Staphylococcus lentus
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2457 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A178PEJ9|A0A178PEJ9_STALE Gram_pos_anchor domain-containing protein OS=Staphylococcus lentus OX=42858 GN=AXY37_08430 PE=4 SV=1
MM1 pKa = 7.37 VHH3 pKa = 6.59 EE4 pKa = 5.25 LGTVGMVCPFPLIEE18 pKa = 4.83 AQNKK22 pKa = 8.3 MDD24 pKa = 4.04 EE25 pKa = 4.34 LQAGEE30 pKa = 4.02 QLKK33 pKa = 10.22 IDD35 pKa = 4.4 FDD37 pKa = 4.17 CTQATEE43 pKa = 4.89 ALPNWAADD51 pKa = 3.18 NGYY54 pKa = 9.21 PVTNYY59 pKa = 9.8 EE60 pKa = 4.18 QVGDD64 pKa = 4.22 ASWTITVEE72 pKa = 4.16 KK73 pKa = 10.49 QQ74 pKa = 2.69
Molecular weight: 8.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 4.012
IPC_protein 3.872
Toseland 3.706
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.617
Solomon 3.821
Lehninger 3.783
Nozaki 3.986
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.77
Sillero 3.986
Patrickios 1.888
IPC_peptide 3.821
IPC2_peptide 3.973
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|A0A178NE84|A0A178NE84_STALE ABC transporter ATP-binding protein OS=Staphylococcus lentus OX=42858 GN=AXY37_11420 PE=4 SV=1
MM1 pKa = 7.49 VKK3 pKa = 9.1 RR4 pKa = 11.84 TYY6 pKa = 10.35 QPNKK10 pKa = 8.16 RR11 pKa = 11.84 KK12 pKa = 9.54 HH13 pKa = 5.99 SKK15 pKa = 8.91 VHH17 pKa = 5.68 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSSKK26 pKa = 10.48 NGRR29 pKa = 11.84 KK30 pKa = 8.91 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.05 GRR40 pKa = 11.84 KK41 pKa = 8.7 VLSAA45 pKa = 4.05
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2457
0
2457
745563
37
1497
303.4
34.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.924 ± 0.049
0.625 ± 0.012
5.695 ± 0.045
7.459 ± 0.057
4.566 ± 0.037
6.344 ± 0.046
2.133 ± 0.023
8.997 ± 0.051
7.679 ± 0.053
9.316 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.684 ± 0.022
5.5 ± 0.041
3.213 ± 0.023
3.571 ± 0.035
3.315 ± 0.033
6.098 ± 0.036
5.468 ± 0.027
6.717 ± 0.039
0.793 ± 0.017
3.902 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here