Vibrio phage pTD1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Gorgonvirinae; Tidunavirus; Vibrio virus pTD1

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q2U301|A0A1Q2U301_9CAUD Phage protein OS=Vibrio phage pTD1 OX=1938577 PE=4 SV=1
MM1 pKa = 7.28FVTIHH6 pKa = 6.77HH7 pKa = 7.35EE8 pKa = 4.0DD9 pKa = 3.69TEE11 pKa = 4.42EE12 pKa = 4.84CDD14 pKa = 3.39EE15 pKa = 4.52DD16 pKa = 4.42TFSDD20 pKa = 4.3GADD23 pKa = 3.06GGFYY27 pKa = 10.11RR28 pKa = 11.84ASPADD33 pKa = 3.77PFHH36 pKa = 7.1WDD38 pKa = 2.81

Molecular weight: 4.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q2U2P9|A0A1Q2U2P9_9CAUD Phage protein OS=Vibrio phage pTD1 OX=1938577 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.25KK3 pKa = 10.03KK4 pKa = 10.21KK5 pKa = 8.8QRR7 pKa = 11.84KK8 pKa = 8.55KK9 pKa = 8.81EE10 pKa = 3.91KK11 pKa = 10.06KK12 pKa = 8.03RR13 pKa = 11.84QKK15 pKa = 10.01EE16 pKa = 3.8AALVLAKK23 pKa = 9.13FQPNPGMILTPTQRR37 pKa = 11.84ALHH40 pKa = 6.22LSMVNPTRR48 pKa = 11.84FF49 pKa = 3.07

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

209

0

209

74130

38

2486

354.7

40.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.146 ± 0.164

0.812 ± 0.059

6.501 ± 0.106

7.306 ± 0.15

4.145 ± 0.078

6.486 ± 0.2

2.202 ± 0.095

5.787 ± 0.129

5.887 ± 0.186

8.955 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.853 ± 0.092

5.152 ± 0.1

4.109 ± 0.118

3.379 ± 0.094

4.995 ± 0.154

6.089 ± 0.104

6.912 ± 0.205

7.116 ± 0.207

1.167 ± 0.053

4.001 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski