Aspergillus niger

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta;

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10373 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A100IN92|A0A100IN92_ASPNG Peptide hydrolase OS=Aspergillus niger OX=5061 GN=ABL_06975 PE=3 SV=1
MM1 pKa = 7.59AFKK4 pKa = 8.73TTAGLALLALAGSVKK19 pKa = 10.35AQSVDD24 pKa = 2.83GSKK27 pKa = 11.15YY28 pKa = 10.14NSPTNGPPASYY39 pKa = 9.71FAAATTLPVAALQSAAAKK57 pKa = 10.14ASSVPSKK64 pKa = 10.16ATYY67 pKa = 9.21PVNTDD72 pKa = 2.91KK73 pKa = 11.31NSPKK77 pKa = 9.72STIHH81 pKa = 6.38SDD83 pKa = 2.72WVKK86 pKa = 10.45FNQGAALSWVADD98 pKa = 3.6MDD100 pKa = 4.13VDD102 pKa = 5.35CDD104 pKa = 4.89GIDD107 pKa = 3.7YY108 pKa = 10.19KK109 pKa = 11.38CKK111 pKa = 10.76GNGDD115 pKa = 4.15GLPEE119 pKa = 4.35TNWGALSAYY128 pKa = 6.81EE129 pKa = 4.22VPWIVIPDD137 pKa = 3.55QFLTANEE144 pKa = 4.25DD145 pKa = 3.85LLPGNNIAAVICNGKK160 pKa = 8.75MYY162 pKa = 10.7YY163 pKa = 10.53GILGDD168 pKa = 4.46SNGDD172 pKa = 3.56DD173 pKa = 3.61PEE175 pKa = 4.56VTGEE179 pKa = 4.26ASWLMARR186 pKa = 11.84TCFPDD191 pKa = 4.84DD192 pKa = 4.5DD193 pKa = 5.37LNGAEE198 pKa = 4.32GHH200 pKa = 6.71AEE202 pKa = 3.79ADD204 pKa = 3.06VTYY207 pKa = 10.51IVFTGDD213 pKa = 3.55DD214 pKa = 3.52AVLPSSALDD223 pKa = 3.29KK224 pKa = 11.37NYY226 pKa = 8.69ITNFSTLRR234 pKa = 11.84SMGDD238 pKa = 3.28KK239 pKa = 10.77LVGALASNLGLSKK252 pKa = 10.62SGSGSGSSSSATVPSQTTLATSAASTPSPSGSTATCSWEE291 pKa = 4.15GHH293 pKa = 6.05CEE295 pKa = 3.66GAICSTEE302 pKa = 4.19DD303 pKa = 3.48DD304 pKa = 4.32CSDD307 pKa = 5.38DD308 pKa = 4.41LVCDD312 pKa = 3.68SGKK315 pKa = 10.53CSSADD320 pKa = 3.65DD321 pKa = 5.48DD322 pKa = 4.85EE323 pKa = 7.88DD324 pKa = 5.44DD325 pKa = 5.17VDD327 pKa = 6.2DD328 pKa = 5.81EE329 pKa = 6.52GDD331 pKa = 3.81DD332 pKa = 5.91DD333 pKa = 5.64EE334 pKa = 8.14DD335 pKa = 5.62DD336 pKa = 6.3DD337 pKa = 5.4EE338 pKa = 7.43DD339 pKa = 4.4EE340 pKa = 4.37DD341 pKa = 5.29HH342 pKa = 6.88EE343 pKa = 4.39

Molecular weight: 35.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A117DX63|A0A117DX63_ASPNG G-patch domain protein OS=Aspergillus niger OX=5061 GN=ABL_02148 PE=3 SV=1
MM1 pKa = 7.77SSGRR5 pKa = 11.84LQSQCAAAFALAKK18 pKa = 9.26MWAKK22 pKa = 10.5GCWARR27 pKa = 11.84YY28 pKa = 9.17SLLGLAEE35 pKa = 4.11GLRR38 pKa = 11.84AEE40 pKa = 4.37ARR42 pKa = 11.84NHH44 pKa = 5.94RR45 pKa = 11.84FISACRR51 pKa = 11.84MFVLSRR57 pKa = 11.84SPALVLSVSSPLPLLNSLLGCSSIPRR83 pKa = 11.84LLGIVQAVSVASPLTSPLPTPMASFMQSLPVCSASRR119 pKa = 11.84KK120 pKa = 9.13QSTAVPFYY128 pKa = 10.52LHH130 pKa = 6.98KK131 pKa = 10.69ASQSRR136 pKa = 11.84VSSGTRR142 pKa = 11.84SSFSSLCSSARR153 pKa = 11.84LTPSQITSRR162 pKa = 11.84HH163 pKa = 4.74SSQIISQPQVAAQQCRR179 pKa = 11.84TFLVQLKK186 pKa = 9.86RR187 pKa = 11.84QSQTLKK193 pKa = 8.79EE194 pKa = 4.63TPVSQPPPNLQLTNLPYY211 pKa = 10.58FIRR214 pKa = 11.84RR215 pKa = 11.84TASNQLPVYY224 pKa = 10.11LVTKK228 pKa = 10.78AGGTKK233 pKa = 9.38QQTKK237 pKa = 8.73LQKK240 pKa = 9.74TEE242 pKa = 3.83GDD244 pKa = 3.41LDD246 pKa = 3.76ALRR249 pKa = 11.84NDD251 pKa = 4.02LAQYY255 pKa = 10.81LGLEE259 pKa = 4.44SGDD262 pKa = 3.37PRR264 pKa = 11.84APKK267 pKa = 10.7SPDD270 pKa = 3.01VTINRR275 pKa = 11.84LNGHH279 pKa = 7.11IIVKK283 pKa = 9.36GWRR286 pKa = 11.84KK287 pKa = 9.71PEE289 pKa = 3.64ILKK292 pKa = 10.17FLQEE296 pKa = 4.15RR297 pKa = 11.84NFF299 pKa = 3.45

Molecular weight: 32.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10373

0

10373

5647343

66

6185

544.4

60.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.496 ± 0.019

1.251 ± 0.008

5.67 ± 0.016

6.168 ± 0.026

3.726 ± 0.015

6.797 ± 0.022

2.438 ± 0.01

4.975 ± 0.015

4.439 ± 0.022

9.134 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.008

3.608 ± 0.012

6.032 ± 0.023

4.02 ± 0.014

6.027 ± 0.02

8.275 ± 0.023

6.062 ± 0.017

6.264 ± 0.016

1.498 ± 0.009

2.945 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski