Tapara virus
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S5SHW6|A0A1S5SHW6_9VIRU Nonstructural protein OS=Tapara virus OX=1926501 PE=4 SV=1
MM1 pKa = 7.82 SSYY4 pKa = 10.97 LYY6 pKa = 10.12 DD7 pKa = 3.65 RR8 pKa = 11.84 PIIFRR13 pKa = 11.84 QSQEE17 pKa = 4.12 DD18 pKa = 4.13 CSVQYY23 pKa = 11.02 LAFNSFTDD31 pKa = 4.7 RR32 pKa = 11.84 EE33 pKa = 4.51 VCVNNDD39 pKa = 2.96 MEE41 pKa = 4.93 IPVEE45 pKa = 4.32 NYY47 pKa = 7.4 MASCAFRR54 pKa = 11.84 EE55 pKa = 4.26 SLQDD59 pKa = 3.71 FYY61 pKa = 11.6 RR62 pKa = 11.84 AGTLPLKK69 pKa = 10.08 WGDD72 pKa = 3.57 EE73 pKa = 4.24 MPVNRR78 pKa = 11.84 VTKK81 pKa = 10.32 GSYY84 pKa = 10.25 RR85 pKa = 11.84 LMDD88 pKa = 3.66 GVIEE92 pKa = 4.17 SLSRR96 pKa = 11.84 FSRR99 pKa = 11.84 EE100 pKa = 4.31 DD101 pKa = 3.05 IIKK104 pKa = 10.35 SYY106 pKa = 10.73 LPNVQNALSWPLGYY120 pKa = 7.68 PTLDD124 pKa = 3.91 FIKK127 pKa = 10.19 ACVMDD132 pKa = 3.87 TPLYY136 pKa = 8.61 TRR138 pKa = 11.84 KK139 pKa = 8.48 STHH142 pKa = 5.38 ATLIFRR148 pKa = 11.84 TGQPADD154 pKa = 4.17 CLDD157 pKa = 3.4 QSFVNSHH164 pKa = 5.49 RR165 pKa = 11.84 RR166 pKa = 11.84 IVMEE170 pKa = 3.88 SVTRR174 pKa = 11.84 GFDD177 pKa = 3.09 VKK179 pKa = 10.3 TFTGQNLVRR188 pKa = 11.84 DD189 pKa = 4.14 IASLQCVRR197 pKa = 11.84 VLNAYY202 pKa = 9.45 QADD205 pKa = 4.03 LLYY208 pKa = 10.93 CGVSTEE214 pKa = 4.18 LTKK217 pKa = 10.67 EE218 pKa = 3.9 LSKK221 pKa = 11.06 LRR223 pKa = 11.84 IIHH226 pKa = 7.12 DD227 pKa = 3.64 GHH229 pKa = 6.42 PQNPLGNQRR238 pKa = 11.84 WIASPDD244 pKa = 3.02 SWDD247 pKa = 3.45 YY248 pKa = 11.41 RR249 pKa = 11.84 PNPDD253 pKa = 3.78 VEE255 pKa = 5.23 LDD257 pKa = 3.46 FDD259 pKa = 5.65 LDD261 pKa = 3.62 ISDD264 pKa = 4.13 SSEE267 pKa = 4.12 DD268 pKa = 3.59 EE269 pKa = 4.07
Molecular weight: 30.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.702
IPC2_protein 4.787
IPC_protein 4.736
Toseland 4.571
ProMoST 4.813
Dawson 4.711
Bjellqvist 4.876
Wikipedia 4.609
Rodwell 4.584
Grimsley 4.482
Solomon 4.711
Lehninger 4.66
Nozaki 4.813
DTASelect 5.041
Thurlkill 4.596
EMBOSS 4.635
Sillero 4.863
Patrickios 3.681
IPC_peptide 4.711
IPC2_peptide 4.851
IPC2.peptide.svr19 4.834
Protein with the highest isoelectric point:
>tr|A0A1S5SHW6|A0A1S5SHW6_9VIRU Nonstructural protein OS=Tapara virus OX=1926501 PE=4 SV=1
MM1 pKa = 7.85 SDD3 pKa = 3.66 YY4 pKa = 10.91 EE5 pKa = 4.1 KK6 pKa = 11.01 LAIEE10 pKa = 4.28 LQGEE14 pKa = 4.54 PIDD17 pKa = 4.04 KK18 pKa = 10.55 NAIQRR23 pKa = 11.84 WMSEE27 pKa = 3.61 FAYY30 pKa = 10.28 EE31 pKa = 4.76 GFDD34 pKa = 3.17 AANIVKK40 pKa = 10.08 LVHH43 pKa = 6.01 EE44 pKa = 4.37 RR45 pKa = 11.84 AKK47 pKa = 10.89 AAGRR51 pKa = 11.84 DD52 pKa = 3.13 WKK54 pKa = 11.16 LDD56 pKa = 3.6 VKK58 pKa = 11.23 KK59 pKa = 10.36 MIVLALTRR67 pKa = 11.84 GNKK70 pKa = 7.3 VDD72 pKa = 4.16 KK73 pKa = 9.79 MIKK76 pKa = 10.1 KK77 pKa = 9.36 MSEE80 pKa = 3.39 KK81 pKa = 10.4 GAATVTEE88 pKa = 4.22 LVRR91 pKa = 11.84 VYY93 pKa = 10.58 SLKK96 pKa = 10.82 SGNPTRR102 pKa = 11.84 DD103 pKa = 3.59 DD104 pKa = 3.55 LTLSRR109 pKa = 11.84 VAAAFAGYY117 pKa = 7.77 TCQALPHH124 pKa = 6.35 LAEE127 pKa = 5.64 AIPVSGSQMDD137 pKa = 4.08 EE138 pKa = 3.94 VVKK141 pKa = 10.78 NYY143 pKa = 7.42 PRR145 pKa = 11.84 PMMHH149 pKa = 7.17 PAFSGLIDD157 pKa = 3.48 PTFKK161 pKa = 11.22 EE162 pKa = 4.13 EE163 pKa = 4.39 TKK165 pKa = 10.67 DD166 pKa = 3.5 ALINAHH172 pKa = 5.93 SLFMYY177 pKa = 10.33 HH178 pKa = 5.83 FTVQINEE185 pKa = 3.93 KK186 pKa = 9.66 MRR188 pKa = 11.84 GKK190 pKa = 9.96 SDD192 pKa = 3.38 KK193 pKa = 10.7 EE194 pKa = 4.14 IISSFQQPMYY204 pKa = 10.88 AAINSGFMKK213 pKa = 10.74 GEE215 pKa = 3.8 TRR217 pKa = 11.84 RR218 pKa = 11.84 AMLRR222 pKa = 11.84 TLGIVDD228 pKa = 4.38 ANWVPSSIVQEE239 pKa = 3.63 AAKK242 pKa = 10.01 IYY244 pKa = 9.91 KK245 pKa = 9.78 EE246 pKa = 4.31 KK247 pKa = 11.41 YY248 pKa = 9.27 NDD250 pKa = 3.2
Molecular weight: 28.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.32
IPC2_protein 8.273
IPC_protein 8.229
Toseland 8.96
ProMoST 8.697
Dawson 9.224
Bjellqvist 8.931
Wikipedia 9.37
Rodwell 9.458
Grimsley 9.268
Solomon 9.326
Lehninger 9.297
Nozaki 9.019
DTASelect 8.902
Thurlkill 9.063
EMBOSS 9.37
Sillero 9.18
Patrickios 4.838
IPC_peptide 9.311
IPC2_peptide 7.483
IPC2.peptide.svr19 7.585
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3951
250
2089
987.8
112.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.961 ± 0.835
2.455 ± 0.826
5.695 ± 0.637
7.669 ± 0.57
4.885 ± 0.365
5.669 ± 0.335
2.556 ± 0.247
6.96 ± 0.388
7.213 ± 0.731
8.226 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.265 ± 0.361
3.872 ± 0.496
3.569 ± 0.394
2.784 ± 0.374
5.442 ± 0.313
9.415 ± 0.446
5.29 ± 0.474
5.897 ± 0.194
1.215 ± 0.06
2.961 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here