Sulfolobus turreted icosahedral virus 2
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5IEY3|D5IEY3_9VIRU Uncharacterized protein OS=Sulfolobus turreted icosahedral virus 2 OX=754004 GN=STIV2_B67 PE=4 SV=1
MM1 pKa = 8.24 DD2 pKa = 4.09 EE3 pKa = 4.11 FKK5 pKa = 10.7 EE6 pKa = 4.21 VKK8 pKa = 10.03 EE9 pKa = 3.75 KK10 pKa = 10.77 FEE12 pKa = 4.16 RR13 pKa = 11.84 YY14 pKa = 9.61 KK15 pKa = 10.95 FDD17 pKa = 2.85 VEE19 pKa = 4.48 YY20 pKa = 10.29 IDD22 pKa = 5.41 RR23 pKa = 11.84 EE24 pKa = 4.15 YY25 pKa = 10.61 TINANYY31 pKa = 8.84 PKK33 pKa = 10.68 AEE35 pKa = 3.93 IPIGEE40 pKa = 4.16 RR41 pKa = 11.84 NGFTGIVVNNGTAILTITLVKK62 pKa = 9.99 VAHH65 pKa = 6.84 ASTTADD71 pKa = 3.61 LGVSTSDD78 pKa = 2.85 VGTIILAPGQVLQLTNFPLNEE99 pKa = 3.64 IKK101 pKa = 10.53 AVYY104 pKa = 9.6 NPNSPSSASSVVQVKK119 pKa = 10.01 GVIHH123 pKa = 6.75 PLFEE127 pKa = 4.47 VPVKK131 pKa = 11.04 LNVTDD136 pKa = 3.68 IVSIQQSNEE145 pKa = 3.48 NLLITTNTTINGGNYY160 pKa = 9.58 NDD162 pKa = 5.12 IVIQPNVTVNFDD174 pKa = 3.71 GFVTAKK180 pKa = 10.12 KK181 pKa = 10.27 IIMQSGSTVNVNGVLIVGEE200 pKa = 4.34 IEE202 pKa = 4.11 ADD204 pKa = 3.68 SPATLASSTPPINLTVNGVLVANYY228 pKa = 8.72 VQSDD232 pKa = 4.18 VNVEE236 pKa = 4.09 VASGFMGVKK245 pKa = 8.39 TLXYY249 pKa = 9.48 NGQVLYY255 pKa = 11.01 VNQGAFLFADD265 pKa = 3.5 TMIFSNNLNSLTYY278 pKa = 10.25 NSSISSAPGNIFANEE293 pKa = 4.01 IYY295 pKa = 10.68 FEE297 pKa = 4.34 TLLSQQLSVNMNITAKK313 pKa = 9.86 KK314 pKa = 9.57 IRR316 pKa = 11.84 MGGLMGGLMEE326 pKa = 5.41 IMAGLLTSLSISNLQFAINGNNLIIKK352 pKa = 7.33 MKK354 pKa = 9.93 KK355 pKa = 10.22 AIIFGFNANVNNSTIEE371 pKa = 3.95 IEE373 pKa = 4.21 DD374 pKa = 4.1 SIIASPSFYY383 pKa = 10.33 AYY385 pKa = 10.19 SGSSTIYY392 pKa = 10.34 IIIKK396 pKa = 10.38 GNTQIYY402 pKa = 8.94 QLSINSSLTSYY413 pKa = 10.78 IFFRR417 pKa = 11.84 QNEE420 pKa = 4.1 NGQGNIYY427 pKa = 10.16 LYY429 pKa = 9.98 NASFATSTYY438 pKa = 8.1 WRR440 pKa = 11.84 IDD442 pKa = 3.3 FGVTVTAYY450 pKa = 7.91 PASFSTSSSNSITVNGQLYY469 pKa = 9.2 MSSNLTTTSASSTTSSSVTIGLPFTVTGFGIAIISQNTNASANQGGYY516 pKa = 10.1 LAGNTIQMNNLQLSKK531 pKa = 10.91 GSYY534 pKa = 8.67 STTTQTGSGNNTYY547 pKa = 9.94 WYY549 pKa = 7.69 ITSIQGSAPGLYY561 pKa = 10.17 YY562 pKa = 10.77 LGVYY566 pKa = 10.43 DD567 pKa = 4.24 EE568 pKa = 5.04 TSGATNYY575 pKa = 9.6 IAEE578 pKa = 4.18 IVVYY582 pKa = 10.47 VGTVNQYY589 pKa = 11.38 ALSNPLGFVAEE600 pKa = 4.55 GGVADD605 pKa = 4.17 TSGNTYY611 pKa = 10.83 AILNSSASAGTITLSGLAAVV631 pKa = 4.65
Molecular weight: 67.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.579
IPC2_protein 4.698
IPC_protein 4.609
Toseland 4.469
ProMoST 4.711
Dawson 4.546
Bjellqvist 4.698
Wikipedia 4.406
Rodwell 4.457
Grimsley 4.38
Solomon 4.546
Lehninger 4.507
Nozaki 4.66
DTASelect 4.787
Thurlkill 4.457
EMBOSS 4.418
Sillero 4.724
Patrickios 0.668
IPC_peptide 4.558
IPC2_peptide 4.711
IPC2.peptide.svr19 4.673
Protein with the highest isoelectric point:
>tr|D5IEX4|D5IEX4_9VIRU Uncharacterized protein OS=Sulfolobus turreted icosahedral virus 2 OX=754004 GN=STIV2_C54a PE=4 SV=1
MM1 pKa = 7.26 AKK3 pKa = 9.94 KK4 pKa = 9.68 WIQKK8 pKa = 9.65 AIKK11 pKa = 9.9 HH12 pKa = 5.58 KK13 pKa = 10.63 GALKK17 pKa = 9.41 EE18 pKa = 4.22 WIKK21 pKa = 10.93 KK22 pKa = 7.08 EE23 pKa = 3.99 HH24 pKa = 6.14 PTLLRR29 pKa = 11.84 KK30 pKa = 10.35 DD31 pKa = 3.74 GEE33 pKa = 3.98 ISITKK38 pKa = 10.31 LEE40 pKa = 4.02 EE41 pKa = 4.08 FYY43 pKa = 10.87 RR44 pKa = 11.84 KK45 pKa = 9.78 HH46 pKa = 7.0 KK47 pKa = 10.95 DD48 pKa = 2.89 EE49 pKa = 4.5 LTPHH53 pKa = 7.49 RR54 pKa = 11.84 KK55 pKa = 8.92 RR56 pKa = 11.84 QILLALRR63 pKa = 11.84 LYY65 pKa = 10.86 RR66 pKa = 11.84 MRR68 pKa = 11.84 RR69 pKa = 11.84 KK70 pKa = 10.13 KK71 pKa = 10.55 SS72 pKa = 3.12
Molecular weight: 8.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.218
IPC2_protein 9.809
IPC_protein 10.072
Toseland 10.95
ProMoST 10.467
Dawson 11.008
Bjellqvist 10.613
Wikipedia 11.14
Rodwell 11.55
Grimsley 11.038
Solomon 11.082
Lehninger 11.067
Nozaki 10.921
DTASelect 10.613
Thurlkill 10.921
EMBOSS 11.33
Sillero 10.935
Patrickios 11.272
IPC_peptide 11.096
IPC2_peptide 9.033
IPC2.peptide.svr19 8.742
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
34
0
34
5395
54
631
158.7
17.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.228 ± 0.556
0.686 ± 0.177
4.133 ± 0.326
7.804 ± 0.905
4.133 ± 0.438
5.876 ± 0.559
1.538 ± 0.214
9.027 ± 0.544
8.378 ± 1.194
10.361 ± 0.467
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.909 ± 0.176
5.042 ± 0.637
3.911 ± 0.543
3.207 ± 0.298
4.059 ± 0.441
7.451 ± 0.779
5.338 ± 0.608
5.82 ± 0.445
0.704 ± 0.168
4.374 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here