Amino acid dipepetide frequency for Aspergillus niger

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.917AlaAla: 8.917 ± 0.068	1.099AlaCys: 1.099 ± 0.015	4.228AlaAsp: 4.228 ± 0.03	5.042AlaGlu: 5.042 ± 0.04	3.162AlaPhe: 3.162 ± 0.026	5.755AlaGly: 5.755 ± 0.042	1.8AlaHis: 1.8 ± 0.02	4.326AlaIle: 4.326 ± 0.029	3.707AlaLys: 3.707 ± 0.027	7.77AlaLeu: 7.77 ± 0.047	1.995AlaMet: 1.995 ± 0.019	2.876AlaAsn: 2.876 ± 0.024	4.655AlaPro: 4.655 ± 0.042	3.349AlaGln: 3.349 ± 0.028	4.837AlaArg: 4.837 ± 0.033	7.198AlaSer: 7.198 ± 0.04	5.298AlaThr: 5.298 ± 0.033	5.475AlaVal: 5.475 ± 0.039	1.187AlaTrp: 1.187 ± 0.015	2.285AlaTyr: 2.285 ± 0.021	0.0AlaXaa: 0.0 ± 0.0
Cys	0.966CysAla: 0.966 ± 0.014	0.237CysCys: 0.237 ± 0.008	0.675CysAsp: 0.675 ± 0.011	0.611CysGlu: 0.611 ± 0.009	0.548CysPhe: 0.548 ± 0.01	0.921CysGly: 0.921 ± 0.013	0.345CysHis: 0.345 ± 0.009	0.731CysIle: 0.731 ± 0.012	0.455CysLys: 0.455 ± 0.01	1.345CysLeu: 1.345 ± 0.017	0.278CysMet: 0.278 ± 0.007	0.434CysAsn: 0.434 ± 0.009	0.651CysPro: 0.651 ± 0.013	0.46CysGln: 0.46 ± 0.009	0.775CysArg: 0.775 ± 0.012	0.936CysSer: 0.936 ± 0.015	0.712CysThr: 0.712 ± 0.013	0.844CysVal: 0.844 ± 0.013	0.214CysTrp: 0.214 ± 0.007	0.37CysTyr: 0.37 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.633AspAla: 4.633 ± 0.033	0.625AspCys: 0.625 ± 0.013	4.051AspAsp: 4.051 ± 0.041	4.336AspGlu: 4.336 ± 0.037	2.137AspPhe: 2.137 ± 0.02	3.967AspGly: 3.967 ± 0.03	1.285AspHis: 1.285 ± 0.016	3.159AspIle: 3.159 ± 0.026	2.201AspLys: 2.201 ± 0.022	5.215AspLeu: 5.215 ± 0.031	1.272AspMet: 1.272 ± 0.015	1.897AspAsn: 1.897 ± 0.02	3.387AspPro: 3.387 ± 0.026	1.908AspGln: 1.908 ± 0.021	3.116AspArg: 3.116 ± 0.03	4.088AspSer: 4.088 ± 0.035	3.06AspThr: 3.06 ± 0.026	3.724AspVal: 3.724 ± 0.029	0.909AspTrp: 0.909 ± 0.014	1.733AspTyr: 1.733 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	5.295GluAla: 5.295 ± 0.041	0.649GluCys: 0.649 ± 0.011	4.12GluAsp: 4.12 ± 0.034	5.614GluGlu: 5.614 ± 0.068	1.939GluPhe: 1.939 ± 0.019	3.838GluGly: 3.838 ± 0.032	1.413GluHis: 1.413 ± 0.017	2.981GluIle: 2.981 ± 0.025	3.486GluLys: 3.486 ± 0.034	5.157GluLeu: 5.157 ± 0.035	1.478GluMet: 1.478 ± 0.017	2.206GluAsn: 2.206 ± 0.024	2.834GluPro: 2.834 ± 0.044	2.536GluGln: 2.536 ± 0.029	3.885GluArg: 3.885 ± 0.037	4.332GluSer: 4.332 ± 0.031	3.541GluThr: 3.541 ± 0.031	3.703GluVal: 3.703 ± 0.029	0.91GluTrp: 0.91 ± 0.014	1.76GluTyr: 1.76 ± 0.017	0.0GluXaa: 0.0 ± 0.0
Phe	3.001PheAla: 3.001 ± 0.029	0.582PheCys: 0.582 ± 0.012	2.275PheAsp: 2.275 ± 0.021	2.083PheGlu: 2.083 ± 0.021	1.635PhePhe: 1.635 ± 0.02	2.817PheGly: 2.817 ± 0.027	0.961PheHis: 0.961 ± 0.012	1.868PheIle: 1.868 ± 0.02	1.349PheLys: 1.349 ± 0.016	3.618PheLeu: 3.618 ± 0.028	0.771PheMet: 0.771 ± 0.012	1.398PheAsn: 1.398 ± 0.016	2.013PhePro: 2.013 ± 0.019	1.41PheGln: 1.41 ± 0.015	2.033PheArg: 2.033 ± 0.02	3.009PheSer: 3.009 ± 0.026	2.213PheThr: 2.213 ± 0.021	2.392PheVal: 2.392 ± 0.021	0.659PheTrp: 0.659 ± 0.012	1.176PheTyr: 1.176 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	5.24GlyAla: 5.24 ± 0.04	0.912GlyCys: 0.912 ± 0.015	3.636GlyAsp: 3.636 ± 0.03	3.648GlyGlu: 3.648 ± 0.031	2.784GlyPhe: 2.784 ± 0.025	5.503GlyGly: 5.503 ± 0.049	1.674GlyHis: 1.674 ± 0.02	3.561GlyIle: 3.561 ± 0.032	3.204GlyLys: 3.204 ± 0.027	6.237GlyLeu: 6.237 ± 0.041	1.603GlyMet: 1.603 ± 0.017	2.443GlyAsn: 2.443 ± 0.022	3.31GlyPro: 3.31 ± 0.031	2.555GlyGln: 2.555 ± 0.026	4.017GlyArg: 4.017 ± 0.031	5.678GlySer: 5.678 ± 0.039	3.945GlyThr: 3.945 ± 0.03	4.584GlyVal: 4.584 ± 0.038	1.194GlyTrp: 1.194 ± 0.017	2.248GlyTyr: 2.248 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	1.895HisAla: 1.895 ± 0.021	0.338HisCys: 0.338 ± 0.009	1.336HisAsp: 1.336 ± 0.017	1.334HisGlu: 1.334 ± 0.018	0.933HisPhe: 0.933 ± 0.014	1.692HisGly: 1.692 ± 0.018	0.921HisHis: 0.921 ± 0.018	1.258HisIle: 1.258 ± 0.017	0.844HisLys: 0.844 ± 0.013	2.386HisLeu: 2.386 ± 0.023	0.506HisMet: 0.506 ± 0.009	0.884HisAsn: 0.884 ± 0.014	1.776HisPro: 1.776 ± 0.02	0.984HisGln: 0.984 ± 0.014	1.552HisArg: 1.552 ± 0.018	1.828HisSer: 1.828 ± 0.017	1.356HisThr: 1.356 ± 0.015	1.438HisVal: 1.438 ± 0.016	0.365HisTrp: 0.365 ± 0.008	0.755HisTyr: 0.755 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	4.198IleAla: 4.198 ± 0.032	0.804IleCys: 0.804 ± 0.014	2.844IleAsp: 2.844 ± 0.021	2.809IleGlu: 2.809 ± 0.025	2.053IlePhe: 2.053 ± 0.023	3.223IleGly: 3.223 ± 0.028	1.285IleHis: 1.285 ± 0.019	2.627IleIle: 2.627 ± 0.029	1.993IleLys: 1.993 ± 0.02	4.762IleLeu: 4.762 ± 0.038	1.047IleMet: 1.047 ± 0.014	1.811IleAsn: 1.811 ± 0.016	3.201IlePro: 3.201 ± 0.025	1.929IleGln: 1.929 ± 0.019	2.829IleArg: 2.829 ± 0.022	3.874IleSer: 3.874 ± 0.028	2.926IleThr: 2.926 ± 0.025	3.246IleVal: 3.246 ± 0.028	0.749IleTrp: 0.749 ± 0.012	1.534IleTyr: 1.534 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	3.85LysAla: 3.85 ± 0.032	0.465LysCys: 0.465 ± 0.01	2.561LysAsp: 2.561 ± 0.023	3.21LysGlu: 3.21 ± 0.031	1.314LysPhe: 1.314 ± 0.016	2.789LysGly: 2.789 ± 0.022	1.059LysHis: 1.059 ± 0.015	2.086LysIle: 2.086 ± 0.021	2.868LysLys: 2.868 ± 0.05	3.84LysLeu: 3.84 ± 0.029	0.921LysMet: 0.921 ± 0.014	1.576LysAsn: 1.576 ± 0.019	2.533LysPro: 2.533 ± 0.026	1.759LysGln: 1.759 ± 0.022	3.138LysArg: 3.138 ± 0.032	3.22LysSer: 3.22 ± 0.027	2.539LysThr: 2.539 ± 0.025	2.687LysVal: 2.687 ± 0.026	0.634LysTrp: 0.634 ± 0.012	1.341LysTyr: 1.341 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	7.903LeuAla: 7.903 ± 0.043	1.248LeuCys: 1.248 ± 0.018	5.296LeuAsp: 5.296 ± 0.034	5.558LeuGlu: 5.558 ± 0.036	3.458LeuPhe: 3.458 ± 0.029	6.091LeuGly: 6.091 ± 0.039	2.347LeuHis: 2.347 ± 0.02	4.12LeuIle: 4.12 ± 0.033	3.939LeuLys: 3.939 ± 0.032	8.826LeuLeu: 8.826 ± 0.053	1.845LeuMet: 1.845 ± 0.019	3.204LeuAsn: 3.204 ± 0.025	5.604LeuPro: 5.604 ± 0.036	3.997LeuGln: 3.997 ± 0.03	5.862LeuArg: 5.862 ± 0.035	7.588LeuSer: 7.588 ± 0.048	5.051LeuThr: 5.051 ± 0.027	5.579LeuVal: 5.579 ± 0.038	1.247LeuTrp: 1.247 ± 0.019	2.579LeuTyr: 2.579 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.147MetAla: 2.147 ± 0.019	0.244MetCys: 0.244 ± 0.007	1.244MetAsp: 1.244 ± 0.016	1.315MetGlu: 1.315 ± 0.015	0.78MetPhe: 0.78 ± 0.013	1.476MetGly: 1.476 ± 0.021	0.51MetHis: 0.51 ± 0.009	1.023MetIle: 1.023 ± 0.014	0.968MetLys: 0.968 ± 0.012	1.903MetLeu: 1.903 ± 0.019	0.579MetMet: 0.579 ± 0.012	0.807MetAsn: 0.807 ± 0.012	1.23MetPro: 1.23 ± 0.014	0.868MetGln: 0.868 ± 0.014	1.264MetArg: 1.264 ± 0.014	1.895MetSer: 1.895 ± 0.018	1.31MetThr: 1.31 ± 0.016	1.372MetVal: 1.372 ± 0.017	0.27MetTrp: 0.27 ± 0.007	0.556MetTyr: 0.556 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.03AsnAla: 3.03 ± 0.022	0.456AsnCys: 0.456 ± 0.01	1.908AsnAsp: 1.908 ± 0.017	1.993AsnGlu: 1.993 ± 0.021	1.341AsnPhe: 1.341 ± 0.016	2.895AsnGly: 2.895 ± 0.028	0.836AsnHis: 0.836 ± 0.013	2.067AsnIle: 2.067 ± 0.024	1.424AsnLys: 1.424 ± 0.017	3.208AsnLeu: 3.208 ± 0.025	0.842AsnMet: 0.842 ± 0.013	1.498AsnAsn: 1.498 ± 0.019	2.458AsnPro: 2.458 ± 0.024	1.319AsnGln: 1.319 ± 0.016	1.905AsnArg: 1.905 ± 0.018	2.644AsnSer: 2.644 ± 0.022	2.212AsnThr: 2.212 ± 0.023	2.34AsnVal: 2.34 ± 0.022	0.572AsnTrp: 0.572 ± 0.009	1.129AsnTyr: 1.129 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	5.202ProAla: 5.202 ± 0.047	0.531ProCys: 0.531 ± 0.011	3.287ProAsp: 3.287 ± 0.025	3.968ProGlu: 3.968 ± 0.042	2.088ProPhe: 2.088 ± 0.021	3.844ProGly: 3.844 ± 0.032	1.375ProHis: 1.375 ± 0.016	2.578ProIle: 2.578 ± 0.024	2.453ProLys: 2.453 ± 0.022	4.807ProLeu: 4.807 ± 0.03	1.073ProMet: 1.073 ± 0.019	2.171ProAsn: 2.171 ± 0.024	4.804ProPro: 4.804 ± 0.067	2.476ProGln: 2.476 ± 0.031	3.32ProArg: 3.32 ± 0.027	6.124ProSer: 6.124 ± 0.051	4.058ProThr: 4.058 ± 0.033	3.74ProVal: 3.74 ± 0.029	0.795ProTrp: 0.795 ± 0.013	1.628ProTyr: 1.628 ± 0.015	0.0ProXaa: 0.0 ± 0.0
Gln	3.408GlnAla: 3.408 ± 0.032	0.46GlnCys: 0.46 ± 0.009	2.081GlnAsp: 2.081 ± 0.02	2.464GlnGlu: 2.464 ± 0.023	1.311GlnPhe: 1.311 ± 0.015	2.395GlnGly: 2.395 ± 0.023	1.058GlnHis: 1.058 ± 0.015	1.903GlnIle: 1.903 ± 0.019	1.908GlnLys: 1.908 ± 0.024	3.617GlnLeu: 3.617 ± 0.03	0.875GlnMet: 0.875 ± 0.014	1.505GlnAsn: 1.505 ± 0.017	2.585GlnPro: 2.585 ± 0.032	2.419GlnGln: 2.419 ± 0.044	2.603GlnArg: 2.603 ± 0.027	3.205GlnSer: 3.205 ± 0.027	2.359GlnThr: 2.359 ± 0.021	2.224GlnVal: 2.224 ± 0.022	0.596GlnTrp: 0.596 ± 0.012	1.221GlnTyr: 1.221 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	4.617ArgAla: 4.617 ± 0.028	0.721ArgCys: 0.721 ± 0.014	3.349ArgAsp: 3.349 ± 0.03	3.819ArgGlu: 3.819 ± 0.034	2.199ArgPhe: 2.199 ± 0.02	3.644ArgGly: 3.644 ± 0.031	1.537ArgHis: 1.537 ± 0.018	2.876ArgIle: 2.876 ± 0.024	3.229ArgLys: 3.229 ± 0.031	5.645ArgLeu: 5.645 ± 0.036	1.297ArgMet: 1.297 ± 0.016	2.173ArgAsn: 2.173 ± 0.02	3.448ArgPro: 3.448 ± 0.028	2.611ArgGln: 2.611 ± 0.023	4.922ArgArg: 4.922 ± 0.045	4.728ArgSer: 4.728 ± 0.042	3.242ArgThr: 3.242 ± 0.024	3.504ArgVal: 3.504 ± 0.027	0.95ArgTrp: 0.95 ± 0.014	1.763ArgTyr: 1.763 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.697SerAla: 6.697 ± 0.043	0.878SerCys: 0.878 ± 0.014	4.313SerAsp: 4.313 ± 0.032	4.242SerGlu: 4.242 ± 0.033	3.046SerPhe: 3.046 ± 0.025	5.553SerGly: 5.553 ± 0.039	1.984SerHis: 1.984 ± 0.02	4.014SerIle: 4.014 ± 0.029	3.382SerLys: 3.382 ± 0.029	7.454SerLeu: 7.454 ± 0.038	1.721SerMet: 1.721 ± 0.018	2.936SerAsn: 2.936 ± 0.025	5.475SerPro: 5.475 ± 0.057	3.321SerGln: 3.321 ± 0.028	4.916SerArg: 4.916 ± 0.044	8.94SerSer: 8.94 ± 0.072	5.764SerThr: 5.764 ± 0.04	4.767SerVal: 4.767 ± 0.033	1.161SerTrp: 1.161 ± 0.015	2.181SerTyr: 2.181 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	5.251ThrAla: 5.251 ± 0.03	0.755ThrCys: 0.755 ± 0.01	3.017ThrAsp: 3.017 ± 0.026	3.222ThrGlu: 3.222 ± 0.028	2.239ThrPhe: 2.239 ± 0.021	4.286ThrGly: 4.286 ± 0.029	1.347ThrHis: 1.347 ± 0.016	3.174ThrIle: 3.174 ± 0.028	2.414ThrLys: 2.414 ± 0.023	5.453ThrLeu: 5.453 ± 0.03	1.227ThrMet: 1.227 ± 0.016	2.127ThrAsn: 2.127 ± 0.023	4.354ThrPro: 4.354 ± 0.038	2.095ThrGln: 2.095 ± 0.023	3.026ThrArg: 3.026 ± 0.025	5.41ThrSer: 5.41 ± 0.034	4.672ThrThr: 4.672 ± 0.041	3.88ThrVal: 3.88 ± 0.027	0.895ThrTrp: 0.895 ± 0.013	1.776ThrTyr: 1.776 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	5.277ValAla: 5.277 ± 0.037	0.874ValCys: 0.874 ± 0.013	3.839ValAsp: 3.839 ± 0.026	3.921ValGlu: 3.921 ± 0.031	2.495ValPhe: 2.495 ± 0.022	4.115ValGly: 4.115 ± 0.032	1.458ValHis: 1.458 ± 0.018	3.09ValIle: 3.09 ± 0.028	2.72ValLys: 2.72 ± 0.024	5.779ValLeu: 5.779 ± 0.039	1.385ValMet: 1.385 ± 0.017	2.275ValAsn: 2.275 ± 0.022	3.712ValPro: 3.712 ± 0.029	2.469ValGln: 2.469 ± 0.023	3.565ValArg: 3.565 ± 0.027	4.819ValSer: 4.819 ± 0.03	3.645ValThr: 3.645 ± 0.03	4.443ValVal: 4.443 ± 0.037	0.886ValTrp: 0.886 ± 0.012	1.878ValTyr: 1.878 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.147TrpAla: 1.147 ± 0.014	0.194TrpCys: 0.194 ± 0.006	0.928TrpAsp: 0.928 ± 0.015	0.881TrpGlu: 0.881 ± 0.012	0.56TrpPhe: 0.56 ± 0.01	0.968TrpGly: 0.968 ± 0.016	0.365TrpHis: 0.365 ± 0.008	0.789TrpIle: 0.789 ± 0.011	0.794TrpLys: 0.794 ± 0.013	1.418TrpLeu: 1.418 ± 0.021	0.374TrpMet: 0.374 ± 0.009	0.649TrpAsn: 0.649 ± 0.01	0.63TrpPro: 0.63 ± 0.012	0.574TrpGln: 0.574 ± 0.013	0.976TrpArg: 0.976 ± 0.015	1.079TrpSer: 1.079 ± 0.014	0.963TrpThr: 0.963 ± 0.015	0.937TrpVal: 0.937 ± 0.012	0.289TrpTrp: 0.289 ± 0.008	0.461TrpTyr: 0.461 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.291TyrAla: 2.291 ± 0.021	0.435TyrCys: 0.435 ± 0.008	1.714TyrAsp: 1.714 ± 0.017	1.604TyrGlu: 1.604 ± 0.017	1.239TyrPhe: 1.239 ± 0.017	2.202TyrGly: 2.202 ± 0.025	0.824TyrHis: 0.824 ± 0.013	1.515TyrIle: 1.515 ± 0.019	1.054TyrLys: 1.054 ± 0.015	2.902TyrLeu: 2.902 ± 0.023	0.672TyrMet: 0.672 ± 0.01	1.204TyrAsn: 1.204 ± 0.016	1.662TyrPro: 1.662 ± 0.019	1.158TyrGln: 1.158 ± 0.017	1.733TyrArg: 1.733 ± 0.019	2.151TyrSer: 2.151 ± 0.022	1.755TyrThr: 1.755 ± 0.019	1.767TyrVal: 1.767 ± 0.02	0.495TyrTrp: 0.495 ± 0.011	1.07TyrTyr: 1.07 ± 0.035	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10373 proteins (5647343 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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