Rhinolophus ferrumequinum (Greater horseshoe bat)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Chiroptera;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33504 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A671G0U1|A0A671G0U1_RHIFE Negative elongation factor complex member C/D OS=Rhinolophus ferrumequinum OX=59479 GN=NELFCD PE=3 SV=1
MM1 pKa = 7.18EE2 pKa = 5.16AAVLLSLVLGPLLLPLLAVLLMALCVRR29 pKa = 11.84CRR31 pKa = 11.84EE32 pKa = 4.42LPDD35 pKa = 4.46SYY37 pKa = 11.74DD38 pKa = 3.71SVPSDD43 pKa = 3.41SLTPSSIMIKK53 pKa = 10.1RR54 pKa = 11.84PPTLAPWPPATSYY67 pKa = 11.11PPVTSYY73 pKa = 11.28PPLSQPDD80 pKa = 4.23LLPIPRR86 pKa = 11.84SPQPPGGSHH95 pKa = 6.82RR96 pKa = 11.84MPSSRR101 pKa = 11.84QDD103 pKa = 2.92SDD105 pKa = 3.47GANSVASYY113 pKa = 10.76EE114 pKa = 4.13NEE116 pKa = 3.97EE117 pKa = 4.44PVCEE121 pKa = 5.56DD122 pKa = 3.8DD123 pKa = 6.81DD124 pKa = 4.57EE125 pKa = 7.31DD126 pKa = 4.47EE127 pKa = 6.03DD128 pKa = 4.74EE129 pKa = 4.45EE130 pKa = 5.69DD131 pKa = 3.77YY132 pKa = 11.6HH133 pKa = 8.47NEE135 pKa = 4.01GYY137 pKa = 11.0LEE139 pKa = 4.13VLPDD143 pKa = 3.72TAPATGTAVPAAPVPSNPGLRR164 pKa = 11.84DD165 pKa = 3.14SAFSMEE171 pKa = 4.23SGEE174 pKa = 5.27DD175 pKa = 3.49YY176 pKa = 11.54VNYY179 pKa = 9.95PEE181 pKa = 5.37SEE183 pKa = 4.17GSAQASLDD191 pKa = 3.4GSRR194 pKa = 11.84EE195 pKa = 3.99YY196 pKa = 11.72VNVSQEE202 pKa = 4.01LQPTSRR208 pKa = 11.84TKK210 pKa = 9.72TAALSSQEE218 pKa = 4.35AEE220 pKa = 4.32DD221 pKa = 4.33EE222 pKa = 4.38EE223 pKa = 5.29EE224 pKa = 4.1EE225 pKa = 4.75AAPDD229 pKa = 3.85YY230 pKa = 11.47EE231 pKa = 4.23NLL233 pKa = 3.68

Molecular weight: 25.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A671EGY4|A0A671EGY4_RHIFE Uncharacterized protein OS=Rhinolophus ferrumequinum OX=59479 GN=mRhiFer1_003603 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19443

14061

33504

19587673

13

35440

584.6

65.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.997 ± 0.015

2.151 ± 0.01

4.877 ± 0.009

7.167 ± 0.019

3.582 ± 0.01

6.515 ± 0.018

2.556 ± 0.009

4.332 ± 0.013

5.8 ± 0.022

9.846 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.139 ± 0.007

3.62 ± 0.009

6.314 ± 0.023

4.768 ± 0.015

5.689 ± 0.012

8.382 ± 0.019

5.356 ± 0.013

6.086 ± 0.017

1.189 ± 0.005

2.633 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski