SAR202 cluster bacterium AD-802-E10_MRT_200m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 589 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5N8Z6J3|A0A5N8Z6J3_9CHLR Vitamin B12-dependent ribonucleotide reductase OS=SAR202 cluster bacterium AD-802-E10_MRT_200m OX=2587840 GN=FIM02_02625 PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 9.98AAKK5 pKa = 10.28VNEE8 pKa = 4.09LQPGEE13 pKa = 4.03IKK15 pKa = 10.74YY16 pKa = 10.61VEE18 pKa = 4.27LNDD21 pKa = 4.78VEE23 pKa = 4.87ILLANIDD30 pKa = 3.78GEE32 pKa = 4.56LYY34 pKa = 10.7AVSDD38 pKa = 4.4TCTHH42 pKa = 7.05AYY44 pKa = 10.5ASLSDD49 pKa = 3.96GALEE53 pKa = 4.75GCQISCPIHH62 pKa = 6.68GGVFDD67 pKa = 4.75VITGEE72 pKa = 4.45VIQGPPDD79 pKa = 3.37EE80 pKa = 4.57ALEE83 pKa = 4.37RR84 pKa = 11.84YY85 pKa = 8.76AVKK88 pKa = 10.97LEE90 pKa = 4.29GDD92 pKa = 4.85DD93 pKa = 3.87ILIGPIEE100 pKa = 4.29NPQQ103 pKa = 2.98

Molecular weight:
11.01 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5N8Z5W4|A0A5N8Z5W4_9CHLR Coenzyme A biosynthesis bifunctional protein CoaBC OS=SAR202 cluster bacterium AD-802-E10_MRT_200m OX=2587840 GN=coaBC PE=3 SV=1
MM1 pKa = 7.21LHH3 pKa = 6.27VGVDD7 pKa = 3.5VIEE10 pKa = 4.33IEE12 pKa = 4.69RR13 pKa = 11.84IASAVDD19 pKa = 2.7RR20 pKa = 11.84WEE22 pKa = 3.75NRR24 pKa = 11.84FLDD27 pKa = 5.05RR28 pKa = 11.84IYY30 pKa = 10.74TPNEE34 pKa = 3.33LAFSRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84IPQLAGRR48 pKa = 11.84FAAKK52 pKa = 9.53EE53 pKa = 3.92AVMKK57 pKa = 10.93ALGTGARR64 pKa = 11.84GVSWRR69 pKa = 11.84DD70 pKa = 2.94IEE72 pKa = 4.4VTRR75 pKa = 11.84KK76 pKa = 9.99RR77 pKa = 11.84GFPPEE82 pKa = 3.89IQLHH86 pKa = 5.13GRR88 pKa = 11.84ALARR92 pKa = 11.84SRR94 pKa = 11.84ILNLEE99 pKa = 4.57RR100 pKa = 11.84IAISISHH107 pKa = 5.79SRR109 pKa = 11.84HH110 pKa = 4.72YY111 pKa = 10.46AIAMAVGEE119 pKa = 4.48SNEE122 pKa = 3.96NCC124 pKa = 3.9

Molecular weight:
14.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

589

0

589

180042

36

1474

305.7

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.81 ± 0.093

1.007 ± 0.036

4.951 ± 0.076

6.478 ± 0.094

4.033 ± 0.074

7.875 ± 0.086

2.142 ± 0.038

7.304 ± 0.087

4.116 ± 0.071

10.641 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.039

3.852 ± 0.048

4.636 ± 0.059

3.828 ± 0.051

5.414 ± 0.079

6.724 ± 0.072

5.654 ± 0.068

7.104 ± 0.081

1.208 ± 0.04

2.788 ± 0.049

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski