SAR202 cluster bacterium AD-802-E10_MRT_200m
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 589 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N8Z6J3|A0A5N8Z6J3_9CHLR Vitamin B12-dependent ribonucleotide reductase OS=SAR202 cluster bacterium AD-802-E10_MRT_200m OX=2587840 GN=FIM02_02625 PE=3 SV=1
MM1 pKa = 7.43 KK2 pKa = 9.98 AAKK5 pKa = 10.28 VNEE8 pKa = 4.09 LQPGEE13 pKa = 4.03 IKK15 pKa = 10.74 YY16 pKa = 10.61 VEE18 pKa = 4.27 LNDD21 pKa = 4.78 VEE23 pKa = 4.87 ILLANIDD30 pKa = 3.78 GEE32 pKa = 4.56 LYY34 pKa = 10.7 AVSDD38 pKa = 4.4 TCTHH42 pKa = 7.05 AYY44 pKa = 10.5 ASLSDD49 pKa = 3.96 GALEE53 pKa = 4.75 GCQISCPIHH62 pKa = 6.68 GGVFDD67 pKa = 4.75 VITGEE72 pKa = 4.45 VIQGPPDD79 pKa = 3.37 EE80 pKa = 4.57 ALEE83 pKa = 4.37 RR84 pKa = 11.84 YY85 pKa = 8.76 AVKK88 pKa = 10.97 LEE90 pKa = 4.29 GDD92 pKa = 4.85 DD93 pKa = 3.87 ILIGPIEE100 pKa = 4.29 NPQQ103 pKa = 2.98
Molecular weight: 11.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.801
IPC2_protein 4.024
IPC_protein 3.935
Toseland 3.757
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.795
Rodwell 3.77
Grimsley 3.668
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.177
Thurlkill 3.795
EMBOSS 3.821
Sillero 4.05
Patrickios 0.896
IPC_peptide 3.884
IPC2_peptide 4.037
IPC2.peptide.svr19 3.931
Protein with the highest isoelectric point:
>tr|A0A5N8Z5W4|A0A5N8Z5W4_9CHLR Coenzyme A biosynthesis bifunctional protein CoaBC OS=SAR202 cluster bacterium AD-802-E10_MRT_200m OX=2587840 GN=coaBC PE=3 SV=1
MM1 pKa = 7.21 LHH3 pKa = 6.27 VGVDD7 pKa = 3.5 VIEE10 pKa = 4.33 IEE12 pKa = 4.69 RR13 pKa = 11.84 IASAVDD19 pKa = 2.7 RR20 pKa = 11.84 WEE22 pKa = 3.75 NRR24 pKa = 11.84 FLDD27 pKa = 5.05 RR28 pKa = 11.84 IYY30 pKa = 10.74 TPNEE34 pKa = 3.33 LAFSRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 IPQLAGRR48 pKa = 11.84 FAAKK52 pKa = 9.53 EE53 pKa = 3.92 AVMKK57 pKa = 10.93 ALGTGARR64 pKa = 11.84 GVSWRR69 pKa = 11.84 DD70 pKa = 2.94 IEE72 pKa = 4.4 VTRR75 pKa = 11.84 KK76 pKa = 9.99 RR77 pKa = 11.84 GFPPEE82 pKa = 3.89 IQLHH86 pKa = 5.13 GRR88 pKa = 11.84 ALARR92 pKa = 11.84 SRR94 pKa = 11.84 ILNLEE99 pKa = 4.57 RR100 pKa = 11.84 IAISISHH107 pKa = 5.79 SRR109 pKa = 11.84 HH110 pKa = 4.72 YY111 pKa = 10.46 AIAMAVGEE119 pKa = 4.48 SNEE122 pKa = 3.96 NCC124 pKa = 3.9
Molecular weight: 14.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.399
IPC_protein 10.423
Toseland 10.54
ProMoST 10.365
Dawson 10.643
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 10.643
Grimsley 10.701
Solomon 10.818
Lehninger 10.774
Nozaki 10.54
DTASelect 10.409
Thurlkill 10.555
EMBOSS 10.965
Sillero 10.584
Patrickios 10.423
IPC_peptide 10.818
IPC2_peptide 9.531
IPC2.peptide.svr19 8.684
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
589
0
589
180042
36
1474
305.7
33.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.81 ± 0.093
1.007 ± 0.036
4.951 ± 0.076
6.478 ± 0.094
4.033 ± 0.074
7.875 ± 0.086
2.142 ± 0.038
7.304 ± 0.087
4.116 ± 0.071
10.641 ± 0.119
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.434 ± 0.039
3.852 ± 0.048
4.636 ± 0.059
3.828 ± 0.051
5.414 ± 0.079
6.724 ± 0.072
5.654 ± 0.068
7.104 ± 0.081
1.208 ± 0.04
2.788 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here