Hubei virga-like virus 2
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJW6|A0A1L3KJW6_9VIRU Capsid protein OS=Hubei virga-like virus 2 OX=1923335 PE=4 SV=1
MM1 pKa = 7.59 FSPNFYY7 pKa = 10.45 RR8 pKa = 11.84 VFEE11 pKa = 4.16 VDD13 pKa = 3.78 LRR15 pKa = 11.84 TLFSPLHH22 pKa = 6.12 PSRR25 pKa = 11.84 SPSLLSLEE33 pKa = 4.66 SDD35 pKa = 3.92 DD36 pKa = 5.55 DD37 pKa = 4.09 VPAAMQYY44 pKa = 10.68 VNWNTCQHH52 pKa = 6.21 RR53 pKa = 11.84 PALTAAARR61 pKa = 11.84 RR62 pKa = 11.84 HH63 pKa = 5.14 KK64 pKa = 9.8 WCSLVDD70 pKa = 3.47 LCNFVEE76 pKa = 4.07 AHH78 pKa = 6.0 KK79 pKa = 10.8 MNGFHH84 pKa = 5.79 THH86 pKa = 5.88 ASRR89 pKa = 11.84 NVVVAAIARR98 pKa = 11.84 LRR100 pKa = 11.84 THH102 pKa = 6.92 APFSRR107 pKa = 11.84 TEE109 pKa = 3.93 RR110 pKa = 11.84 FPEE113 pKa = 4.1 DD114 pKa = 3.49 VEE116 pKa = 5.45 FVDD119 pKa = 5.76 LDD121 pKa = 3.71 ATSVLPYY128 pKa = 9.87 IDD130 pKa = 4.84 QLHH133 pKa = 5.21 QACDD137 pKa = 3.68 LNDD140 pKa = 3.56 RR141 pKa = 11.84 AVNVGNAQPNGSTNDD156 pKa = 3.06 AKK158 pKa = 10.82 RR159 pKa = 11.84 AFDD162 pKa = 3.45 VAFTKK167 pKa = 10.33 IRR169 pKa = 11.84 DD170 pKa = 3.31 FATAVDD176 pKa = 4.36 FPEE179 pKa = 5.39 GSGVWTRR186 pKa = 11.84 EE187 pKa = 3.89 TFEE190 pKa = 4.13 ARR192 pKa = 11.84 YY193 pKa = 9.39 GLEE196 pKa = 3.77 WGNPPAANNN205 pKa = 3.55
Molecular weight: 23.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.686
IPC2_protein 5.575
IPC_protein 5.601
Toseland 6.046
ProMoST 5.957
Dawson 5.868
Bjellqvist 5.881
Wikipedia 5.881
Rodwell 5.855
Grimsley 6.198
Solomon 5.868
Lehninger 5.868
Nozaki 6.135
DTASelect 6.338
Thurlkill 6.338
EMBOSS 6.3
Sillero 6.237
Patrickios 4.202
IPC_peptide 5.881
IPC2_peptide 6.262
IPC2.peptide.svr19 6.173
Protein with the highest isoelectric point:
>tr|A0A1L3KJW6|A0A1L3KJW6_9VIRU Capsid protein OS=Hubei virga-like virus 2 OX=1923335 PE=4 SV=1
MM1 pKa = 7.48 PTFPNQRR8 pKa = 11.84 KK9 pKa = 6.56 TLASAKK15 pKa = 9.78 PSRR18 pKa = 11.84 TPRR21 pKa = 11.84 DD22 pKa = 3.45 GTKK25 pKa = 8.55 VTEE28 pKa = 4.01 KK29 pKa = 10.51 RR30 pKa = 11.84 GYY32 pKa = 10.22 CYY34 pKa = 10.45 LALFEE39 pKa = 5.05 AFNASSEE46 pKa = 4.23 KK47 pKa = 10.57 KK48 pKa = 10.5 LDD50 pKa = 3.43 VASIKK55 pKa = 10.64 ARR57 pKa = 11.84 LGAFPLVRR65 pKa = 11.84 RR66 pKa = 11.84 VVGEE70 pKa = 4.17 LYY72 pKa = 11.05 AHH74 pKa = 5.39 VTFDD78 pKa = 4.38 LFVPCVRR85 pKa = 11.84 RR86 pKa = 11.84 VSNTMFHH93 pKa = 6.24 VDD95 pKa = 2.97 EE96 pKa = 4.96 WRR98 pKa = 11.84 PPMLFSEE105 pKa = 4.75 VLAMTIFSSARR116 pKa = 11.84 IGADD120 pKa = 3.23 DD121 pKa = 4.24 RR122 pKa = 11.84 AHH124 pKa = 6.87 LQQQQLIRR132 pKa = 11.84 VQDD135 pKa = 3.69 LCKK138 pKa = 9.88 TAGLDD143 pKa = 3.66 RR144 pKa = 11.84 NTVLADD150 pKa = 3.5 AAKK153 pKa = 9.93 KK154 pKa = 10.18 CLSRR158 pKa = 11.84 ALAAPDD164 pKa = 3.67 SEE166 pKa = 4.29 MNAIFEE172 pKa = 4.47 SFLPKK177 pKa = 10.29 SKK179 pKa = 10.72 SGFVSPYY186 pKa = 10.17 YY187 pKa = 10.86 LDD189 pKa = 3.85 VQQLASLQADD199 pKa = 3.87 YY200 pKa = 11.25 PEE202 pKa = 5.67 LGVSGGGTIHH212 pKa = 6.99 HH213 pKa = 6.55 PHH215 pKa = 7.18 AYY217 pKa = 9.93 AAVATLCQEE226 pKa = 4.44 AIILKK231 pKa = 9.71 RR232 pKa = 11.84 MNYY235 pKa = 10.07 GSTTVPPRR243 pKa = 11.84 GYY245 pKa = 9.98 DD246 pKa = 2.91 AYY248 pKa = 10.68 AIDD251 pKa = 3.82 VGANYY256 pKa = 8.64 PRR258 pKa = 11.84 HH259 pKa = 5.82 AKK261 pKa = 10.11 AGRR264 pKa = 11.84 FDD266 pKa = 3.47 VHH268 pKa = 6.24 NCCPIITGRR277 pKa = 11.84 DD278 pKa = 3.24 SARR281 pKa = 11.84 DD282 pKa = 3.52 TTRR285 pKa = 11.84 ALVMASLVSGGKK297 pKa = 8.03 LTAEE301 pKa = 4.21 TALKK305 pKa = 8.84 YY306 pKa = 9.12 TDD308 pKa = 3.93 PASVPGKK315 pKa = 10.34 NLLRR319 pKa = 11.84 CANRR323 pKa = 11.84 AEE325 pKa = 4.35 QCTVTAPYY333 pKa = 10.51 MMFVHH338 pKa = 6.06 SQYY341 pKa = 11.28 DD342 pKa = 3.23 ITLEE346 pKa = 3.74 QLGAMFDD353 pKa = 3.26 AHH355 pKa = 5.92 RR356 pKa = 11.84 TIKK359 pKa = 10.36 AYY361 pKa = 10.83 SVMNFVPDD369 pKa = 3.72 IFVASQGTVASLGLTYY385 pKa = 10.36 HH386 pKa = 7.0 KK387 pKa = 10.8 EE388 pKa = 3.72 GDD390 pKa = 3.61 DD391 pKa = 3.63 VVFSFVGDD399 pKa = 3.1 SSMNYY404 pKa = 8.84 RR405 pKa = 11.84 HH406 pKa = 6.41 NLNTLLRR413 pKa = 11.84 TYY415 pKa = 8.24 TAQHH419 pKa = 6.92 IISPSGVMYY428 pKa = 9.67 VCEE431 pKa = 3.69 RR432 pKa = 11.84 VVRR435 pKa = 11.84 GGALFVEE442 pKa = 5.1 YY443 pKa = 9.34 TRR445 pKa = 11.84 CTMAPEE451 pKa = 3.94 LQEE454 pKa = 4.42 EE455 pKa = 4.71 YY456 pKa = 11.0 FDD458 pKa = 5.18 FNFWDD463 pKa = 3.77 AAAEE467 pKa = 4.0 DD468 pKa = 4.35 VVYY471 pKa = 9.26 LTTWQYY477 pKa = 12.0 DD478 pKa = 3.34 HH479 pKa = 6.6 TRR481 pKa = 11.84 YY482 pKa = 10.18 AGSKK486 pKa = 8.73 MAVKK490 pKa = 10.52 GPAGFHH496 pKa = 6.1 RR497 pKa = 11.84 LIIGVDD503 pKa = 2.82 RR504 pKa = 11.84 AFFEE508 pKa = 4.28 RR509 pKa = 11.84 VYY511 pKa = 9.45 VHH513 pKa = 6.7 CLRR516 pKa = 11.84 SGDD519 pKa = 3.25 TSFIINHH526 pKa = 5.23 VVSAASTFNTKK537 pKa = 9.15 MSINGANIRR546 pKa = 11.84 AVNRR550 pKa = 11.84 VGMDD554 pKa = 3.09 VFFDD558 pKa = 3.79 AVIAIYY564 pKa = 9.25 MIAYY568 pKa = 6.31 RR569 pKa = 11.84 TRR571 pKa = 11.84 WMGTKK576 pKa = 8.76 TLHH579 pKa = 6.71 HH580 pKa = 6.7 MIEE583 pKa = 4.26 SEE585 pKa = 3.74 KK586 pKa = 10.75 GVRR589 pKa = 11.84 GDD591 pKa = 3.65 YY592 pKa = 10.77 KK593 pKa = 10.57 IGFIGAFKK601 pKa = 10.69 ALWKK605 pKa = 9.98 ILSSFTTGKK614 pKa = 10.14 LFEE617 pKa = 5.56 SIAEE621 pKa = 3.99 VWNKK625 pKa = 9.26 FVSKK629 pKa = 10.6 CGEE632 pKa = 4.06 LNSAGDD638 pKa = 4.03 LDD640 pKa = 4.6 VEE642 pKa = 4.47 LLQSVRR648 pKa = 11.84 QIHH651 pKa = 6.23 FSDD654 pKa = 3.98 LLRR657 pKa = 11.84 YY658 pKa = 7.58 NAKK661 pKa = 10.72 YY662 pKa = 9.99 EE663 pKa = 4.1 GAYY666 pKa = 8.73 PALYY670 pKa = 10.01 DD671 pKa = 4.09 IRR673 pKa = 11.84 VDD675 pKa = 4.32 AIKK678 pKa = 10.59 FDD680 pKa = 4.07 VKK682 pKa = 11.05 DD683 pKa = 3.82 GMLQAISEE691 pKa = 4.66 VVRR694 pKa = 11.84 SKK696 pKa = 11.21 YY697 pKa = 10.74 SSDD700 pKa = 3.31 PADD703 pKa = 3.82 PPKK706 pKa = 10.7 RR707 pKa = 11.84 PAHH710 pKa = 6.5 VDD712 pKa = 3.51 GQHH715 pKa = 6.64 GNDD718 pKa = 3.98 HH719 pKa = 7.16 KK720 pKa = 11.53 DD721 pKa = 3.24 PGNVTGNTDD730 pKa = 3.46 TPKK733 pKa = 10.63 EE734 pKa = 4.24 SPQPGLPPAPSAPRR748 pKa = 11.84 VVGTPAAPMSNKK760 pKa = 8.19 TRR762 pKa = 11.84 NGVITEE768 pKa = 4.16 FHH770 pKa = 6.23 RR771 pKa = 11.84 TQSIKK776 pKa = 10.36 SEE778 pKa = 4.3 KK779 pKa = 9.83 NSSRR783 pKa = 11.84 LRR785 pKa = 11.84 AGRR788 pKa = 11.84 HH789 pKa = 4.07 EE790 pKa = 5.33 AYY792 pKa = 10.2 ACTSVLVVKK801 pKa = 7.48 PTPPDD806 pKa = 3.77 GNCLFTSLGYY816 pKa = 9.72 FVNLTANEE824 pKa = 4.0 MRR826 pKa = 11.84 ADD828 pKa = 3.45 LCGAVGDD835 pKa = 4.0 MEE837 pKa = 5.62 LRR839 pKa = 11.84 YY840 pKa = 9.97 NDD842 pKa = 3.92 RR843 pKa = 11.84 GRR845 pKa = 11.84 STWGNTDD852 pKa = 3.12 CVTTFSSLYY861 pKa = 8.07 QKK863 pKa = 10.17 RR864 pKa = 11.84 VCVHH868 pKa = 4.96 MTVTDD873 pKa = 3.38 KK874 pKa = 10.52 KK875 pKa = 8.85 TYY877 pKa = 7.7 PTVTSEE883 pKa = 4.13 MYY885 pKa = 10.23 SAEE888 pKa = 3.92 GTTDD892 pKa = 3.22 MVHH895 pKa = 6.77 LDD897 pKa = 3.21 WRR899 pKa = 11.84 VVGDD903 pKa = 3.94 PKK905 pKa = 10.85 TNNHH909 pKa = 5.71 TGHH912 pKa = 6.71 VEE914 pKa = 4.27 VLTQPNDD921 pKa = 3.11 KK922 pKa = 10.82 RR923 pKa = 11.84 EE924 pKa = 4.24 LPRR927 pKa = 11.84 EE928 pKa = 4.08 APTWKK933 pKa = 10.19 SVSHH937 pKa = 6.75 LFDD940 pKa = 3.04 TTRR943 pKa = 11.84 RR944 pKa = 11.84 LPHH947 pKa = 6.53 GSTDD951 pKa = 3.49 EE952 pKa = 3.93 YY953 pKa = 11.09 HH954 pKa = 6.67 GFPRR958 pKa = 11.84 HH959 pKa = 5.52 QEE961 pKa = 3.86 LKK963 pKa = 10.57 ALSLLGSADD972 pKa = 3.47 AATTRR977 pKa = 11.84 PASILEE983 pKa = 4.18 LGAAPGTWTKK993 pKa = 11.2 LLLTYY998 pKa = 10.85 ASARR1002 pKa = 11.84 RR1003 pKa = 11.84 AAIDD1007 pKa = 3.7 VVSAPTGLEE1016 pKa = 3.65 MAEE1019 pKa = 3.96 EE1020 pKa = 4.19 VLAEE1024 pKa = 4.12 IEE1026 pKa = 4.34 SDD1028 pKa = 3.82 DD1029 pKa = 5.47 AYY1031 pKa = 10.15 TLHH1034 pKa = 6.06 QQDD1037 pKa = 3.69 AVTFLTEE1044 pKa = 3.78 TAKK1047 pKa = 10.82 KK1048 pKa = 10.07 YY1049 pKa = 11.09 DD1050 pKa = 3.66 FVLSDD1055 pKa = 3.42 VATADD1060 pKa = 2.99 SWATNSPQLDD1070 pKa = 3.66 VLCAVVSRR1078 pKa = 11.84 LNLGATLVLKK1088 pKa = 10.59 LSNVFLDD1095 pKa = 3.59 NSLDD1099 pKa = 4.0 AIANCRR1105 pKa = 11.84 RR1106 pKa = 11.84 LFASVEE1112 pKa = 4.27 VVKK1115 pKa = 10.1 PAGSRR1120 pKa = 11.84 LRR1122 pKa = 11.84 NTEE1125 pKa = 3.67 VYY1127 pKa = 9.59 LVCKK1131 pKa = 10.33 AYY1133 pKa = 10.48 GEE1135 pKa = 4.29 PAGRR1139 pKa = 11.84 TNPYY1143 pKa = 7.83 SQRR1146 pKa = 11.84 RR1147 pKa = 11.84 LIIADD1152 pKa = 3.47 ILAHH1156 pKa = 6.28 LEE1158 pKa = 3.83 RR1159 pKa = 11.84 LKK1161 pKa = 11.14 AGEE1164 pKa = 4.15 TVVRR1168 pKa = 11.84 DD1169 pKa = 3.7 VVDD1172 pKa = 4.1 EE1173 pKa = 3.94 YY1174 pKa = 11.86 SRR1176 pKa = 11.84 LSFAFIEE1183 pKa = 4.35 YY1184 pKa = 9.32 TRR1186 pKa = 11.84 DD1187 pKa = 3.03 VTMEE1191 pKa = 3.85 WDD1193 pKa = 3.84 DD1194 pKa = 4.12 PSDD1197 pKa = 4.2 GEE1199 pKa = 4.69 EE1200 pKa = 4.21 EE1201 pKa = 4.35 SQADD1205 pKa = 4.28 PVPPATPALTPVQEE1219 pKa = 4.86 LYY1221 pKa = 10.65 TLTFVEE1227 pKa = 4.39 AQIAAAASRR1236 pKa = 11.84 LAEE1239 pKa = 4.27 DD1240 pKa = 3.53 TGRR1243 pKa = 11.84 PASSACARR1251 pKa = 11.84 PATPTPSCAPSDD1263 pKa = 4.19 FSTKK1267 pKa = 9.86 SAPAAIPTPDD1277 pKa = 3.72 SPKK1280 pKa = 10.51 LNPPSTMPNAAGSSPPEE1297 pKa = 3.84 PTPARR1302 pKa = 11.84 SSPTSSCASSDD1313 pKa = 3.2 AGVEE1317 pKa = 4.56 TTCPPRR1323 pKa = 11.84 PNPPCTLPTAAGSSGPEE1340 pKa = 3.64 TTPARR1345 pKa = 11.84 GSAEE1349 pKa = 3.61 RR1350 pKa = 11.84 PTRR1353 pKa = 11.84 SKK1355 pKa = 10.95 RR1356 pKa = 11.84 PIPSFVFTPSVKK1368 pKa = 9.78 TSLEE1372 pKa = 4.16 SQPSLLSIGEE1382 pKa = 4.1 NAKK1385 pKa = 10.28 VEE1387 pKa = 3.98 ASTTLGANASPALSSTFLAEE1407 pKa = 4.16 NGLVMNLDD1415 pKa = 3.82 DD1416 pKa = 4.94 ALFDD1420 pKa = 5.54 AEE1422 pKa = 5.38 HH1423 pKa = 6.93 PWQSAAHH1430 pKa = 5.96 HH1431 pKa = 5.54 MQVYY1435 pKa = 10.18 HH1436 pKa = 6.81 KK1437 pKa = 11.01 GSGLYY1442 pKa = 9.65 EE1443 pKa = 5.01 RR1444 pKa = 11.84 IMEE1447 pKa = 4.51 LGVYY1451 pKa = 9.52 SDD1453 pKa = 3.92 RR1454 pKa = 11.84 LHH1456 pKa = 7.15 YY1457 pKa = 10.38 EE1458 pKa = 3.46 YY1459 pKa = 11.13 DD1460 pKa = 3.75 EE1461 pKa = 4.4 IMSLLGPTACDD1472 pKa = 3.25 FALPEE1477 pKa = 4.09 PVALLVQPEE1486 pKa = 4.24 GAACLTKK1493 pKa = 10.54 AHH1495 pKa = 6.17 SASRR1499 pKa = 11.84 LADD1502 pKa = 3.51 GKK1504 pKa = 8.45 TVAVRR1509 pKa = 11.84 VSDD1512 pKa = 3.68 PGVPHH1517 pKa = 6.47 LTAPGWHH1524 pKa = 5.66 VQVHH1528 pKa = 5.94 RR1529 pKa = 11.84 CRR1531 pKa = 11.84 STPPTDD1537 pKa = 3.06 VAVYY1541 pKa = 9.12 YY1542 pKa = 9.93 IYY1544 pKa = 10.78 SQLPSRR1550 pKa = 11.84 VVAGSWKK1557 pKa = 8.84 HH1558 pKa = 5.9 TMSQCLNAAVKK1569 pKa = 10.38 GVAPYY1574 pKa = 10.84 DD1575 pKa = 3.7 PALLRR1580 pKa = 11.84 AVLRR1584 pKa = 11.84 TTAAGGCLSDD1594 pKa = 4.89 LEE1596 pKa = 4.92 AVSKK1600 pKa = 10.94 CDD1602 pKa = 3.72 AHH1604 pKa = 6.34 TEE1606 pKa = 3.81 LDD1608 pKa = 4.29 KK1609 pKa = 11.69 ACLCCVYY1616 pKa = 9.51 VTSGDD1621 pKa = 3.37 IQPPMVASWMRR1632 pKa = 11.84 RR1633 pKa = 11.84 KK1634 pKa = 10.08 YY1635 pKa = 10.44 NQIAARR1641 pKa = 11.84 AKK1643 pKa = 10.21 RR1644 pKa = 11.84 IYY1646 pKa = 10.34 LEE1648 pKa = 4.26 NGGAEE1653 pKa = 4.07 ALSLDD1658 pKa = 3.91 EE1659 pKa = 4.9 ALPVYY1664 pKa = 7.32 MQRR1667 pKa = 11.84 EE1668 pKa = 3.99 RR1669 pKa = 11.84 RR1670 pKa = 11.84 DD1671 pKa = 3.09 ISASGLRR1678 pKa = 11.84 VDD1680 pKa = 5.29 GSAITLKK1687 pKa = 10.64 SYY1689 pKa = 10.41 EE1690 pKa = 4.14 PNKK1693 pKa = 10.04 FIPAFTTGGQVINAMKK1709 pKa = 10.26 PGTHH1713 pKa = 6.17 KK1714 pKa = 10.83 VSAGDD1719 pKa = 3.48 QVAHH1723 pKa = 4.99 VTTYY1727 pKa = 11.19 GAVAKK1732 pKa = 8.65 QTVDD1736 pKa = 3.52 FWGSKK1741 pKa = 8.76 GRR1743 pKa = 11.84 TRR1745 pKa = 11.84 AAVTDD1750 pKa = 3.47 AKK1752 pKa = 10.82 GRR1754 pKa = 11.84 HH1755 pKa = 4.27 TTLSEE1760 pKa = 4.2 VVGTSSRR1767 pKa = 11.84 VTSTVTNVASLPAPEE1782 pKa = 4.32 NRR1784 pKa = 11.84 TVRR1787 pKa = 11.84 DD1788 pKa = 3.43 VAVSVSDD1795 pKa = 3.81 AKK1797 pKa = 10.63 FGCVSVHH1804 pKa = 6.18 ASIPRR1809 pKa = 11.84 AANVVVCQKK1818 pKa = 11.01 LITYY1822 pKa = 7.7 KK1823 pKa = 10.31 TAGTQTAEE1831 pKa = 4.28 TGSAAYY1837 pKa = 7.45 TICAKK1842 pKa = 9.3 KK1843 pKa = 9.14 TSPDD1847 pKa = 3.4 LPSASEE1853 pKa = 4.12 SDD1855 pKa = 3.59 TTTLTSTTSTSVSITKK1871 pKa = 10.38 RR1872 pKa = 11.84 GDD1874 pKa = 3.36 DD1875 pKa = 3.8 FTSTTSTPTGISQFTEE1891 pKa = 3.9 TGPVAFTAALKK1902 pKa = 10.54 ARR1904 pKa = 11.84 SEE1906 pKa = 4.07 AVQRR1910 pKa = 11.84 AKK1912 pKa = 10.78 RR1913 pKa = 11.84 EE1914 pKa = 4.14 AEE1916 pKa = 4.08 TFQPLATSSTISEE1929 pKa = 4.08 SDD1931 pKa = 2.97 ASKK1934 pKa = 10.85 SVSTTGTGVTVPFASGGKK1952 pKa = 8.45 PASDD1956 pKa = 4.29 TSNSWAPSSTVSSSSTSTSASTVGVGASTSSSSGKK1991 pKa = 9.55 KK1992 pKa = 8.83 HH1993 pKa = 6.48 ASRR1996 pKa = 11.84 TASLMAKK2003 pKa = 10.24 LGLDD2007 pKa = 3.89 DD2008 pKa = 5.36 ASSASTEE2015 pKa = 4.11 VASAGQASTNALLPDD2030 pKa = 3.19 KK2031 pKa = 10.22 TKK2033 pKa = 10.63 RR2034 pKa = 11.84 SKK2036 pKa = 10.92 LQACKK2041 pKa = 10.34 SAFKK2045 pKa = 10.95 AVMQPSKK2052 pKa = 10.7 AATPRR2057 pKa = 11.84 QPAAEE2062 pKa = 4.38 VCQSLPRR2069 pKa = 11.84 GCKK2072 pKa = 8.94 DD2073 pKa = 2.99 TLAVGGTWTGYY2084 pKa = 9.53 PHH2086 pKa = 7.47 DD2087 pKa = 4.97 CKK2089 pKa = 10.92 LVLHH2093 pKa = 6.78 LLLSCGKK2100 pKa = 9.77 EE2101 pKa = 4.03 FLATDD2106 pKa = 4.08 SLAKK2110 pKa = 10.07 RR2111 pKa = 11.84 LKK2113 pKa = 10.14 KK2114 pKa = 10.43 EE2115 pKa = 4.41 RR2116 pKa = 11.84 ITEE2119 pKa = 3.79 CDD2121 pKa = 3.08 VARR2124 pKa = 11.84 RR2125 pKa = 11.84 GYY2127 pKa = 10.71 SRR2129 pKa = 11.84 LEE2131 pKa = 3.64 HH2132 pKa = 5.7 TLEE2135 pKa = 4.56 LFYY2138 pKa = 11.13 HH2139 pKa = 6.95 HH2140 pKa = 7.67 DD2141 pKa = 3.81 GVHH2144 pKa = 6.68 DD2145 pKa = 4.47 FVLVTPAVGAEE2156 pKa = 4.07 GVLKK2160 pKa = 10.68 RR2161 pKa = 11.84 FLSWLPEE2168 pKa = 4.13 DD2169 pKa = 4.01 VSRR2172 pKa = 11.84 VAIEE2176 pKa = 4.2 SSADD2180 pKa = 3.56 LADD2183 pKa = 3.84 SALTEE2188 pKa = 4.29 LLRR2191 pKa = 11.84 EE2192 pKa = 4.16 CDD2194 pKa = 3.49 RR2195 pKa = 11.84 PVTLVDD2201 pKa = 2.93 GRR2203 pKa = 11.84 TRR2205 pKa = 11.84 RR2206 pKa = 11.84 WAGVLHH2212 pKa = 6.57 LLEE2215 pKa = 4.4 PSKK2218 pKa = 10.97 RR2219 pKa = 11.84 LLPPVRR2225 pKa = 11.84 RR2226 pKa = 11.84 PLAFPKK2232 pKa = 10.47 LSQVEE2237 pKa = 4.01 MGYY2240 pKa = 9.86 IPNPPWIAANTNLDD2254 pKa = 3.54 RR2255 pKa = 11.84 YY2256 pKa = 8.54 RR2257 pKa = 11.84 NAMIEE2262 pKa = 3.94 YY2263 pKa = 9.74 RR2264 pKa = 11.84 YY2265 pKa = 6.31 TTAHH2269 pKa = 6.85 ADD2271 pKa = 3.53 QFNKK2275 pKa = 10.41 LHH2277 pKa = 5.5 FQKK2280 pKa = 10.88 LQLDD2284 pKa = 4.29 SKK2286 pKa = 11.3 NGIPAGKK2293 pKa = 7.74 TAEE2296 pKa = 4.16 ILAAGYY2302 pKa = 9.86 GVYY2305 pKa = 10.19 DD2306 pKa = 3.93 QQTKK2310 pKa = 10.72 AYY2312 pKa = 8.89 LGQKK2316 pKa = 10.12 ASRR2319 pKa = 11.84 TYY2321 pKa = 9.0 SHH2323 pKa = 7.2 GYY2325 pKa = 8.98 SVSKK2329 pKa = 10.46 QEE2331 pKa = 3.72 YY2332 pKa = 7.93 VAWNEE2337 pKa = 3.65 TARR2340 pKa = 11.84 QFNSPDD2346 pKa = 3.03 RR2347 pKa = 11.84 FILVGRR2353 pKa = 11.84 NTEE2356 pKa = 4.17 LMLNSQISAQIAGVDD2371 pKa = 3.76 VASQEE2376 pKa = 4.04 LPEE2379 pKa = 4.51 IEE2381 pKa = 4.95 WINGPPGCGKK2391 pKa = 7.85 THH2393 pKa = 7.55 AIVHH2397 pKa = 5.65 SANVSIDD2404 pKa = 3.58 PLVGRR2409 pKa = 11.84 DD2410 pKa = 4.5 LILSMTSEE2418 pKa = 4.37 GKK2420 pKa = 8.54 TSIRR2424 pKa = 11.84 AGLKK2428 pKa = 9.83 KK2429 pKa = 10.25 RR2430 pKa = 11.84 LPSLTDD2436 pKa = 3.06 RR2437 pKa = 11.84 ALQAHH2442 pKa = 5.73 VRR2444 pKa = 11.84 TVASLLVNGSAVKK2457 pKa = 10.26 YY2458 pKa = 10.53 DD2459 pKa = 3.49 RR2460 pKa = 11.84 VLMDD2464 pKa = 3.84 EE2465 pKa = 4.89 ALMAHH2470 pKa = 7.37 AGTIGFAVALTGTKK2484 pKa = 9.76 KK2485 pKa = 10.72 VLIIGDD2491 pKa = 3.67 IHH2493 pKa = 6.75 QIPYY2497 pKa = 9.87 VDD2499 pKa = 4.06 RR2500 pKa = 11.84 EE2501 pKa = 4.45 HH2502 pKa = 6.1 MCKK2505 pKa = 10.08 LQYY2508 pKa = 7.43 EE2509 pKa = 4.76 TPAVFADD2516 pKa = 3.56 VTSVKK2521 pKa = 10.07 EE2522 pKa = 3.61 ITYY2525 pKa = 9.4 RR2526 pKa = 11.84 CPMDD2530 pKa = 3.28 VTYY2533 pKa = 10.32 AISDD2537 pKa = 4.41 LYY2539 pKa = 10.76 PNLCTMSLVTVSVNQKK2555 pKa = 9.06 PWSSNNTHH2563 pKa = 4.46 VTKK2566 pKa = 10.14 TVEE2569 pKa = 3.76 GCLYY2573 pKa = 9.35 LTHH2576 pKa = 6.54 TQPDD2580 pKa = 3.42 KK2581 pKa = 11.34 DD2582 pKa = 3.84 ALIKK2586 pKa = 10.68 AGYY2589 pKa = 9.51 GKK2591 pKa = 10.74 GAGSAVMTIHH2601 pKa = 6.87 EE2602 pKa = 4.56 AQGLTYY2608 pKa = 10.86 AHH2610 pKa = 5.8 VVCIRR2615 pKa = 11.84 SQPKK2619 pKa = 9.84 ALAIYY2624 pKa = 10.03 SRR2626 pKa = 11.84 SEE2628 pKa = 3.9 YY2629 pKa = 11.15 ALVAISRR2636 pKa = 11.84 HH2637 pKa = 4.64 TKK2639 pKa = 9.66 SFVYY2643 pKa = 9.37 YY2644 pKa = 10.22 TDD2646 pKa = 3.57 VDD2648 pKa = 3.87 DD2649 pKa = 6.44 ALTKK2653 pKa = 10.58 LIRR2656 pKa = 11.84 KK2657 pKa = 7.71 AQSKK2661 pKa = 10.82 DD2662 pKa = 3.13 PAALQIWNAPRR2673 pKa = 11.84 LTAAKK2678 pKa = 10.07 KK2679 pKa = 10.48 KK2680 pKa = 8.39 LTPLTAGGLLWEE2692 pKa = 5.27 ADD2694 pKa = 3.78 TVLSPLANPTTGDD2707 pKa = 3.5 YY2708 pKa = 9.66 MAGLPEE2714 pKa = 4.38 VSHH2717 pKa = 6.7 APLMPLAPEE2726 pKa = 5.32 RR2727 pKa = 11.84 IAEE2730 pKa = 4.1 SSFRR2734 pKa = 11.84 KK2735 pKa = 9.6 LPKK2738 pKa = 9.79 FRR2740 pKa = 11.84 PNVDD2744 pKa = 2.63 TDD2746 pKa = 3.66 VAFLQTFYY2754 pKa = 11.31 DD2755 pKa = 3.82 DD2756 pKa = 4.49 VMAEE2760 pKa = 4.65 AITVEE2765 pKa = 4.05 YY2766 pKa = 10.32 KK2767 pKa = 10.05 YY2768 pKa = 10.77 DD2769 pKa = 3.43 QMMMEE2774 pKa = 4.5 MEE2776 pKa = 4.17 DD2777 pKa = 3.81 TYY2779 pKa = 11.51 LVSTPITVDD2788 pKa = 3.36 PLLGLPADD2796 pKa = 3.97 RR2797 pKa = 11.84 KK2798 pKa = 9.45 YY2799 pKa = 11.52 GKK2801 pKa = 9.82 LRR2803 pKa = 11.84 PLLRR2807 pKa = 11.84 TNMRR2811 pKa = 11.84 AEE2813 pKa = 4.01 KK2814 pKa = 10.32 QPSQKK2819 pKa = 10.39 EE2820 pKa = 4.25 SLLGAIKK2827 pKa = 10.6 RR2828 pKa = 11.84 NLNAPLLRR2836 pKa = 11.84 NAALSEE2842 pKa = 4.04 EE2843 pKa = 4.81 AIGEE2847 pKa = 4.17 LLFKK2851 pKa = 10.8 NFEE2854 pKa = 3.93 RR2855 pKa = 11.84 SAIDD2859 pKa = 3.35 EE2860 pKa = 4.53 TKK2862 pKa = 9.48 VAIYY2866 pKa = 9.9 EE2867 pKa = 4.37 SYY2869 pKa = 11.48 ADD2871 pKa = 4.55 DD2872 pKa = 5.43 PININSQVVSVWLEE2886 pKa = 3.62 KK2887 pKa = 10.51 QPPSRR2892 pKa = 11.84 RR2893 pKa = 11.84 KK2894 pKa = 10.1 QIVSDD2899 pKa = 4.21 LPLHH2903 pKa = 6.43 LRR2905 pKa = 11.84 PFNKK2909 pKa = 9.89 FEE2911 pKa = 4.16 FMVKK2915 pKa = 9.93 KK2916 pKa = 10.22 DD2917 pKa = 3.93 VKK2919 pKa = 10.5 PQLTPDD2925 pKa = 3.1 SMYY2928 pKa = 10.57 SYY2930 pKa = 10.47 PSVQTIVYY2938 pKa = 10.26 NDD2940 pKa = 3.43 ASVNAVSCPIYY2951 pKa = 10.22 NLLWEE2956 pKa = 4.61 RR2957 pKa = 11.84 LLAVLDD2963 pKa = 3.83 PRR2965 pKa = 11.84 VLVMTGMSPVEE2976 pKa = 4.43 FEE2978 pKa = 4.76 AEE2980 pKa = 4.04 FNARR2984 pKa = 11.84 LCPEE2988 pKa = 4.03 VASVLRR2994 pKa = 11.84 TLEE2997 pKa = 4.04 NDD2999 pKa = 2.89 FSKK3002 pKa = 11.12 YY3003 pKa = 10.81 DD3004 pKa = 3.49 KK3005 pKa = 10.93 AQAGALRR3012 pKa = 11.84 RR3013 pKa = 11.84 LEE3015 pKa = 3.97 HH3016 pKa = 6.77 LVWTKK3021 pKa = 11.03 LGLDD3025 pKa = 3.86 PEE3027 pKa = 4.65 IALIWDD3033 pKa = 3.96 RR3034 pKa = 11.84 SRR3036 pKa = 11.84 RR3037 pKa = 11.84 TSDD3040 pKa = 2.88 VRR3042 pKa = 11.84 DD3043 pKa = 3.54 RR3044 pKa = 11.84 KK3045 pKa = 10.65 NGVSFVTEE3053 pKa = 3.88 YY3054 pKa = 10.45 QRR3056 pKa = 11.84 KK3057 pKa = 9.09 SGEE3060 pKa = 3.85 ATTFSGNTLVAMCVMLAVIDD3080 pKa = 3.82 IDD3082 pKa = 5.96 DD3083 pKa = 4.1 IVLMLAAGDD3092 pKa = 3.72 DD3093 pKa = 3.65 TLIYY3097 pKa = 10.07 LRR3099 pKa = 11.84 PGTDD3103 pKa = 3.74 FSDD3106 pKa = 3.85 SSALVADD3113 pKa = 5.16 LFNLEE3118 pKa = 4.5 CKK3120 pKa = 10.43 LLEE3123 pKa = 4.73 CYY3125 pKa = 8.02 EE3126 pKa = 4.13 VPYY3129 pKa = 10.43 FCSKK3133 pKa = 10.61 FLISTPDD3140 pKa = 2.67 WTYY3143 pKa = 10.58 FVHH3146 pKa = 7.74 DD3147 pKa = 4.45 LLKK3150 pKa = 10.65 FVTKK3154 pKa = 10.83 LGRR3157 pKa = 11.84 HH3158 pKa = 5.82 DD3159 pKa = 3.61 MSNYY3163 pKa = 7.77 THH3165 pKa = 6.29 VEE3167 pKa = 3.99 NYY3169 pKa = 9.7 RR3170 pKa = 11.84 VSCVDD3175 pKa = 2.97 TMRR3178 pKa = 11.84 SLFNPVVAPGLTVGMQEE3195 pKa = 4.59 RR3196 pKa = 11.84 YY3197 pKa = 9.73 HH3198 pKa = 6.36 STFCDD3203 pKa = 3.51 VTKK3206 pKa = 10.93 VLAVLRR3212 pKa = 11.84 TLCHH3216 pKa = 6.57 DD3217 pKa = 3.98 PKK3219 pKa = 10.98 KK3220 pKa = 10.35 FAALYY3225 pKa = 9.79 LHH3227 pKa = 7.29 DD3228 pKa = 5.57 KK3229 pKa = 11.12 GVILCNDD3236 pKa = 3.9 PSASRR3241 pKa = 11.84 LHH3243 pKa = 6.21
Molecular weight: 354.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.716
IPC2_protein 6.971
IPC_protein 6.839
Toseland 6.781
ProMoST 7.395
Dawson 7.439
Bjellqvist 7.805
Wikipedia 7.38
Rodwell 7.468
Grimsley 6.883
Solomon 7.454
Lehninger 7.468
Nozaki 8.039
DTASelect 7.702
Thurlkill 7.746
EMBOSS 7.732
Sillero 7.966
Patrickios 4.202
IPC_peptide 7.454
IPC2_peptide 7.205
IPC2.peptide.svr19 6.969
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3448
205
3243
1724.0
188.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.557 ± 0.53
1.885 ± 0.03
5.8 ± 0.698
4.843 ± 0.016
3.335 ± 1.609
5.423 ± 0.925
2.61 ± 0.595
3.393 ± 0.888
4.814 ± 1.318
8.817 ± 0.69
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.088 ± 0.288
3.335 ± 1.609
5.916 ± 0.196
2.726 ± 0.132
5.829 ± 0.909
8.353 ± 0.926
7.831 ± 0.91
7.976 ± 0.146
1.044 ± 0.418
3.422 ± 0.677
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here