Gaiella sp. SCGC AG-212-M14

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Rubrobacteria; Gaiellales; Gaiellaceae; Gaiella; unclassified Gaiella

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 915 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177R6C3|A0A177R6C3_9ACTN Glyco_trans_2-like domain-containing protein OS=Gaiella sp. SCGC AG-212-M14 OX=1799656 GN=AYO48_03790 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 3.64GRR4 pKa = 11.84AAAFQSVDD12 pKa = 3.02IEE14 pKa = 4.18IDD16 pKa = 2.8AGRR19 pKa = 11.84PLGIRR24 pKa = 11.84VAWAGGGAHH33 pKa = 6.92FLAIIGYY40 pKa = 9.68LEE42 pKa = 4.72DD43 pKa = 3.66VEE45 pKa = 5.65NYY47 pKa = 9.79IAVDD51 pKa = 3.42DD52 pKa = 4.96PIYY55 pKa = 10.85GKK57 pKa = 10.62SDD59 pKa = 3.24LTYY62 pKa = 8.73DD63 pKa = 3.9TLRR66 pKa = 11.84SSYY69 pKa = 10.16QGSGTWTHH77 pKa = 6.17TYY79 pKa = 8.19YY80 pKa = 10.21TQAA83 pKa = 3.88

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177R532|A0A177R532_9ACTN Uncharacterized protein OS=Gaiella sp. SCGC AG-212-M14 OX=1799656 GN=AYO48_00780 PE=4 SV=1
MM1 pKa = 7.47SPASTEE7 pKa = 3.8QRR9 pKa = 11.84SRR11 pKa = 11.84ASIRR15 pKa = 11.84RR16 pKa = 11.84LIAPLALAQFICSFAGSNMNVMISDD41 pKa = 3.71ISKK44 pKa = 10.76DD45 pKa = 3.3LNTTVKK51 pKa = 10.42GVQTTITLFLLIMAILMIPCSKK73 pKa = 9.91LTDD76 pKa = 2.64RR77 pKa = 11.84WGRR80 pKa = 11.84KK81 pKa = 8.17RR82 pKa = 11.84CFTMGLTLYY91 pKa = 10.93GIGALVSAAAPNLGVLILGNSVFEE115 pKa = 4.51GVGTALLIPPVYY127 pKa = 10.28ILATLWFADD136 pKa = 3.54VTSRR140 pKa = 11.84AWAFGVISGLGGVGAAAGPLIGGVITTGISWRR172 pKa = 11.84AAFVFQALVIATILLLSRR190 pKa = 11.84RR191 pKa = 11.84LVDD194 pKa = 4.82PLPADD199 pKa = 3.55PARR202 pKa = 11.84PFDD205 pKa = 3.84AVGAILSAVGMFFVVFGILQAGSNNVLFVICLGIGAAFLASFFLFIRR252 pKa = 11.84RR253 pKa = 11.84RR254 pKa = 11.84EE255 pKa = 4.08RR256 pKa = 11.84AGKK259 pKa = 10.03EE260 pKa = 3.67PLLSTRR266 pKa = 11.84LFRR269 pKa = 11.84NRR271 pKa = 11.84TCNLALVTQNVQWLMLLGTSFVVSVFLQTEE301 pKa = 4.41RR302 pKa = 11.84GYY304 pKa = 11.04SAIKK308 pKa = 9.21TGVIFTAATVGILISSFAAEE328 pKa = 3.67RR329 pKa = 11.84WARR332 pKa = 11.84KK333 pKa = 8.75YY334 pKa = 10.08AQKK337 pKa = 9.72TLIVTGFVVTIVGMGLLLGLVKK359 pKa = 10.71ASSSDD364 pKa = 3.02WAFVPGLFLIGFGVGAMLTPSVNVVQSSFPEE395 pKa = 3.99ALQGEE400 pKa = 4.57ISGLSRR406 pKa = 11.84SVSNLGSSFGTAIAGTIIVSVVAPGYY432 pKa = 10.2RR433 pKa = 11.84SYY435 pKa = 11.71AWALVSLVLIGLIGLGAALLLPSKK459 pKa = 10.65LEE461 pKa = 4.07RR462 pKa = 11.84PAA464 pKa = 5.03

Molecular weight: 48.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

915

0

915

275724

53

3217

301.3

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.604 ± 0.11

0.733 ± 0.021

5.461 ± 0.059

6.116 ± 0.126

3.493 ± 0.048

8.898 ± 0.074

2.034 ± 0.042

4.292 ± 0.059

2.489 ± 0.068

10.751 ± 0.128

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.591 ± 0.033

2.189 ± 0.061

5.307 ± 0.054

2.865 ± 0.046

7.798 ± 0.115

5.646 ± 0.118

5.507 ± 0.138

8.502 ± 0.076

1.534 ± 0.037

2.191 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski