKa = 10.14KK774 pKa = 10.49KK775 pKa = 9.06WVLCPPTAGHH785 pKa = 6.58AWGVAAIKK793 pKa = 10.02GKK795 pKa = 9.2HH796 pKa = 6.32HH797 pKa = 6.33IVMLNFDD804 pKa = 4.86GNDD807 pKa = 3.3QPICDD812 pKa = 4.51KK813 pKa = 10.84EE814 pKa = 3.89WDD816 pKa = 4.19LVCVSNDD823 pKa = 2.95TKK825 pKa = 11.1LFSTMKK831 pKa = 10.24ILEE834 pKa = 4.25NLQGCKK840 pKa = 9.3IQEE843 pKa = 4.18PKK845 pKa = 11.06AKK847 pKa = 8.54ITLVDD852 pKa = 4.09GVPGCGKK859 pKa = 8.26TAEE862 pKa = 4.46ILSKK866 pKa = 11.13ADD868 pKa = 3.89FKK870 pKa = 11.31RR871 pKa = 11.84DD872 pKa = 3.86LIITQGKK879 pKa = 7.69QAAAMIRR886 pKa = 11.84KK887 pKa = 8.82RR888 pKa = 11.84ANANDD893 pKa = 3.59PTRR896 pKa = 11.84PANNDD901 pKa = 2.65NVRR904 pKa = 11.84TVDD907 pKa = 3.64SFLMNPKK914 pKa = 9.09PFEE917 pKa = 4.16YY918 pKa = 10.01KK919 pKa = 10.64VLWIDD924 pKa = 3.46EE925 pKa = 4.22GLMLHH930 pKa = 6.51TGAISFCVALSHH942 pKa = 7.33CEE944 pKa = 3.63QCYY947 pKa = 9.86VFGDD951 pKa = 3.73TQQIPFINRR960 pKa = 11.84VMNFDD965 pKa = 3.77YY966 pKa = 10.5PKK968 pKa = 9.93TLQTLVTDD976 pKa = 3.85SVEE979 pKa = 3.86KK980 pKa = 10.64RR981 pKa = 11.84RR982 pKa = 11.84ITSRR986 pKa = 11.84CPLDD990 pKa = 3.49VTCYY994 pKa = 10.04LAQRR998 pKa = 11.84YY999 pKa = 7.89KK1000 pKa = 11.23GPVVSTNNVEE1010 pKa = 4.04RR1011 pKa = 11.84SLDD1014 pKa = 3.54TKK1016 pKa = 10.86LVAGAARR1023 pKa = 11.84FEE1025 pKa = 4.34PQLTPLPGKK1034 pKa = 10.6VITFTQADD1042 pKa = 4.03KK1043 pKa = 10.1EE1044 pKa = 4.63TLKK1047 pKa = 10.89KK1048 pKa = 10.26RR1049 pKa = 11.84GYY1051 pKa = 9.31QDD1053 pKa = 2.71VHH1055 pKa = 5.87TVHH1058 pKa = 6.77EE1059 pKa = 4.7VQGEE1063 pKa = 4.8TYY1065 pKa = 10.19TEE1067 pKa = 4.07VSLVRR1072 pKa = 11.84LTPTPLSIISPDD1084 pKa = 3.55SPHH1087 pKa = 7.47VLVSLSRR1094 pKa = 11.84HH1095 pKa = 5.22KK1096 pKa = 11.03NRR1098 pKa = 11.84LVYY1101 pKa = 8.57YY1102 pKa = 8.36TVVNDD1107 pKa = 5.0CITYY1111 pKa = 10.59AIGEE1115 pKa = 4.26LAKK1118 pKa = 10.84LNNFMFDD1125 pKa = 2.97MYY1127 pKa = 10.61TVEE1130 pKa = 4.03NQKK1133 pKa = 10.85AA1134 pKa = 3.17

Molecular weight: 128.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3218

163

1639

804.5

90.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.681 ± 0.302

2.3 ± 0.449

5.966 ± 0.247

5.656 ± 0.209

5.034 ± 0.289

5.158 ± 0.289

2.051 ± 0.273

5.19 ± 0.191

7.396 ± 0.757

9.229 ± 0.613

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.247

4.786 ± 0.22

3.884 ± 0.169

3.915 ± 0.082

4.288 ± 0.255

6.588 ± 0.419

7.085 ± 0.881

7.551 ± 0.173

1.025 ± 0.045

4.04 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski