Sulfolobus virus STSV1

Taxonomy: Viruses; Bicaudaviridae; unclassified Bicaudaviridae

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q684D9|Q684D9_9VIRU Uncharacterized protein OS=Sulfolobus virus STSV1 OX=285013 PE=4 SV=1
MM1 pKa = 7.72GFLTNFLQTTFNAAYY16 pKa = 9.09GILMGAFVANLLVDD30 pKa = 5.83AITAAIPNYY39 pKa = 10.09FSGQASQLLSYY50 pKa = 10.74ISGLSLITFLTGLVHH65 pKa = 6.83NPFFGAVVNGAALLGTMSGISLLSNLSSSTQTYY98 pKa = 8.27AQTVFIVAYY107 pKa = 7.8ITYY110 pKa = 9.56VYY112 pKa = 10.43TNALFLFADD121 pKa = 4.31WVPLVVQGLIVAPVIMAFDD140 pKa = 3.7YY141 pKa = 11.01FLIGVFSVAEE151 pKa = 3.81ILYY154 pKa = 8.82MLMALGYY161 pKa = 9.99LPQGVV166 pKa = 3.44

Molecular weight: 17.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q684E6|Q684E6_9VIRU Polysacc_synt_C domain-containing protein OS=Sulfolobus virus STSV1 OX=285013 PE=4 SV=1
MM1 pKa = 7.79AAPKK5 pKa = 10.12IPNLGTNAIDD15 pKa = 3.71VNTTNTASIRR25 pKa = 11.84EE26 pKa = 4.12INVNSLRR33 pKa = 11.84SLLYY37 pKa = 10.95EE38 pKa = 3.76MMNCVRR44 pKa = 11.84TRR46 pKa = 11.84NGIEE50 pKa = 3.98KK51 pKa = 9.65IKK53 pKa = 9.95EE54 pKa = 3.94SEE56 pKa = 4.29RR57 pKa = 11.84IEE59 pKa = 4.42SASKK63 pKa = 9.85LALYY67 pKa = 9.99AGKK70 pKa = 9.77NAGTKK75 pKa = 9.96KK76 pKa = 9.38RR77 pKa = 11.84TNTKK81 pKa = 8.57NTIAVTNVRR90 pKa = 11.84IVTALNVNLLIRR102 pKa = 11.84NSSAPLALKK111 pKa = 10.38

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

21338

69

2308

288.4

32.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.006 ± 0.334

0.455 ± 0.083

4.682 ± 0.284

6.397 ± 0.499

4.162 ± 0.323

4.101 ± 0.312

1.172 ± 0.154

8.609 ± 0.253

7.48 ± 0.555

10.709 ± 0.37

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.029 ± 0.149

6.149 ± 0.432

4.002 ± 0.316

4.283 ± 0.678

3.829 ± 0.326

6.463 ± 0.455

5.98 ± 0.377

6.627 ± 0.324

0.726 ± 0.119

5.141 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski