Streptococcus suis (strain 05ZYH33)
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2178 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4VSI4|A4VSI4_STRSY Uncharacterized protein OS=Streptococcus suis (strain 05ZYH33) OX=391295 GN=SSU05_0103 PE=4 SV=1
MM1 pKa = 7.38 AHH3 pKa = 6.23 HH4 pKa = 7.34 HH5 pKa = 5.96 DD6 pKa = 4.33 HH7 pKa = 6.42 EE8 pKa = 6.33 HH9 pKa = 6.69 DD10 pKa = 3.97 HH11 pKa = 6.13 NHH13 pKa = 7.13 DD14 pKa = 3.56 EE15 pKa = 4.62 RR16 pKa = 11.84 EE17 pKa = 4.28 LITLVDD23 pKa = 3.81 DD24 pKa = 4.31 QGNEE28 pKa = 3.98 TLFEE32 pKa = 4.24 ILLTIDD38 pKa = 3.35 GQEE41 pKa = 4.05 EE42 pKa = 4.07 FGKK45 pKa = 10.49 NYY47 pKa = 10.43 VLLIPASAEE56 pKa = 3.59 EE57 pKa = 4.39 DD58 pKa = 3.51 EE59 pKa = 4.52 NGEE62 pKa = 4.24 VEE64 pKa = 4.13 IQAYY68 pKa = 10.18 SYY70 pKa = 11.02 IEE72 pKa = 4.14 NEE74 pKa = 4.13 NGTEE78 pKa = 4.31 GDD80 pKa = 3.87 LQPIPEE86 pKa = 4.84 DD87 pKa = 3.78 ATAEE91 pKa = 3.53 WDD93 pKa = 3.61 MIEE96 pKa = 4.2 EE97 pKa = 4.34 VFNSFMEE104 pKa = 4.43 EE105 pKa = 3.86 EE106 pKa = 4.22
Molecular weight: 12.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.872
IPC_protein 3.808
Toseland 3.643
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.961
Wikipedia 3.643
Rodwell 3.643
Grimsley 3.554
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 3.999
Thurlkill 3.668
EMBOSS 3.656
Sillero 3.923
Patrickios 1.837
IPC_peptide 3.757
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A4VU46|A4VU46_STRSY Arsenate reductase and related proteins glutaredoxin family OS=Streptococcus suis (strain 05ZYH33) OX=391295 GN=SSU05_0669 PE=3 SV=1
MM1 pKa = 7.36 IRR3 pKa = 11.84 HH4 pKa = 5.96 HH5 pKa = 7.05 NYY7 pKa = 10.51 LKK9 pKa = 10.51 IFTFLLILLFIIFLLSLSIGYY30 pKa = 9.97 SNSSFSDD37 pKa = 4.42 FIDD40 pKa = 3.31 IMSGRR45 pKa = 11.84 ASSSTLLIIGRR56 pKa = 11.84 IRR58 pKa = 11.84 LPRR61 pKa = 11.84 IIASIIGGASLALAGLLLQTLTRR84 pKa = 11.84 NPLADD89 pKa = 3.62 SGILGINAGAGLVVALFVGLSSKK112 pKa = 10.36 ISPVLLSLMPLFAMMGGGLTIFLVYY137 pKa = 9.95 WIARR141 pKa = 11.84 KK142 pKa = 9.51 KK143 pKa = 10.83 LYY145 pKa = 10.37 GIHH148 pKa = 7.15 PARR151 pKa = 11.84 LIITGVGISSMLSGIMVSIISQLDD175 pKa = 3.67 DD176 pKa = 3.89 FKK178 pKa = 11.15 MEE180 pKa = 5.35 YY181 pKa = 9.3 IVQWLSGRR189 pKa = 11.84 VNGGDD194 pKa = 2.96 WKK196 pKa = 9.81 TIAIYY201 pKa = 9.77 TPLLLLVWMATFSRR215 pKa = 11.84 SRR217 pKa = 11.84 SLNIMNLNDD226 pKa = 3.53 QTAMALGLHH235 pKa = 6.31 LQRR238 pKa = 11.84 EE239 pKa = 4.26 RR240 pKa = 11.84 RR241 pKa = 11.84 ITLILATALASLSVILVGNITFVGLIAGHH270 pKa = 6.72 IVRR273 pKa = 11.84 KK274 pKa = 9.03 WLGNDD279 pKa = 2.81 HH280 pKa = 6.65 RR281 pKa = 11.84 VIIPATMIIGSFILLLADD299 pKa = 4.13 TIGRR303 pKa = 11.84 VLLVGTGIPTGIIVSLIGAPYY324 pKa = 9.79 FLWLLKK330 pKa = 10.55 QEE332 pKa = 4.42 GG333 pKa = 3.56
Molecular weight: 36.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.882
IPC_protein 10.716
Toseland 10.643
ProMoST 10.394
Dawson 10.774
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 10.979
Grimsley 10.847
Solomon 10.877
Lehninger 10.847
Nozaki 10.613
DTASelect 10.511
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.701
Patrickios 10.672
IPC_peptide 10.877
IPC2_peptide 9.37
IPC2.peptide.svr19 8.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2178
0
2178
609028
30
2274
279.6
31.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.635 ± 0.061
0.597 ± 0.015
5.571 ± 0.046
7.15 ± 0.055
4.532 ± 0.043
6.601 ± 0.06
1.875 ± 0.023
7.189 ± 0.046
6.366 ± 0.052
10.161 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.541 ± 0.029
4.359 ± 0.04
3.372 ± 0.038
4.244 ± 0.049
4.166 ± 0.041
6.126 ± 0.047
5.789 ± 0.053
7.015 ± 0.05
0.919 ± 0.018
3.793 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here