Synechococcus virus Syn5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Voetvirus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4ZR95|A4ZR95_9CAUD Gp14 OS=Synechococcus virus Syn5 OX=438482 GN=14 PE=4 SV=1
MM1 pKa = 7.57NYY3 pKa = 10.31SSDD6 pKa = 3.3FTYY9 pKa = 11.07YY10 pKa = 10.84LQDD13 pKa = 4.55LSDD16 pKa = 4.81ADD18 pKa = 4.39LADD21 pKa = 5.16LYY23 pKa = 10.82VQYY26 pKa = 10.75IEE28 pKa = 4.79RR29 pKa = 11.84RR30 pKa = 11.84NTAWFSDD37 pKa = 3.67IFWEE41 pKa = 4.21ANRR44 pKa = 11.84RR45 pKa = 11.84GLTLDD50 pKa = 3.42EE51 pKa = 5.18LEE53 pKa = 6.02GIHH56 pKa = 5.77QQQFDD61 pKa = 4.56PPCSLDD67 pKa = 3.34SS68 pKa = 3.8

Molecular weight: 8.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4ZR93|A4ZR93_9CAUD Gp12 OS=Synechococcus virus Syn5 OX=438482 GN=12 PE=4 SV=1
MM1 pKa = 7.61NILTIIRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.62ALKK13 pKa = 10.26AQAKK17 pKa = 8.86YY18 pKa = 10.26KK19 pKa = 10.56ALRR22 pKa = 11.84AQFLKK27 pKa = 10.97ANEE30 pKa = 3.98ITGG33 pKa = 3.55

Molecular weight: 3.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

14534

30

1415

238.3

26.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.245 ± 0.503

0.839 ± 0.15

6.179 ± 0.275

5.952 ± 0.397

3.887 ± 0.15

7.555 ± 0.416

1.672 ± 0.215

4.864 ± 0.198

4.617 ± 0.428

8.532 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.112 ± 0.231

4.844 ± 0.453

4.032 ± 0.171

4.692 ± 0.289

5.043 ± 0.386

7.149 ± 0.311

7.094 ± 0.546

6.406 ± 0.398

1.197 ± 0.123

3.089 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski