Bifidobacterium felsineum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9HLG2|A0A2M9HLG2_9BIFI Sucrose phosphorylase OS=Bifidobacterium felsineum OX=2045440 GN=CSQ86_00730 PE=3 SV=1
MM1 pKa = 7.21TLEE4 pKa = 4.45NSDD7 pKa = 3.49AAQALVQEE15 pKa = 4.54IVRR18 pKa = 11.84RR19 pKa = 11.84IVRR22 pKa = 11.84DD23 pKa = 3.49TSKK26 pKa = 9.95MAVSFTPEE34 pKa = 3.72LDD36 pKa = 3.37VRR38 pKa = 11.84PDD40 pKa = 3.35LLGNFIGGDD49 pKa = 4.28SYY51 pKa = 11.23WSPQGVPVTSADD63 pKa = 3.51GSPLYY68 pKa = 10.21PLMQIRR74 pKa = 11.84CTEE77 pKa = 4.07PSLPGYY83 pKa = 6.29PTRR86 pKa = 11.84GLLQFFIGTDD96 pKa = 2.84IMYY99 pKa = 10.36GCDD102 pKa = 3.49LSADD106 pKa = 3.91YY107 pKa = 10.96DD108 pKa = 4.17SEE110 pKa = 5.21ADD112 pKa = 3.31NDD114 pKa = 3.97NRR116 pKa = 11.84CVRR119 pKa = 11.84YY120 pKa = 9.56IPEE123 pKa = 4.18VPSVLSGAEE132 pKa = 4.07IVRR135 pKa = 11.84LPHH138 pKa = 6.86IGADD142 pKa = 3.67SDD144 pKa = 4.48PDD146 pKa = 3.22LHH148 pKa = 6.6TPFRR152 pKa = 11.84SNFHH156 pKa = 5.86GCALRR161 pKa = 11.84PALIEE166 pKa = 4.54DD167 pKa = 4.39PVSHH171 pKa = 6.64EE172 pKa = 4.1CDD174 pKa = 3.31EE175 pKa = 4.6FEE177 pKa = 4.37PAYY180 pKa = 10.12EE181 pKa = 4.15SIIAEE186 pKa = 4.31FDD188 pKa = 3.56PAVIEE193 pKa = 4.13ATEE196 pKa = 4.17DD197 pKa = 3.37AVGDD201 pKa = 4.03LDD203 pKa = 4.61QAVMDD208 pKa = 4.12QLNAVSPGSWFQMGGHH224 pKa = 6.59PVFTQSDD231 pKa = 3.57VRR233 pKa = 11.84EE234 pKa = 4.65DD235 pKa = 3.88YY236 pKa = 11.25DD237 pKa = 5.59DD238 pKa = 4.39EE239 pKa = 5.25LVLLKK244 pKa = 10.4VDD246 pKa = 4.07SYY248 pKa = 12.04DD249 pKa = 4.69DD250 pKa = 3.47VLMWGDD256 pKa = 3.61SGCANFLIRR265 pKa = 11.84RR266 pKa = 11.84EE267 pKa = 4.18DD268 pKa = 3.66LEE270 pKa = 4.04QLDD273 pKa = 4.64FSHH276 pKa = 7.29VLYY279 pKa = 9.67TWDD282 pKa = 3.85CLL284 pKa = 3.89

Molecular weight: 31.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9HLA6|A0A2M9HLA6_9BIFI Metallophos domain-containing protein OS=Bifidobacterium felsineum OX=2045440 GN=CSQ86_00360 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1828

0

1828

685830

29

3163

375.2

40.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.082 ± 0.078

0.861 ± 0.018

6.276 ± 0.048

5.668 ± 0.052

3.594 ± 0.033

7.878 ± 0.046

2.192 ± 0.03

5.665 ± 0.041

4.228 ± 0.047

8.855 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.028

3.638 ± 0.044

4.556 ± 0.036

3.522 ± 0.036

5.246 ± 0.057

6.092 ± 0.051

6.188 ± 0.052

7.699 ± 0.05

1.392 ± 0.025

2.823 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski