Canis familiaris papillomavirus 13
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7JLS2|J7JLS2_9PAPI Protein E6 OS=Canis familiaris papillomavirus 13 OX=1226723 GN=E6 PE=3 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 GLAPTNRR9 pKa = 11.84 DD10 pKa = 2.86 LDD12 pKa = 4.11 LEE14 pKa = 4.53 LGEE17 pKa = 5.31 LVLPEE22 pKa = 4.37 NLLSSEE28 pKa = 4.11 RR29 pKa = 11.84 LDD31 pKa = 3.73 TEE33 pKa = 4.4 EE34 pKa = 5.1 EE35 pKa = 4.05 EE36 pKa = 4.98 SEE38 pKa = 4.7 PDD40 pKa = 3.42 PQHH43 pKa = 5.82 YY44 pKa = 9.53 RR45 pKa = 11.84 VVTNCGRR52 pKa = 11.84 CHH54 pKa = 4.84 STLRR58 pKa = 11.84 VFVAVLSVFQLRR70 pKa = 11.84 TFEE73 pKa = 4.28 QLLLDD78 pKa = 4.6 GFTIVCLRR86 pKa = 11.84 CSQQQSHH93 pKa = 5.88 GRR95 pKa = 11.84 HH96 pKa = 5.26
Molecular weight: 10.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.978
IPC2_protein 5.067
IPC_protein 4.94
Toseland 4.914
ProMoST 4.978
Dawson 4.94
Bjellqvist 5.067
Wikipedia 4.8
Rodwell 4.863
Grimsley 4.838
Solomon 4.927
Lehninger 4.889
Nozaki 5.067
DTASelect 5.169
Thurlkill 4.914
EMBOSS 4.851
Sillero 5.143
Patrickios 3.859
IPC_peptide 4.94
IPC2_peptide 5.143
IPC2.peptide.svr19 5.078
Protein with the highest isoelectric point:
>tr|J7JMM7|J7JMM7_9PAPI Minor capsid protein L2 OS=Canis familiaris papillomavirus 13 OX=1226723 GN=L2 PE=3 SV=1
MM1 pKa = 7.65 EE2 pKa = 5.0 NLHH5 pKa = 5.89 TRR7 pKa = 11.84 FDD9 pKa = 3.94 VVQEE13 pKa = 3.98 VLFQHH18 pKa = 6.29 YY19 pKa = 8.74 EE20 pKa = 3.94 QGSHH24 pKa = 6.76 KK25 pKa = 10.68 LSDD28 pKa = 3.39 HH29 pKa = 6.09 ALFWEE34 pKa = 4.79 AKK36 pKa = 8.4 RR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 4.15 AIMLFFARR47 pKa = 11.84 KK48 pKa = 8.68 RR49 pKa = 11.84 QMPRR53 pKa = 11.84 LGFQPVPALSVSEE66 pKa = 5.0 GNAKK70 pKa = 10.24 DD71 pKa = 3.94 AIKK74 pKa = 10.15 MGLLLSSLQKK84 pKa = 10.5 SPYY87 pKa = 9.85 GDD89 pKa = 3.79 EE90 pKa = 3.93 PWRR93 pKa = 11.84 ITDD96 pKa = 3.65 VSLEE100 pKa = 3.99 MLNTEE105 pKa = 5.16 PKK107 pKa = 10.71 DD108 pKa = 3.71 CFKK111 pKa = 11.37 KK112 pKa = 10.58 NGITVEE118 pKa = 3.8 VHH120 pKa = 6.25 YY121 pKa = 11.39 DD122 pKa = 3.51 NDD124 pKa = 3.99 PDD126 pKa = 3.76 NAAHH130 pKa = 5.04 YY131 pKa = 7.14 TSWSEE136 pKa = 3.8 IYY138 pKa = 9.52 YY139 pKa = 10.66 QNVDD143 pKa = 3.78 DD144 pKa = 5.17 DD145 pKa = 4.15 WVKK148 pKa = 10.22 VRR150 pKa = 11.84 GRR152 pKa = 11.84 VNYY155 pKa = 9.74 EE156 pKa = 3.32 GLFFVDD162 pKa = 3.28 EE163 pKa = 4.98 DD164 pKa = 4.16 GEE166 pKa = 3.95 EE167 pKa = 4.0 RR168 pKa = 11.84 YY169 pKa = 9.65 YY170 pKa = 11.61 VRR172 pKa = 11.84 FQKK175 pKa = 10.64 DD176 pKa = 2.98 AEE178 pKa = 4.43 RR179 pKa = 11.84 YY180 pKa = 9.04 GVTGMWRR187 pKa = 11.84 VHH189 pKa = 5.61 YY190 pKa = 9.14 KK191 pKa = 10.28 QSIISASVSSSGAAPAPSTHH211 pKa = 7.06 HH212 pKa = 6.47 AQTWWPDD219 pKa = 3.15 WPDD222 pKa = 3.38 AAPDD226 pKa = 3.62 SSTGSTGQRR235 pKa = 11.84 SSSSEE240 pKa = 3.76 SRR242 pKa = 11.84 EE243 pKa = 3.74 GSEE246 pKa = 4.92 DD247 pKa = 2.75 PWEE250 pKa = 4.04 RR251 pKa = 11.84 RR252 pKa = 11.84 EE253 pKa = 3.83 RR254 pKa = 11.84 RR255 pKa = 11.84 LAGTRR260 pKa = 11.84 RR261 pKa = 11.84 GRR263 pKa = 11.84 GGGRR267 pKa = 11.84 GGGRR271 pKa = 11.84 GRR273 pKa = 11.84 GPEE276 pKa = 4.15 ASPSPAPTPSALPVQGDD293 pKa = 4.25 LPQSRR298 pKa = 11.84 QRR300 pKa = 11.84 EE301 pKa = 4.19 EE302 pKa = 4.07 EE303 pKa = 4.14 EE304 pKa = 4.41 EE305 pKa = 4.08 EE306 pKa = 4.56 GGPRR310 pKa = 11.84 PHH312 pKa = 5.97 QACWEE317 pKa = 4.14 NGGEE321 pKa = 4.41 GPSRR325 pKa = 11.84 GSGGIKK331 pKa = 9.97 RR332 pKa = 11.84 RR333 pKa = 11.84 GTGSRR338 pKa = 11.84 GRR340 pKa = 11.84 PPKK343 pKa = 10.5 KK344 pKa = 9.59 KK345 pKa = 8.75 FCRR348 pKa = 11.84 GGRR351 pKa = 11.84 QQGEE355 pKa = 4.33 FHH357 pKa = 6.5 TPSPPTLQSSSFTSPGGDD375 pKa = 3.23 FRR377 pKa = 11.84 GGPHH381 pKa = 7.41 PPRR384 pKa = 11.84 LSLTLLDD391 pKa = 4.11 GKK393 pKa = 11.12 VGGRR397 pKa = 11.84 SGQFGGHH404 pKa = 6.92 PPQGPGQAPAQAGGFPVIVLKK425 pKa = 11.25 GPGNSLKK432 pKa = 10.45 CFRR435 pKa = 11.84 LRR437 pKa = 11.84 CRR439 pKa = 11.84 RR440 pKa = 11.84 GHH442 pKa = 4.98 SQKK445 pKa = 10.23 FLCISTGYY453 pKa = 8.19 TWVCGEE459 pKa = 3.8 GDD461 pKa = 3.18 KK462 pKa = 11.15 VGRR465 pKa = 11.84 QRR467 pKa = 11.84 LMIGFRR473 pKa = 11.84 DD474 pKa = 3.23 EE475 pKa = 4.24 GQRR478 pKa = 11.84 SFFLKK483 pKa = 10.2 NVKK486 pKa = 9.79 IPSTFDD492 pKa = 3.09 LSFGMFDD499 pKa = 3.64 SLL501 pKa = 5.05
Molecular weight: 55.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.572
IPC2_protein 8.419
IPC_protein 8.346
Toseland 8.873
ProMoST 8.858
Dawson 9.253
Bjellqvist 9.194
Wikipedia 9.385
Rodwell 9.385
Grimsley 9.209
Solomon 9.326
Lehninger 9.297
Nozaki 9.341
DTASelect 9.063
Thurlkill 9.165
EMBOSS 9.399
Sillero 9.355
Patrickios 4.952
IPC_peptide 9.326
IPC2_peptide 7.966
IPC2.peptide.svr19 7.704
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2639
96
604
377.0
41.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.267 ± 0.412
2.387 ± 0.709
6.631 ± 0.487
6.328 ± 0.649
4.888 ± 0.257
8.867 ± 1.121
2.728 ± 0.263
2.804 ± 0.287
4.888 ± 0.695
8.147 ± 0.595
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.743 ± 0.294
3.297 ± 0.65
7.768 ± 1.3
3.789 ± 0.486
6.859 ± 0.732
8.45 ± 0.66
5.532 ± 0.668
6.063 ± 0.81
1.402 ± 0.305
2.16 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here