Amino acid dipepetide frequency for Gaiella sp. SCGC AG-212-M14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.152AlaAla: 18.152 ± 0.387
1.052AlaCys: 1.052 ± 0.061
6.361AlaAsp: 6.361 ± 0.154
7.42AlaGlu: 7.42 ± 0.255
4.298AlaPhe: 4.298 ± 0.143
11.794AlaGly: 11.794 ± 0.262
2.318AlaHis: 2.318 ± 0.103
5.553AlaIle: 5.553 ± 0.16
3.304AlaLys: 3.304 ± 0.128
14.823AlaLeu: 14.823 ± 0.36
2.158AlaMet: 2.158 ± 0.092
2.408AlaAsn: 2.408 ± 0.116
5.418AlaPro: 5.418 ± 0.182
3.449AlaGln: 3.449 ± 0.127
9.578AlaArg: 9.578 ± 0.231
6.641AlaSer: 6.641 ± 0.28
6.637AlaThr: 6.637 ± 0.248
10.333AlaVal: 10.333 ± 0.232
1.792AlaTrp: 1.792 ± 0.093
2.55AlaTyr: 2.55 ± 0.109
0.0AlaXaa: 0.0 ± 0.0
Cys
0.823CysAla: 0.823 ± 0.051
0.12CysCys: 0.12 ± 0.024
0.377CysAsp: 0.377 ± 0.038
0.348CysGlu: 0.348 ± 0.036
0.258CysPhe: 0.258 ± 0.03
0.928CysGly: 0.928 ± 0.056
0.174CysHis: 0.174 ± 0.026
0.363CysIle: 0.363 ± 0.038
0.16CysLys: 0.16 ± 0.022
0.62CysLeu: 0.62 ± 0.052
0.08CysMet: 0.08 ± 0.015
0.145CysAsn: 0.145 ± 0.025
0.33CysPro: 0.33 ± 0.035
0.127CysGln: 0.127 ± 0.02
0.53CysArg: 0.53 ± 0.047
0.544CysSer: 0.544 ± 0.056
0.442CysThr: 0.442 ± 0.088
0.627CysVal: 0.627 ± 0.051
0.131CysTrp: 0.131 ± 0.021
0.203CysTyr: 0.203 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
6.641AspAla: 6.641 ± 0.18
0.301AspCys: 0.301 ± 0.034
3.478AspAsp: 3.478 ± 0.129
4.218AspGlu: 4.218 ± 0.159
1.839AspPhe: 1.839 ± 0.087
5.005AspGly: 5.005 ± 0.159
1.208AspHis: 1.208 ± 0.082
1.686AspIle: 1.686 ± 0.08
1.121AspLys: 1.121 ± 0.084
5.749AspLeu: 5.749 ± 0.158
0.631AspMet: 0.631 ± 0.055
1.048AspAsn: 1.048 ± 0.126
3.652AspPro: 3.652 ± 0.127
1.462AspGln: 1.462 ± 0.079
4.508AspArg: 4.508 ± 0.131
2.383AspSer: 2.383 ± 0.107
2.459AspThr: 2.459 ± 0.109
5.02AspVal: 5.02 ± 0.153
0.885AspTrp: 0.885 ± 0.057
1.32AspTyr: 1.32 ± 0.071
0.0AspXaa: 0.0 ± 0.0
Glu
7.105GluAla: 7.105 ± 0.207
0.345GluCys: 0.345 ± 0.039
2.687GluAsp: 2.687 ± 0.124
4.062GluGlu: 4.062 ± 0.169
1.748GluPhe: 1.748 ± 0.084
4.461GluGly: 4.461 ± 0.18
1.567GluHis: 1.567 ± 0.076
2.862GluIle: 2.862 ± 0.116
1.284GluLys: 1.284 ± 0.08
8.342GluLeu: 8.342 ± 0.25
0.852GluMet: 0.852 ± 0.061
1.099GluAsn: 1.099 ± 0.071
3.188GluPro: 3.188 ± 0.144
2.499GluGln: 2.499 ± 0.104
6.507GluArg: 6.507 ± 0.212
2.756GluSer: 2.756 ± 0.115
3.076GluThr: 3.076 ± 0.118
4.668GluVal: 4.668 ± 0.167
0.943GluTrp: 0.943 ± 0.065
1.113GluTyr: 1.113 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
4.777PheAla: 4.777 ± 0.159
0.276PheCys: 0.276 ± 0.03
2.27PheAsp: 2.27 ± 0.09
2.085PheGlu: 2.085 ± 0.106
1.4PhePhe: 1.4 ± 0.085
3.551PheGly: 3.551 ± 0.126
0.7PheHis: 0.7 ± 0.059
1.193PheIle: 1.193 ± 0.067
0.802PheLys: 0.802 ± 0.057
3.355PheLeu: 3.355 ± 0.126
0.519PheMet: 0.519 ± 0.045
0.791PheAsn: 0.791 ± 0.064
1.654PhePro: 1.654 ± 0.08
0.925PheGln: 0.925 ± 0.064
2.22PheArg: 2.22 ± 0.101
2.027PheSer: 2.027 ± 0.087
1.806PheThr: 1.806 ± 0.075
3.134PheVal: 3.134 ± 0.119
0.664PheTrp: 0.664 ± 0.058
0.787PheTyr: 0.787 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
10.122GlyAla: 10.122 ± 0.244
0.765GlyCys: 0.765 ± 0.055
4.795GlyAsp: 4.795 ± 0.15
5.074GlyGlu: 5.074 ± 0.148
3.42GlyPhe: 3.42 ± 0.111
7.91GlyGly: 7.91 ± 0.292
1.81GlyHis: 1.81 ± 0.086
4.305GlyIle: 4.305 ± 0.163
2.807GlyLys: 2.807 ± 0.134
9.024GlyLeu: 9.024 ± 0.218
1.795GlyMet: 1.795 ± 0.079
2.339GlyAsn: 2.339 ± 0.171
4.077GlyPro: 4.077 ± 0.13
2.415GlyGln: 2.415 ± 0.103
6.532GlyArg: 6.532 ± 0.21
5.923GlySer: 5.923 ± 0.301
5.103GlyThr: 5.103 ± 0.187
7.029GlyVal: 7.029 ± 0.188
1.599GlyTrp: 1.599 ± 0.081
2.129GlyTyr: 2.129 ± 0.092
0.0GlyXaa: 0.0 ± 0.0
His
2.455HisAla: 2.455 ± 0.091
0.116HisCys: 0.116 ± 0.017
1.298HisAsp: 1.298 ± 0.068
1.28HisGlu: 1.28 ± 0.079
0.606HisPhe: 0.606 ± 0.044
2.053HisGly: 2.053 ± 0.085
0.508HisHis: 0.508 ± 0.045
0.736HisIle: 0.736 ± 0.048
0.355HisLys: 0.355 ± 0.038
2.154HisLeu: 2.154 ± 0.091
0.355HisMet: 0.355 ± 0.039
0.395HisAsn: 0.395 ± 0.042
1.375HisPro: 1.375 ± 0.073
0.522HisGln: 0.522 ± 0.044
1.472HisArg: 1.472 ± 0.073
0.986HisSer: 0.986 ± 0.068
1.005HisThr: 1.005 ± 0.076
1.774HisVal: 1.774 ± 0.078
0.352HisTrp: 0.352 ± 0.037
0.537HisTyr: 0.537 ± 0.043
0.0HisXaa: 0.0 ± 0.0
Ile
6.332IleAla: 6.332 ± 0.169
0.363IleCys: 0.363 ± 0.038
2.746IleAsp: 2.746 ± 0.102
3.018IleGlu: 3.018 ± 0.137
1.48IlePhe: 1.48 ± 0.083
4.512IleGly: 4.512 ± 0.142
0.885IleHis: 0.885 ± 0.055
1.335IleIle: 1.335 ± 0.085
0.91IleLys: 0.91 ± 0.07
3.489IleLeu: 3.489 ± 0.116
0.428IleMet: 0.428 ± 0.038
0.82IleAsn: 0.82 ± 0.051
2.285IlePro: 2.285 ± 0.099
1.016IleGln: 1.016 ± 0.063
2.88IleArg: 2.88 ± 0.113
2.368IleSer: 2.368 ± 0.093
2.104IleThr: 2.104 ± 0.103
4.523IleVal: 4.523 ± 0.159
0.584IleTrp: 0.584 ± 0.046
0.849IleTyr: 0.849 ± 0.062
0.0IleXaa: 0.0 ± 0.0
Lys
2.731LysAla: 2.731 ± 0.134
0.087LysCys: 0.087 ± 0.018
1.117LysAsp: 1.117 ± 0.084
1.335LysGlu: 1.335 ± 0.085
0.631LysPhe: 0.631 ± 0.06
1.926LysGly: 1.926 ± 0.106
0.493LysHis: 0.493 ± 0.046
1.128LysIle: 1.128 ± 0.078
0.939LysLys: 0.939 ± 0.106
2.804LysLeu: 2.804 ± 0.106
0.439LysMet: 0.439 ± 0.049
0.602LysAsn: 0.602 ± 0.057
1.647LysPro: 1.647 ± 0.1
0.972LysGln: 0.972 ± 0.07
2.046LysArg: 2.046 ± 0.082
1.407LysSer: 1.407 ± 0.081
1.614LysThr: 1.614 ± 0.088
2.114LysVal: 2.114 ± 0.103
0.334LysTrp: 0.334 ± 0.039
0.522LysTyr: 0.522 ± 0.051
0.0LysXaa: 0.0 ± 0.0
Leu
15.36LeuAla: 15.36 ± 0.325
0.834LeuCys: 0.834 ± 0.064
6.108LeuAsp: 6.108 ± 0.166
6.905LeuGlu: 6.905 ± 0.226
3.409LeuPhe: 3.409 ± 0.143
9.872LeuGly: 9.872 ± 0.221
2.111LeuHis: 2.111 ± 0.082
4.359LeuIle: 4.359 ± 0.161
2.318LeuLys: 2.318 ± 0.119
11.091LeuLeu: 11.091 ± 0.301
1.458LeuMet: 1.458 ± 0.074
2.064LeuAsn: 2.064 ± 0.083
5.389LeuPro: 5.389 ± 0.145
2.76LeuGln: 2.76 ± 0.102
8.262LeuArg: 8.262 ± 0.215
5.999LeuSer: 5.999 ± 0.162
5.894LeuThr: 5.894 ± 0.186
9.912LeuVal: 9.912 ± 0.242
1.342LeuTrp: 1.342 ± 0.079
2.064LeuTyr: 2.064 ± 0.096
0.0LeuXaa: 0.0 ± 0.0
Met
1.861MetAla: 1.861 ± 0.08
0.094MetCys: 0.094 ± 0.017
0.656MetAsp: 0.656 ± 0.054
0.718MetGlu: 0.718 ± 0.05
0.439MetPhe: 0.439 ± 0.041
1.092MetGly: 1.092 ± 0.053
0.276MetHis: 0.276 ± 0.031
0.816MetIle: 0.816 ± 0.054
0.468MetLys: 0.468 ± 0.044
1.792MetLeu: 1.792 ± 0.086
0.265MetMet: 0.265 ± 0.03
0.515MetAsn: 0.515 ± 0.042
1.012MetPro: 1.012 ± 0.058
0.551MetGln: 0.551 ± 0.039
1.487MetArg: 1.487 ± 0.074
1.132MetSer: 1.132 ± 0.062
1.219MetThr: 1.219 ± 0.072
1.099MetVal: 1.099 ± 0.071
0.199MetTrp: 0.199 ± 0.028
0.225MetTyr: 0.225 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
2.64AsnAla: 2.64 ± 0.141
0.149AsnCys: 0.149 ± 0.025
1.168AsnAsp: 1.168 ± 0.081
1.19AsnGlu: 1.19 ± 0.069
0.794AsnPhe: 0.794 ± 0.062
2.263AsnGly: 2.263 ± 0.117
0.497AsnHis: 0.497 ± 0.042
0.892AsnIle: 0.892 ± 0.063
0.439AsnLys: 0.439 ± 0.05
1.991AsnLeu: 1.991 ± 0.081
0.33AsnMet: 0.33 ± 0.04
0.555AsnAsn: 0.555 ± 0.065
1.549AsnPro: 1.549 ± 0.078
0.526AsnGln: 0.526 ± 0.048
1.567AsnArg: 1.567 ± 0.077
1.244AsnSer: 1.244 ± 0.101
1.295AsnThr: 1.295 ± 0.158
1.98AsnVal: 1.98 ± 0.098
0.359AsnTrp: 0.359 ± 0.038
0.461AsnTyr: 0.461 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
6.245ProAla: 6.245 ± 0.194
0.272ProCys: 0.272 ± 0.036
3.373ProAsp: 3.373 ± 0.117
3.485ProGlu: 3.485 ± 0.137
1.962ProPhe: 1.962 ± 0.087
4.802ProGly: 4.802 ± 0.153
1.045ProHis: 1.045 ± 0.06
2.089ProIle: 2.089 ± 0.082
1.534ProLys: 1.534 ± 0.089
5.35ProLeu: 5.35 ± 0.139
0.841ProMet: 0.841 ± 0.056
1.298ProAsn: 1.298 ± 0.083
3.018ProPro: 3.018 ± 0.116
1.596ProGln: 1.596 ± 0.082
3.471ProArg: 3.471 ± 0.131
3.097ProSer: 3.097 ± 0.13
2.996ProThr: 2.996 ± 0.151
4.472ProVal: 4.472 ± 0.137
0.918ProTrp: 0.918 ± 0.056
1.208ProTyr: 1.208 ± 0.058
0.0ProXaa: 0.0 ± 0.0
Gln
3.536GlnAla: 3.536 ± 0.131
0.131GlnCys: 0.131 ± 0.021
1.066GlnAsp: 1.066 ± 0.057
1.534GlnGlu: 1.534 ± 0.083
0.885GlnPhe: 0.885 ± 0.057
2.06GlnGly: 2.06 ± 0.086
0.635GlnHis: 0.635 ± 0.043
1.552GlnIle: 1.552 ± 0.075
0.812GlnLys: 0.812 ± 0.059
3.3GlnLeu: 3.3 ± 0.101
0.504GlnMet: 0.504 ± 0.041
0.754GlnAsn: 0.754 ± 0.059
1.639GlnPro: 1.639 ± 0.076
1.066GlnGln: 1.066 ± 0.073
2.503GlnArg: 2.503 ± 0.103
1.552GlnSer: 1.552 ± 0.111
1.726GlnThr: 1.726 ± 0.084
2.372GlnVal: 2.372 ± 0.114
0.359GlnTrp: 0.359 ± 0.037
0.664GlnTyr: 0.664 ± 0.056
0.0GlnXaa: 0.0 ± 0.0
Arg
9.256ArgAla: 9.256 ± 0.256
0.555ArgCys: 0.555 ± 0.046
4.374ArgAsp: 4.374 ± 0.147
5.288ArgGlu: 5.288 ± 0.198
2.872ArgPhe: 2.872 ± 0.113
5.846ArgGly: 5.846 ± 0.156
1.625ArgHis: 1.625 ± 0.088
3.721ArgIle: 3.721 ± 0.115
1.937ArgLys: 1.937 ± 0.109
8.632ArgLeu: 8.632 ± 0.25
1.378ArgMet: 1.378 ± 0.073
1.509ArgAsn: 1.509 ± 0.078
3.754ArgPro: 3.754 ± 0.145
2.194ArgGln: 2.194 ± 0.094
7.377ArgArg: 7.377 ± 0.219
4.432ArgSer: 4.432 ± 0.148
3.935ArgThr: 3.935 ± 0.126
6.166ArgVal: 6.166 ± 0.154
1.251ArgTrp: 1.251 ± 0.072
1.879ArgTyr: 1.879 ± 0.082
0.0ArgXaa: 0.0 ± 0.0
Ser
6.401SerAla: 6.401 ± 0.247
0.475SerCys: 0.475 ± 0.09
2.97SerAsp: 2.97 ± 0.17
3.007SerGlu: 3.007 ± 0.107
2.27SerPhe: 2.27 ± 0.102
5.886SerGly: 5.886 ± 0.232
1.066SerHis: 1.066 ± 0.064
2.307SerIle: 2.307 ± 0.114
1.447SerLys: 1.447 ± 0.102
5.821SerLeu: 5.821 ± 0.172
1.045SerMet: 1.045 ± 0.065
1.425SerAsn: 1.425 ± 0.097
3.34SerPro: 3.34 ± 0.144
1.636SerGln: 1.636 ± 0.102
3.964SerArg: 3.964 ± 0.112
3.772SerSer: 3.772 ± 0.189
3.199SerThr: 3.199 ± 0.17
4.189SerVal: 4.189 ± 0.158
0.932SerTrp: 0.932 ± 0.079
1.302SerTyr: 1.302 ± 0.077
0.0SerXaa: 0.0 ± 0.0
Thr
6.568ThrAla: 6.568 ± 0.285
0.403ThrCys: 0.403 ± 0.046
2.691ThrAsp: 2.691 ± 0.178
2.767ThrGlu: 2.767 ± 0.114
2.093ThrPhe: 2.093 ± 0.098
4.929ThrGly: 4.929 ± 0.196
1.088ThrHis: 1.088 ± 0.066
2.898ThrIle: 2.898 ± 0.136
1.429ThrLys: 1.429 ± 0.083
5.883ThrLeu: 5.883 ± 0.157
1.012ThrMet: 1.012 ± 0.054
1.331ThrAsn: 1.331 ± 0.094
3.358ThrPro: 3.358 ± 0.146
1.48ThrGln: 1.48 ± 0.108
3.634ThrArg: 3.634 ± 0.121
3.387ThrSer: 3.387 ± 0.226
3.398ThrThr: 3.398 ± 0.233
4.454ThrVal: 4.454 ± 0.179
0.874ThrTrp: 0.874 ± 0.069
1.389ThrTyr: 1.389 ± 0.081
0.0ThrXaa: 0.0 ± 0.0
Val
11.026ValAla: 11.026 ± 0.264
0.707ValCys: 0.707 ± 0.06
4.896ValAsp: 4.896 ± 0.14
5.357ValGlu: 5.357 ± 0.203
3.021ValPhe: 3.021 ± 0.12
7.047ValGly: 7.047 ± 0.172
1.639ValHis: 1.639 ± 0.075
3.612ValIle: 3.612 ± 0.115
1.886ValLys: 1.886 ± 0.096
9.154ValLeu: 9.154 ± 0.226
1.302ValMet: 1.302 ± 0.071
1.879ValAsn: 1.879 ± 0.095
4.338ValPro: 4.338 ± 0.15
2.325ValGln: 2.325 ± 0.099
6.151ValArg: 6.151 ± 0.149
4.617ValSer: 4.617 ± 0.131
4.983ValThr: 4.983 ± 0.184
8.153ValVal: 8.153 ± 0.212
1.157ValTrp: 1.157 ± 0.066
1.77ValTyr: 1.77 ± 0.087
0.0ValXaa: 0.0 ± 0.0
Trp
1.618TrpAla: 1.618 ± 0.068
0.109TrpCys: 0.109 ± 0.024
0.773TrpAsp: 0.773 ± 0.057
0.754TrpGlu: 0.754 ± 0.058
0.624TrpPhe: 0.624 ± 0.047
0.943TrpGly: 0.943 ± 0.06
0.294TrpHis: 0.294 ± 0.033
0.827TrpIle: 0.827 ± 0.056
0.482TrpLys: 0.482 ± 0.05
1.81TrpLeu: 1.81 ± 0.09
0.218TrpMet: 0.218 ± 0.032
0.457TrpAsn: 0.457 ± 0.049
0.812TrpPro: 0.812 ± 0.05
0.551TrpGln: 0.551 ± 0.045
1.422TrpArg: 1.422 ± 0.072
0.921TrpSer: 0.921 ± 0.065
1.016TrpThr: 1.016 ± 0.087
1.059TrpVal: 1.059 ± 0.073
0.283TrpTrp: 0.283 ± 0.034
0.366TrpTyr: 0.366 ± 0.041
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.39TyrAla: 2.39 ± 0.105
0.178TyrCys: 0.178 ± 0.026
1.407TyrAsp: 1.407 ± 0.082
1.317TyrGlu: 1.317 ± 0.069
0.885TyrPhe: 0.885 ± 0.063
2.096TyrGly: 2.096 ± 0.092
0.399TyrHis: 0.399 ± 0.042
0.689TyrIle: 0.689 ± 0.049
0.453TyrLys: 0.453 ± 0.044
2.328TyrLeu: 2.328 ± 0.093
0.301TyrMet: 0.301 ± 0.036
0.453TyrAsn: 0.453 ± 0.047
1.237TyrPro: 1.237 ± 0.075
0.58TyrGln: 0.58 ± 0.051
1.871TyrArg: 1.871 ± 0.087
1.266TyrSer: 1.266 ± 0.077
1.161TyrThr: 1.161 ± 0.081
1.944TyrVal: 1.944 ± 0.094
0.381TyrTrp: 0.381 ± 0.038
0.569TyrTyr: 0.569 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 915 proteins (275724 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski