Vibrio sp. V15_P4S5T153

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; unclassified Vibrio

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4788 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A233HUU0|A0A233HUU0_9VIBR Uncharacterized protein OS=Vibrio sp. V15_P4S5T153 OX=1938669 GN=B9J89_04680 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.38KK3 pKa = 10.28VLLTLAVSAAFSSQAFSATTFDD25 pKa = 3.53ARR27 pKa = 11.84SVGMGGIGVSTSNYY41 pKa = 10.02LMAPFHH47 pKa = 6.44NPALVAKK54 pKa = 9.97YY55 pKa = 10.49GDD57 pKa = 3.8SDD59 pKa = 4.11DD60 pKa = 4.8VGILLPSIGVNLQDD74 pKa = 3.75KK75 pKa = 11.24GEE77 pKa = 4.13MVDD80 pKa = 4.96GIDD83 pKa = 4.05SAVDD87 pKa = 4.0AIDD90 pKa = 4.0ALEE93 pKa = 4.32ANRR96 pKa = 11.84TQQNASNVVDD106 pKa = 5.09ALAALNRR113 pKa = 11.84DD114 pKa = 3.21SAYY117 pKa = 9.64VQAGVGMAVAIPNQLVSVNLFTQGYY142 pKa = 9.52ADD144 pKa = 3.63AFVYY148 pKa = 10.93ADD150 pKa = 3.94VADD153 pKa = 3.99SDD155 pKa = 4.71LNEE158 pKa = 4.42SEE160 pKa = 4.98LLNPSHH166 pKa = 6.94TYY168 pKa = 11.11DD169 pKa = 3.04STATVAGIAVVEE181 pKa = 5.33FGAALAKK188 pKa = 10.05EE189 pKa = 4.32YY190 pKa = 10.8KK191 pKa = 10.33LEE193 pKa = 4.16NSVIYY198 pKa = 10.74YY199 pKa = 10.72GLTPKK204 pKa = 9.91IQQVKK209 pKa = 8.03TINYY213 pKa = 8.67VADD216 pKa = 3.38INNFEE221 pKa = 4.47FDD223 pKa = 5.62DD224 pKa = 4.06MTDD227 pKa = 3.28DD228 pKa = 3.8QYY230 pKa = 12.14QNSEE234 pKa = 3.95TSFNLDD240 pKa = 2.69AGVAYY245 pKa = 9.57TMNNGLSLGLVGRR258 pKa = 11.84NLISQSYY265 pKa = 8.38DD266 pKa = 3.12TVEE269 pKa = 3.95LDD271 pKa = 3.72GVKK274 pKa = 10.34ASYY277 pKa = 10.4SINPVFVASASYY289 pKa = 9.62NYY291 pKa = 10.76SWFTVGADD299 pKa = 3.0IEE301 pKa = 4.54LNEE304 pKa = 4.24TEE306 pKa = 4.67RR307 pKa = 11.84FEE309 pKa = 5.47DD310 pKa = 3.07MSGFMNSIDD319 pKa = 3.64SSSDD323 pKa = 3.0NTQLAGIGIEE333 pKa = 4.08LNAWDD338 pKa = 4.08WAQLRR343 pKa = 11.84AGYY346 pKa = 8.02QTDD349 pKa = 3.15IANNLDD355 pKa = 3.56DD356 pKa = 4.85QFTAGIGLSPFGTVRR371 pKa = 11.84IDD373 pKa = 3.62LAGSYY378 pKa = 10.24AGEE381 pKa = 4.1NQLGVSLQTSMTFF394 pKa = 3.14

Molecular weight: 42.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A233HQP0|A0A233HQP0_9VIBR Uncharacterized protein OS=Vibrio sp. V15_P4S5T153 OX=1938669 GN=B9J89_06075 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.99RR4 pKa = 11.84TFQPTVLKK12 pKa = 10.46RR13 pKa = 11.84KK14 pKa = 7.65RR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.44NGRR29 pKa = 11.84KK30 pKa = 9.39VINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.22GRR40 pKa = 11.84ARR42 pKa = 11.84LSKK45 pKa = 10.83

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4788

0

4788

1438149

19

4401

300.4

33.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.278 ± 0.043

1.112 ± 0.012

5.417 ± 0.029

6.417 ± 0.039

4.182 ± 0.025

6.436 ± 0.037

2.354 ± 0.019

6.535 ± 0.029

5.717 ± 0.038

10.368 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.019

4.443 ± 0.026

3.693 ± 0.025

4.686 ± 0.037

4.496 ± 0.028

6.835 ± 0.032

5.171 ± 0.022

6.821 ± 0.03

1.206 ± 0.015

3.174 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski