Rothia aeria

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Rothia

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2797 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5QYD6|A0A2Z5QYD6_9MICC Uncharacterized protein OS=Rothia aeria OX=172042 GN=RA11412_0955 PE=4 SV=1
MM1 pKa = 7.4CLEE4 pKa = 5.35LEE6 pKa = 4.48VQDD9 pKa = 4.65ATATYY14 pKa = 11.08NDD16 pKa = 5.34LKK18 pKa = 10.23TQGLDD23 pKa = 2.72ITYY26 pKa = 9.56PLTDD30 pKa = 3.13EE31 pKa = 5.35PYY33 pKa = 7.82GQRR36 pKa = 11.84RR37 pKa = 11.84FGFSDD42 pKa = 3.99PSGLWVDD49 pKa = 3.06IVEE52 pKa = 4.53QIEE55 pKa = 4.09PAAGYY60 pKa = 7.8WDD62 pKa = 4.84KK63 pKa = 12.0YY64 pKa = 9.23MLPP67 pKa = 3.96

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5QWJ7|A0A2Z5QWJ7_9MICC Uncharacterized protein OS=Rothia aeria OX=172042 GN=RA11412_0451 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.87GRR40 pKa = 11.84AKK42 pKa = 10.73LSAA45 pKa = 3.92

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2797

0

2797

684140

37

1572

244.6

26.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.371 ± 0.071

0.82 ± 0.015

5.181 ± 0.043

6.258 ± 0.055

3.269 ± 0.04

8.137 ± 0.053

2.266 ± 0.03

4.872 ± 0.045

3.614 ± 0.048

9.336 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.024

3.172 ± 0.036

5.306 ± 0.048

3.601 ± 0.046

6.252 ± 0.056

6.253 ± 0.051

6.286 ± 0.041

7.569 ± 0.047

1.345 ± 0.019

2.811 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski