Rhynchosia yellow mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8TES4|C8TES4_9GEMI Movement protein BC1 OS=Rhynchosia yellow mosaic virus OX=529680 GN=bc1 PE=3 SV=2
MM1 pKa = 8.04DD2 pKa = 5.02NYY4 pKa = 11.14SGAVVNNKK12 pKa = 9.82YY13 pKa = 10.02IEE15 pKa = 4.33SKK17 pKa = 9.13RR18 pKa = 11.84CEE20 pKa = 3.84YY21 pKa = 10.88RR22 pKa = 11.84LTNNEE27 pKa = 4.22TPIMLQFPSSLEE39 pKa = 3.87QTKK42 pKa = 10.0VRR44 pKa = 11.84MLGKK48 pKa = 10.07CMKK51 pKa = 9.46VDD53 pKa = 4.32HH54 pKa = 6.68IVIEE58 pKa = 4.22YY59 pKa = 10.2RR60 pKa = 11.84NQVPFNATGSVIVTIRR76 pKa = 11.84DD77 pKa = 3.21TRR79 pKa = 11.84LSHH82 pKa = 5.99EE83 pKa = 4.3QAAQAAFTFPIACNVDD99 pKa = 2.84LHH101 pKa = 6.36YY102 pKa = 10.7FSSSFFSLKK111 pKa = 10.84DD112 pKa = 3.25EE113 pKa = 4.68TPWEE117 pKa = 3.87LVYY120 pKa = 10.68KK121 pKa = 10.83VEE123 pKa = 4.75DD124 pKa = 3.71SNVIDD129 pKa = 3.77GTTFAQIKK137 pKa = 10.06GKK139 pKa = 10.65LKK141 pKa = 10.61LSSAKK146 pKa = 10.11HH147 pKa = 4.86STDD150 pKa = 2.97IRR152 pKa = 11.84FKK154 pKa = 10.8PPTINILSKK163 pKa = 11.0DD164 pKa = 3.7FTKK167 pKa = 10.83DD168 pKa = 3.55CVDD171 pKa = 4.22FWSVDD176 pKa = 2.94KK177 pKa = 10.67PKK179 pKa = 10.5PIRR182 pKa = 11.84RR183 pKa = 11.84LLNPGPGYY191 pKa = 10.89GPDD194 pKa = 2.72GHH196 pKa = 6.38EE197 pKa = 3.62RR198 pKa = 11.84HH199 pKa = 6.03KK200 pKa = 10.82PIMLQPGEE208 pKa = 4.1TWATRR213 pKa = 11.84STIGRR218 pKa = 11.84TTSMRR223 pKa = 11.84YY224 pKa = 9.88APTEE228 pKa = 4.28RR229 pKa = 11.84IALDD233 pKa = 4.06DD234 pKa = 4.46KK235 pKa = 10.8PSCSEE240 pKa = 3.54AEE242 pKa = 4.11YY243 pKa = 10.08PLKK246 pKa = 10.78HH247 pKa = 5.04MHH249 pKa = 6.96KK250 pKa = 10.15LPEE253 pKa = 4.5SSLDD257 pKa = 3.61PGDD260 pKa = 4.45SVSQTTSNAMTKK272 pKa = 10.02QDD274 pKa = 3.42IEE276 pKa = 4.25EE277 pKa = 4.99LIEE280 pKa = 4.04TTINKK285 pKa = 9.49CLITQRR291 pKa = 11.84STISKK296 pKa = 9.61PLL298 pKa = 3.49

Molecular weight: 33.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7TPF7|C7TPF7_9GEMI Replication enhancer OS=Rhynchosia yellow mosaic virus OX=529680 GN=ac3 PE=3 SV=1
MM1 pKa = 7.77VLILGCFLMVIHH13 pKa = 5.91YY14 pKa = 9.11VVVYY18 pKa = 7.8TIKK21 pKa = 10.54PINYY25 pKa = 8.58SLFLARR31 pKa = 11.84ILTTGYY37 pKa = 11.78SMMKK41 pKa = 10.17LAQNLITITQIVLHH55 pKa = 6.54RR56 pKa = 11.84SGTRR60 pKa = 11.84LIIIHH65 pKa = 5.99VKK67 pKa = 10.02NLSKK71 pKa = 10.16IHH73 pKa = 6.31RR74 pKa = 11.84RR75 pKa = 11.84SKK77 pKa = 10.41RR78 pKa = 11.84PPVSNEE84 pKa = 3.87LKK86 pKa = 9.08HH87 pKa = 5.66HH88 pKa = 5.16TVGVILGLDD97 pKa = 3.59VFIHH101 pKa = 6.67PNFPCNVNRR110 pKa = 11.84LDD112 pKa = 3.96AKK114 pKa = 10.07TFADD118 pKa = 3.69TMSNTVTTCHH128 pKa = 6.23IRR130 pKa = 11.84NAYY133 pKa = 9.83HH134 pKa = 6.26LTNMM138 pKa = 4.64

Molecular weight: 15.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1803

97

364

200.3

22.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.713 ± 0.583

2.219 ± 0.306

4.603 ± 0.454

4.215 ± 0.637

4.382 ± 0.373

5.214 ± 0.572

3.938 ± 0.434

5.214 ± 0.754

6.434 ± 0.327

8.319 ± 0.858

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.662 ± 0.419

5.935 ± 0.556

5.99 ± 0.592

4.104 ± 0.457

6.6 ± 0.821

7.377 ± 0.621

6.045 ± 0.831

6.101 ± 0.677

0.998 ± 0.154

3.938 ± 0.418
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski