Haloterrigena sp. YPL8
Average proteome isoelectric point is 4.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3981 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D5GNG8|A0A7D5GNG8_9EURY Uncharacterized protein OS=Haloterrigena sp. YPL8 OX=1699371 GN=HYG82_11000 PE=4 SV=1
MM1 pKa = 6.59 VTTGAWGGFHH11 pKa = 7.67 RR12 pKa = 11.84 MGCITRR18 pKa = 11.84 RR19 pKa = 11.84 DD20 pKa = 3.48 ILKK23 pKa = 10.38 ASLGFGTVMGVGLVGRR39 pKa = 11.84 WPEE42 pKa = 4.1 TTTSEE47 pKa = 4.19 TSFGDD52 pKa = 3.83 GVNLQPSYY60 pKa = 10.89 FCSANQDD67 pKa = 3.39 IGWDD71 pKa = 3.85 FMSQYY76 pKa = 11.42 SDD78 pKa = 2.75 IQTLRR83 pKa = 11.84 MRR85 pKa = 11.84 IEE87 pKa = 4.06 PFSFSEE93 pKa = 3.91 VDD95 pKa = 3.47 TTVEE99 pKa = 4.26 DD100 pKa = 3.52 AKK102 pKa = 10.89 RR103 pKa = 11.84 WIDD106 pKa = 3.36 EE107 pKa = 4.17 ATEE110 pKa = 3.77 NGYY113 pKa = 10.35 DD114 pKa = 3.63 VIASYY119 pKa = 10.32 HH120 pKa = 6.51 HH121 pKa = 6.52 YY122 pKa = 9.36 PDD124 pKa = 4.38 NGSSQASALQHH135 pKa = 6.79 AADD138 pKa = 4.69 FWVEE142 pKa = 3.86 HH143 pKa = 6.43 YY144 pKa = 7.72 GTLSQDD150 pKa = 3.25 SAFTINLMNEE160 pKa = 3.94 WGDD163 pKa = 3.91 HH164 pKa = 5.12 NVTASAYY171 pKa = 9.79 ASAYY175 pKa = 10.11 NDD177 pKa = 4.36 AIDD180 pKa = 3.89 TVRR183 pKa = 11.84 SGTSYY188 pKa = 11.06 DD189 pKa = 3.64 GEE191 pKa = 4.49 IVCDD195 pKa = 3.73 APGWGQGTHH204 pKa = 6.47 RR205 pKa = 11.84 LADD208 pKa = 3.59 AVEE211 pKa = 4.84 EE212 pKa = 4.36 IDD214 pKa = 6.11 DD215 pKa = 4.71 DD216 pKa = 4.75 DD217 pKa = 5.1 LVLSVHH223 pKa = 6.51 VYY225 pKa = 9.71 PNAYY229 pKa = 9.96 NATTGEE235 pKa = 4.16 WLRR238 pKa = 11.84 PEE240 pKa = 4.29 HH241 pKa = 7.01 LDD243 pKa = 4.07 VIDD246 pKa = 3.72 EE247 pKa = 4.26 TGYY250 pKa = 9.86 PCMIGEE256 pKa = 4.55 FGKK259 pKa = 10.59 YY260 pKa = 7.64 WPTQYY265 pKa = 11.36 SDD267 pKa = 3.18 TADD270 pKa = 4.2 ADD272 pKa = 3.39 WLAIVDD278 pKa = 4.09 HH279 pKa = 6.32 ATSLGWPVIGWAWNGDD295 pKa = 3.63 TNEE298 pKa = 3.72 QRR300 pKa = 11.84 MNMVEE305 pKa = 4.5 PYY307 pKa = 9.87 WDD309 pKa = 4.22 DD310 pKa = 3.57 EE311 pKa = 4.64 CGGPYY316 pKa = 9.71 SEE318 pKa = 5.15 SSYY321 pKa = 11.22 FDD323 pKa = 3.41 VIYY326 pKa = 10.53 EE327 pKa = 4.09 KK328 pKa = 10.84 LGSGGNDD335 pKa = 2.93 TGRR338 pKa = 11.84 NDD340 pKa = 5.25 DD341 pKa = 5.48 DD342 pKa = 5.04 GDD344 pKa = 4.07 EE345 pKa = 4.42 NGGNSEE351 pKa = 3.83 PTAAIDD357 pKa = 4.5 ADD359 pKa = 3.81 ATDD362 pKa = 3.86 VSVGDD367 pKa = 4.05 TPSFDD372 pKa = 3.72 ASASSEE378 pKa = 3.81 EE379 pKa = 4.35 DD380 pKa = 3.22 GTIEE384 pKa = 4.17 RR385 pKa = 11.84 YY386 pKa = 8.96 EE387 pKa = 3.91 WTFGDD392 pKa = 4.09 GTSATGKK399 pKa = 10.09 RR400 pKa = 11.84 VDD402 pKa = 3.77 HH403 pKa = 6.53 AFEE406 pKa = 4.4 EE407 pKa = 4.76 PGEE410 pKa = 4.16 YY411 pKa = 9.23 TVLLTVTDD419 pKa = 4.77 DD420 pKa = 3.91 EE421 pKa = 4.85 NATDD425 pKa = 3.71 TDD427 pKa = 4.54 TITVTVTDD435 pKa = 4.08 SAKK438 pKa = 10.29 SAPGAPANLTVVATTTSSLTIEE460 pKa = 4.05 WDD462 pKa = 3.69 GVDD465 pKa = 3.48 DD466 pKa = 4.32 ADD468 pKa = 4.24 YY469 pKa = 11.3 YY470 pKa = 10.78 VVSVDD475 pKa = 3.44 GSVDD479 pKa = 3.2 HH480 pKa = 6.4 EE481 pKa = 4.66 TPEE484 pKa = 4.04 TTATIDD490 pKa = 3.47 EE491 pKa = 5.09 LEE493 pKa = 4.33 TEE495 pKa = 4.14 TGYY498 pKa = 11.04 EE499 pKa = 3.92 IGVSAVDD506 pKa = 3.27 NGGAEE511 pKa = 4.4 SATKK515 pKa = 9.43 TVSATTASDD524 pKa = 3.67 DD525 pKa = 4.64 GSDD528 pKa = 4.26 EE529 pKa = 4.63 DD530 pKa = 6.22 DD531 pKa = 3.47 EE532 pKa = 5.74 DD533 pKa = 4.59 RR534 pKa = 11.84 GNGDD538 pKa = 3.2 FDD540 pKa = 5.37 GDD542 pKa = 3.71 LVAEE546 pKa = 4.68 IRR548 pKa = 11.84 ASTTSAWVGEE558 pKa = 4.48 HH559 pKa = 6.18 IGFFAVDD566 pKa = 3.5 RR567 pKa = 11.84 TGDD570 pKa = 3.86 DD571 pKa = 3.13 TWPTEE576 pKa = 4.07 LDD578 pKa = 2.99 WDD580 pKa = 4.47 LGDD583 pKa = 3.97 GTTASGWYY591 pKa = 7.37 TAYY594 pKa = 10.39 AYY596 pKa = 8.85 DD597 pKa = 3.76 SPGTYY602 pKa = 8.69 TVALTATDD610 pKa = 4.41 DD611 pKa = 3.62 EE612 pKa = 5.22 GTTTTHH618 pKa = 5.51 EE619 pKa = 3.94 VEE621 pKa = 3.83 IAVFRR626 pKa = 11.84 VRR628 pKa = 11.84 RR629 pKa = 11.84 SSPAVRR635 pKa = 11.84 SSS637 pKa = 2.74
Molecular weight: 68.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.719
IPC_protein 3.77
Toseland 3.541
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.439
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.884
Patrickios 1.138
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A7D5GL18|A0A7D5GL18_9EURY Arginine decarboxylase OS=Haloterrigena sp. YPL8 OX=1699371 GN=HYG82_11880 PE=3 SV=1
MM1 pKa = 7.57 ARR3 pKa = 11.84 RR4 pKa = 11.84 AVARR8 pKa = 11.84 YY9 pKa = 9.38 SSTFSKK15 pKa = 10.36 RR16 pKa = 11.84 RR17 pKa = 11.84 YY18 pKa = 7.54 TLHH21 pKa = 4.79 QHH23 pKa = 7.27 IILLCLTVRR32 pKa = 11.84 KK33 pKa = 8.33 NTTYY37 pKa = 11.35 RR38 pKa = 11.84 MLLDD42 pKa = 4.22 EE43 pKa = 5.71 LIEE46 pKa = 3.96 MPRR49 pKa = 11.84 IRR51 pKa = 11.84 RR52 pKa = 11.84 AIEE55 pKa = 4.03 LEE57 pKa = 4.05 EE58 pKa = 4.56 LPSPSTLCKK67 pKa = 10.35 AFNRR71 pKa = 11.84 LGMAVWRR78 pKa = 11.84 ILLTLSVTLLPTNGVVGIDD97 pKa = 3.05 ASGFDD102 pKa = 3.53 RR103 pKa = 11.84 SHH105 pKa = 6.94 ASKK108 pKa = 10.5 HH109 pKa = 3.65 YY110 pKa = 8.79 TKK112 pKa = 9.81 RR113 pKa = 11.84 TKK115 pKa = 9.96 LTIQQLTVTLLVDD128 pKa = 3.67 TRR130 pKa = 11.84 ANAILDD136 pKa = 3.56 LHH138 pKa = 5.57 VTTTRR143 pKa = 11.84 THH145 pKa = 6.9 DD146 pKa = 3.68 SKK148 pKa = 10.95 IAPSLSKK155 pKa = 10.73 RR156 pKa = 11.84 NSGEE160 pKa = 3.76 VAIFLGDD167 pKa = 4.35 KK168 pKa = 10.91 GYY170 pKa = 10.96 DD171 pKa = 3.48 DD172 pKa = 4.37 QKK174 pKa = 10.85 IHH176 pKa = 6.93 ALAHH180 pKa = 6.05 EE181 pKa = 5.01 DD182 pKa = 3.73 GVRR185 pKa = 11.84 PLIKK189 pKa = 10.1 HH190 pKa = 6.19 RR191 pKa = 11.84 EE192 pKa = 3.73 FSSLHH197 pKa = 5.64 KK198 pKa = 10.65 ARR200 pKa = 11.84 NARR203 pKa = 11.84 LDD205 pKa = 3.64 ADD207 pKa = 3.98 SYY209 pKa = 9.04 GHH211 pKa = 5.2 VVRR214 pKa = 11.84 TRR216 pKa = 11.84 RR217 pKa = 11.84 RR218 pKa = 11.84 TLASNGNTAHH228 pKa = 7.0 SSAHH232 pKa = 5.68 DD233 pKa = 3.57 TGGSSSVNSLSAVSLTPSTRR253 pKa = 11.84 HH254 pKa = 5.97 PEE256 pKa = 3.74 RR257 pKa = 5.77
Molecular weight: 28.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.633
IPC_protein 10.409
Toseland 10.657
ProMoST 10.467
Dawson 10.745
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 10.965
Grimsley 10.804
Solomon 10.862
Lehninger 10.833
Nozaki 10.628
DTASelect 10.452
Thurlkill 10.643
EMBOSS 11.052
Sillero 10.672
Patrickios 10.657
IPC_peptide 10.862
IPC2_peptide 9.355
IPC2.peptide.svr19 8.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3981
0
3981
1144613
42
1563
287.5
31.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.052 ± 0.055
0.766 ± 0.012
8.5 ± 0.052
8.601 ± 0.065
3.335 ± 0.028
8.351 ± 0.045
2.007 ± 0.02
4.724 ± 0.028
1.905 ± 0.021
8.698 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.754 ± 0.015
2.601 ± 0.022
4.556 ± 0.028
2.56 ± 0.024
6.511 ± 0.035
5.947 ± 0.035
6.7 ± 0.03
8.504 ± 0.041
1.18 ± 0.016
2.751 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here