Capybara microvirus Cap1_SP_94
Average proteome isoelectric point is 5.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7N9|A0A4P8W7N9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_94 OX=2584800 PE=4 SV=1
MM1 pKa = 7.49 FFPFTSYY8 pKa = 11.27 SPDD11 pKa = 3.27 LSGSEE16 pKa = 4.01 VLEE19 pKa = 4.07 RR20 pKa = 11.84 KK21 pKa = 9.69 YY22 pKa = 11.09 DD23 pKa = 3.9 LQTMIDD29 pKa = 4.11 PNNSTLVLHH38 pKa = 7.0 RR39 pKa = 11.84 GLLTDD44 pKa = 3.65 VPISFNSQYY53 pKa = 10.85 LPKK56 pKa = 10.62 PEE58 pKa = 4.34 DD59 pKa = 3.38 TDD61 pKa = 4.9 LAKK64 pKa = 10.4 QLQNGSPLEE73 pKa = 4.7 DD74 pKa = 3.65 YY75 pKa = 10.99 SGSRR79 pKa = 11.84 PFNGAAHH86 pKa = 7.01 HH87 pKa = 6.79 IDD89 pKa = 3.73 DD90 pKa = 4.37 VSANTYY96 pKa = 9.08 MDD98 pKa = 4.31 EE99 pKa = 4.87 LSKK102 pKa = 9.74 TLQTSS107 pKa = 3.39
Molecular weight: 11.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.404
IPC2_protein 4.457
IPC_protein 4.355
Toseland 4.177
ProMoST 4.52
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.279
Rodwell 4.202
Grimsley 4.088
Solomon 4.342
Lehninger 4.291
Nozaki 4.469
DTASelect 4.698
Thurlkill 4.215
EMBOSS 4.291
Sillero 4.482
Patrickios 3.567
IPC_peptide 4.342
IPC2_peptide 4.469
IPC2.peptide.svr19 4.415
Protein with the highest isoelectric point:
>tr|A0A4P8W544|A0A4P8W544_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_94 OX=2584800 PE=4 SV=1
MM1 pKa = 7.73 SICQNFKK8 pKa = 10.7 HH9 pKa = 5.95 IASRR13 pKa = 11.84 SKK15 pKa = 10.69 RR16 pKa = 11.84 LRR18 pKa = 11.84 ANLDD22 pKa = 3.01 ALYY25 pKa = 11.27 NKK27 pKa = 9.32 VSCNKK32 pKa = 9.66 CDD34 pKa = 3.08 SCLAQNRR41 pKa = 11.84 NDD43 pKa = 3.0 WYY45 pKa = 10.1 VRR47 pKa = 11.84 ARR49 pKa = 11.84 FEE51 pKa = 4.11 MMQTLGLDD59 pKa = 3.45 YY60 pKa = 10.82 YY61 pKa = 10.73 NNVVADD67 pKa = 3.97 PGFILFPCLTYY78 pKa = 10.48 IPTNRR83 pKa = 11.84 PTLEE87 pKa = 4.29 LLDD90 pKa = 5.37 DD91 pKa = 4.32 NSLPYY96 pKa = 10.41 YY97 pKa = 10.66 FEE99 pKa = 4.49 GFSYY103 pKa = 10.59 SHH105 pKa = 7.06 IKK107 pKa = 10.34 RR108 pKa = 11.84 FLVEE112 pKa = 4.33 LADD115 pKa = 3.82 KK116 pKa = 9.98 FQNRR120 pKa = 11.84 YY121 pKa = 7.61 HH122 pKa = 6.84 TGTSCGVRR130 pKa = 11.84 YY131 pKa = 8.46 MVASEE136 pKa = 3.77 YY137 pKa = 11.07 GYY139 pKa = 11.27 AEE141 pKa = 4.78 EE142 pKa = 4.25 YY143 pKa = 10.98 LDD145 pKa = 5.34 DD146 pKa = 5.04 FGHH149 pKa = 6.37 SRR151 pKa = 11.84 IGQAAPHH158 pKa = 4.95 YY159 pKa = 9.69 HH160 pKa = 5.85 VLLFFPSVAYY170 pKa = 10.23 NDD172 pKa = 3.36 KK173 pKa = 10.97 DD174 pKa = 3.38 YY175 pKa = 10.74 WADD178 pKa = 3.39 VVEE181 pKa = 4.65 SLWSVTHH188 pKa = 4.55 RR189 pKa = 11.84 HH190 pKa = 5.57 GRR192 pKa = 11.84 VGWSKK197 pKa = 10.69 KK198 pKa = 9.07 HH199 pKa = 5.4 GKK201 pKa = 9.44 FVTSPLAIKK210 pKa = 9.16 YY211 pKa = 5.33 TTQYY215 pKa = 10.16 CHH217 pKa = 6.53 KK218 pKa = 9.46 QHH220 pKa = 6.74 SYY222 pKa = 7.52 YY223 pKa = 10.24 QRR225 pKa = 11.84 PEE227 pKa = 3.64 IEE229 pKa = 3.49 KK230 pKa = 9.78 WLYY233 pKa = 10.58 QDD235 pKa = 3.29 ITKK238 pKa = 8.14 DD239 pKa = 3.52 TPFHH243 pKa = 5.72 VQHH246 pKa = 6.29 EE247 pKa = 4.63 LIKK250 pKa = 10.47 EE251 pKa = 4.07 RR252 pKa = 11.84 EE253 pKa = 4.11 KK254 pKa = 10.86 QIHH257 pKa = 7.4 DD258 pKa = 3.31 ILPFHH263 pKa = 7.23 RR264 pKa = 11.84 EE265 pKa = 3.69 SLHH268 pKa = 7.05 FGEE271 pKa = 5.38 CAVDD275 pKa = 4.0 FLKK278 pKa = 11.04 SLSSEE283 pKa = 3.9 EE284 pKa = 4.15 RR285 pKa = 11.84 IKK287 pKa = 11.09 LLSNGITFPDD297 pKa = 4.07 DD298 pKa = 3.3 FAADD302 pKa = 3.67 GTLNHH307 pKa = 6.88 YY308 pKa = 10.15 ILPKK312 pKa = 10.23 YY313 pKa = 10.33 IYY315 pKa = 9.78 DD316 pKa = 3.4 KK317 pKa = 11.09 LYY319 pKa = 10.58 FVIEE323 pKa = 4.16 NKK325 pKa = 10.42 SVIEE329 pKa = 4.35 DD330 pKa = 3.43 GKK332 pKa = 11.02 SVVKK336 pKa = 10.07 PFVRR340 pKa = 11.84 YY341 pKa = 9.92 GSDD344 pKa = 3.27 YY345 pKa = 11.65 ALADD349 pKa = 3.35 YY350 pKa = 7.5 RR351 pKa = 11.84 TLFRR355 pKa = 11.84 YY356 pKa = 9.22 NFNKK360 pKa = 10.11 SRR362 pKa = 11.84 FDD364 pKa = 3.72 LEE366 pKa = 5.28 RR367 pKa = 11.84 ITSNLFLSQLDD378 pKa = 3.56 KK379 pKa = 11.47 EE380 pKa = 4.95 LIEE383 pKa = 4.45 KK384 pKa = 10.13 LNKK387 pKa = 9.16 RR388 pKa = 11.84 DD389 pKa = 3.66 YY390 pKa = 10.6 VLQYY394 pKa = 10.17 NYY396 pKa = 11.17 ADD398 pKa = 3.56 FRR400 pKa = 11.84 HH401 pKa = 6.53 FIDD404 pKa = 5.65 DD405 pKa = 3.14 IKK407 pKa = 11.32 SRR409 pKa = 11.84 YY410 pKa = 7.3 TFDD413 pKa = 5.63 RR414 pKa = 11.84 ILSYY418 pKa = 10.96 KK419 pKa = 10.3 HH420 pKa = 6.13 IVHH423 pKa = 6.71 HH424 pKa = 6.63 LFAFDD429 pKa = 3.95 PLSYY433 pKa = 11.1 SDD435 pKa = 4.92 DD436 pKa = 3.57 DD437 pKa = 4.52 LYY439 pKa = 11.96 NFVEE443 pKa = 5.13 DD444 pKa = 3.44 ISVNKK449 pKa = 10.13 ILTKK453 pKa = 10.43 RR454 pKa = 11.84 FSSLDD459 pKa = 3.33 KK460 pKa = 11.23 YY461 pKa = 10.82 EE462 pKa = 3.92 LSIYY466 pKa = 10.29 QKK468 pKa = 10.88 LFSSYY473 pKa = 10.17 NSPDD477 pKa = 2.91 ISYY480 pKa = 10.9 LDD482 pKa = 4.28 GYY484 pKa = 10.75 SCVRR488 pKa = 11.84 NDD490 pKa = 3.89 EE491 pKa = 4.17 LDD493 pKa = 3.54 EE494 pKa = 4.34 FLSIIDD500 pKa = 4.24 TIKK503 pKa = 10.62 LHH505 pKa = 7.11 ASVHH509 pKa = 5.53 RR510 pKa = 11.84 KK511 pKa = 8.77 KK512 pKa = 10.8 AYY514 pKa = 10.36 DD515 pKa = 3.66 LVCLQKK521 pKa = 10.3 QAHH524 pKa = 7.17 KK525 pKa = 10.57 YY526 pKa = 9.84 RR527 pKa = 11.84 NLSKK531 pKa = 11.2 YY532 pKa = 10.22 ILL534 pKa = 3.91
Molecular weight: 63.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.629
IPC2_protein 6.737
IPC_protein 6.795
Toseland 6.898
ProMoST 7.278
Dawson 7.322
Bjellqvist 7.337
Wikipedia 7.293
Rodwell 7.322
Grimsley 7.029
Solomon 7.337
Lehninger 7.351
Nozaki 7.6
DTASelect 7.614
Thurlkill 7.644
EMBOSS 7.658
Sillero 7.732
Patrickios 3.668
IPC_peptide 7.351
IPC2_peptide 7.146
IPC2.peptide.svr19 7.018
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1658
107
668
414.5
47.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.514 ± 1.424
1.086 ± 0.392
7.057 ± 0.594
4.524 ± 0.944
5.127 ± 1.063
5.127 ± 0.766
2.473 ± 0.84
6.152 ± 0.562
5.489 ± 0.931
9.409 ± 0.742
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.87 ± 0.438
5.368 ± 0.439
3.739 ± 0.882
4.222 ± 0.559
4.584 ± 0.469
9.59 ± 0.631
5.79 ± 0.891
5.006 ± 0.245
1.086 ± 0.231
5.79 ± 1.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here