Proteome-pI 2.0 - Proteome Isoelectric Point Database

Wallemia mellicola (strain ATCC MYA-4683 / CBS 633.66) (Wallemia sebi (CBS 633.66))

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Wallemiomycotina; Wallemiomycetes; Wallemiales; Wallemiaceae; Wallemia; Wallemia mellicola

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 5262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|I4Y6E8|I4Y6E8_WALMC p-loop containing nucleoside triphosphate hydrolase protein OS=Wallemia mellicola (strain ATCC MYA-4683 / CBS 633.66) OX=671144 GN=WALSEDRAFT_61439 PE=4 SV=1

MM1 pKa = 7.22QFLTILSLATAAFAASVKK19 pKa = 10.26RR20 pKa = 11.84DD21 pKa = 3.21VFTDD25 pKa = 3.31SSKK28 pKa = 10.64FNQLISSPEE37 pKa = 3.89LEE39 pKa = 3.52QCYY42 pKa = 10.14QNQCLPIRR50 pKa = 11.84EE51 pKa = 4.36KK52 pKa = 10.99LRR54 pKa = 11.84SDD56 pKa = 4.16CLPIIQNTDD65 pKa = 2.55INSIDD70 pKa = 3.64TEE72 pKa = 4.41LGFEE76 pKa = 4.78FGNCVCEE83 pKa = 4.63GFDD86 pKa = 4.27FAQQNPEE93 pKa = 4.92CGDD96 pKa = 3.83CMNDD100 pKa = 4.15AIDD103 pKa = 4.16NLTSEE108 pKa = 5.51LDD110 pKa = 3.58QTCTAIRR117 pKa = 11.84SDD119 pKa = 3.62PSNPEE124 pKa = 3.68NDD126 pKa = 3.85DD127 pKa = 3.5SSSITNIPPASLLASSLLVVGISFII152 pKa = 4.29

Molecular weight: 16.58 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|I4Y7Z9|I4Y7Z9_WALMC Uncharacterized protein OS=Wallemia mellicola (strain ATCC MYA-4683 / CBS 633.66) OX=671144 GN=WALSEDRAFT_65674 PE=4 SV=1

MM1 pKa = 7.49PAAPPGRR8 pKa = 11.84STPAAPPARR17 pKa = 11.84ATPPPPARR25 pKa = 11.84GTPVPPAPPASSTPPARR42 pKa = 11.84TTPPAPPIGRR52 pKa = 11.84LPPPVPPVAAAPTKK66 pKa = 10.31KK67 pKa = 9.99PPPPAPKK74 pKa = 9.86PKK76 pKa = 9.64PKK78 pKa = 9.93PPAPAAPPAPAAPPSQPPRR97 pKa = 11.84VLPPGPPPGQPARR110 pKa = 11.84SIPPPPPSRR119 pKa = 11.84PSPPPAPQRR128 pKa = 11.84NAPPSLPSRR137 pKa = 11.84TPSTASS143 pKa = 2.97

Molecular weight: 13.96 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
5260	2	5262	2223108	49	4930	422.5	47.34

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.031 ± 0.031	1.041 ± 0.012	6.144 ± 0.028	6.522 ± 0.034	3.848 ± 0.022	5.658 ± 0.033	2.277 ± 0.016	6.019 ± 0.025	6.365 ± 0.035	9.353 ± 0.038

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.016 ± 0.012	5.043 ± 0.025	4.919 ± 0.03	4.279 ± 0.029	5.191 ± 0.025	8.56 ± 0.034	5.704 ± 0.018	5.828 ± 0.025	1.139 ± 0.011	3.065 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski