Amino acid dipepetide frequency for Vibrio phage pTD1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.047AlaAla: 4.047 ± 0.314
0.432AlaCys: 0.432 ± 0.08
3.359AlaAsp: 3.359 ± 0.217
4.317AlaGlu: 4.317 ± 0.272
2.428AlaPhe: 2.428 ± 0.17
4.573AlaGly: 4.573 ± 0.334
1.389AlaHis: 1.389 ± 0.127
3.764AlaIle: 3.764 ± 0.245
4.074AlaLys: 4.074 ± 0.344
5.882AlaLeu: 5.882 ± 0.368
1.848AlaMet: 1.848 ± 0.168
2.968AlaAsn: 2.968 ± 0.211
2.347AlaPro: 2.347 ± 0.214
1.929AlaGln: 1.929 ± 0.17
3.292AlaArg: 3.292 ± 0.216
3.602AlaSer: 3.602 ± 0.257
3.966AlaThr: 3.966 ± 0.305
4.195AlaVal: 4.195 ± 0.289
0.661AlaTrp: 0.661 ± 0.093
2.388AlaTyr: 2.388 ± 0.169
0.0AlaXaa: 0.0 ± 0.0
Cys
0.486CysAla: 0.486 ± 0.078
0.067CysCys: 0.067 ± 0.029
0.594CysAsp: 0.594 ± 0.081
0.553CysGlu: 0.553 ± 0.102
0.405CysPhe: 0.405 ± 0.066
0.594CysGly: 0.594 ± 0.09
0.189CysHis: 0.189 ± 0.049
0.432CysIle: 0.432 ± 0.082
0.472CysLys: 0.472 ± 0.087
0.877CysLeu: 0.877 ± 0.12
0.189CysMet: 0.189 ± 0.051
0.31CysAsn: 0.31 ± 0.065
0.432CysPro: 0.432 ± 0.092
0.229CysGln: 0.229 ± 0.064
0.472CysArg: 0.472 ± 0.084
0.432CysSer: 0.432 ± 0.076
0.364CysThr: 0.364 ± 0.078
0.58CysVal: 0.58 ± 0.103
0.135CysTrp: 0.135 ± 0.039
0.31CysTyr: 0.31 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
3.642AspAla: 3.642 ± 0.228
0.445AspCys: 0.445 ± 0.075
3.939AspAsp: 3.939 ± 0.241
4.897AspGlu: 4.897 ± 0.257
3.13AspPhe: 3.13 ± 0.195
5.207AspGly: 5.207 ± 0.467
1.484AspHis: 1.484 ± 0.134
3.966AspIle: 3.966 ± 0.233
3.305AspLys: 3.305 ± 0.249
6.516AspLeu: 6.516 ± 0.319
1.7AspMet: 1.7 ± 0.18
2.738AspAsn: 2.738 ± 0.209
2.833AspPro: 2.833 ± 0.195
2.685AspGln: 2.685 ± 0.225
3.332AspArg: 3.332 ± 0.231
3.062AspSer: 3.062 ± 0.178
3.723AspThr: 3.723 ± 0.25
4.748AspVal: 4.748 ± 0.236
0.877AspTrp: 0.877 ± 0.089
2.779AspTyr: 2.779 ± 0.185
0.0AspXaa: 0.0 ± 0.0
Glu
4.627GluAla: 4.627 ± 0.308
0.85GluCys: 0.85 ± 0.12
3.912GluAsp: 3.912 ± 0.219
5.72GluGlu: 5.72 ± 0.492
3.346GluPhe: 3.346 ± 0.166
4.344GluGly: 4.344 ± 0.316
1.875GluHis: 1.875 ± 0.152
4.384GluIle: 4.384 ± 0.218
3.197GluLys: 3.197 ± 0.236
8.027GluLeu: 8.027 ± 0.348
2.334GluMet: 2.334 ± 0.165
2.819GluAsn: 2.819 ± 0.169
2.455GluPro: 2.455 ± 0.23
3.076GluGln: 3.076 ± 0.214
3.399GluArg: 3.399 ± 0.247
4.762GluSer: 4.762 ± 0.279
4.641GluThr: 4.641 ± 0.262
5.329GluVal: 5.329 ± 0.276
0.958GluTrp: 0.958 ± 0.117
3.008GluTyr: 3.008 ± 0.176
0.0GluXaa: 0.0 ± 0.0
Phe
2.32PheAla: 2.32 ± 0.178
0.337PheCys: 0.337 ± 0.058
3.467PheAsp: 3.467 ± 0.209
2.846PheGlu: 2.846 ± 0.224
1.511PhePhe: 1.511 ± 0.137
2.765PheGly: 2.765 ± 0.247
0.701PheHis: 0.701 ± 0.101
2.334PheIle: 2.334 ± 0.2
2.819PheLys: 2.819 ± 0.224
3.022PheLeu: 3.022 ± 0.196
1.201PheMet: 1.201 ± 0.129
2.765PheAsn: 2.765 ± 0.175
1.336PhePro: 1.336 ± 0.132
1.174PheGln: 1.174 ± 0.123
2.293PheArg: 2.293 ± 0.171
2.711PheSer: 2.711 ± 0.207
3.332PheThr: 3.332 ± 0.252
2.266PheVal: 2.266 ± 0.218
0.364PheTrp: 0.364 ± 0.064
1.889PheTyr: 1.889 ± 0.172
0.0PheXaa: 0.0 ± 0.0
Gly
3.319GlyAla: 3.319 ± 0.249
0.54GlyCys: 0.54 ± 0.096
4.357GlyAsp: 4.357 ± 0.321
5.288GlyGlu: 5.288 ± 0.385
2.32GlyPhe: 2.32 ± 0.155
4.114GlyGly: 4.114 ± 0.34
1.066GlyHis: 1.066 ± 0.127
3.696GlyIle: 3.696 ± 0.24
5.018GlyLys: 5.018 ± 0.284
5.625GlyLeu: 5.625 ± 0.252
2.199GlyMet: 2.199 ± 0.182
3.588GlyAsn: 3.588 ± 0.264
1.524GlyPro: 1.524 ± 0.135
1.956GlyGln: 1.956 ± 0.22
3.265GlyArg: 3.265 ± 0.202
3.372GlySer: 3.372 ± 0.291
4.843GlyThr: 4.843 ± 0.383
5.099GlyVal: 5.099 ± 0.329
0.904GlyTrp: 0.904 ± 0.113
2.765GlyTyr: 2.765 ± 0.181
0.0GlyXaa: 0.0 ± 0.0
His
1.443HisAla: 1.443 ± 0.154
0.121HisCys: 0.121 ± 0.039
1.201HisAsp: 1.201 ± 0.125
1.322HisGlu: 1.322 ± 0.151
1.012HisPhe: 1.012 ± 0.123
1.268HisGly: 1.268 ± 0.129
0.715HisHis: 0.715 ± 0.097
1.268HisIle: 1.268 ± 0.137
0.836HisLys: 0.836 ± 0.129
2.199HisLeu: 2.199 ± 0.18
0.499HisMet: 0.499 ± 0.11
1.12HisAsn: 1.12 ± 0.151
1.43HisPro: 1.43 ± 0.152
0.971HisGln: 0.971 ± 0.132
1.255HisArg: 1.255 ± 0.136
0.971HisSer: 0.971 ± 0.123
1.484HisThr: 1.484 ± 0.136
1.484HisVal: 1.484 ± 0.171
0.351HisTrp: 0.351 ± 0.077
1.066HisTyr: 1.066 ± 0.115
0.0HisXaa: 0.0 ± 0.0
Ile
4.034IleAla: 4.034 ± 0.237
0.432IleCys: 0.432 ± 0.066
4.087IleAsp: 4.087 ± 0.226
4.007IleGlu: 4.007 ± 0.254
1.713IlePhe: 1.713 ± 0.154
3.143IleGly: 3.143 ± 0.219
1.43IleHis: 1.43 ± 0.147
2.846IleIle: 2.846 ± 0.226
4.438IleLys: 4.438 ± 0.257
4.007IleLeu: 4.007 ± 0.262
1.619IleMet: 1.619 ± 0.15
3.656IleAsn: 3.656 ± 0.249
3.035IlePro: 3.035 ± 0.228
2.077IleGln: 2.077 ± 0.159
3.211IleArg: 3.211 ± 0.211
3.453IleSer: 3.453 ± 0.188
4.249IleThr: 4.249 ± 0.23
3.818IleVal: 3.818 ± 0.249
0.526IleTrp: 0.526 ± 0.085
2.091IleTyr: 2.091 ± 0.176
0.0IleXaa: 0.0 ± 0.0
Lys
4.735LysAla: 4.735 ± 0.317
0.405LysCys: 0.405 ± 0.075
4.492LysAsp: 4.492 ± 0.251
5.45LysGlu: 5.45 ± 0.377
2.253LysPhe: 2.253 ± 0.161
3.332LysGly: 3.332 ± 0.255
1.551LysHis: 1.551 ± 0.154
2.995LysIle: 2.995 ± 0.257
2.482LysLys: 2.482 ± 0.24
5.356LysLeu: 5.356 ± 0.31
1.457LysMet: 1.457 ± 0.159
2.374LysAsn: 2.374 ± 0.191
2.577LysPro: 2.577 ± 0.188
2.104LysGln: 2.104 ± 0.18
3.103LysArg: 3.103 ± 0.237
3.44LysSer: 3.44 ± 0.245
3.48LysThr: 3.48 ± 0.21
4.56LysVal: 4.56 ± 0.28
0.701LysTrp: 0.701 ± 0.109
2.023LysTyr: 2.023 ± 0.163
0.0LysXaa: 0.0 ± 0.0
Leu
5.14LeuAla: 5.14 ± 0.299
0.742LeuCys: 0.742 ± 0.095
6.219LeuAsp: 6.219 ± 0.284
6.543LeuGlu: 6.543 ± 0.3
3.534LeuPhe: 3.534 ± 0.235
5.949LeuGly: 5.949 ± 0.276
1.7LeuHis: 1.7 ± 0.188
4.708LeuIle: 4.708 ± 0.259
5.72LeuLys: 5.72 ± 0.341
6.758LeuLeu: 6.758 ± 0.38
2.563LeuMet: 2.563 ± 0.181
5.625LeuAsn: 5.625 ± 0.273
4.074LeuPro: 4.074 ± 0.251
2.428LeuGln: 2.428 ± 0.212
4.398LeuArg: 4.398 ± 0.259
6.502LeuSer: 6.502 ± 0.297
7.5LeuThr: 7.5 ± 0.378
5.625LeuVal: 5.625 ± 0.296
0.688LeuTrp: 0.688 ± 0.107
3.13LeuTyr: 3.13 ± 0.221
0.0LeuXaa: 0.0 ± 0.0
Met
2.064MetAla: 2.064 ± 0.179
0.256MetCys: 0.256 ± 0.07
1.889MetAsp: 1.889 ± 0.18
2.347MetGlu: 2.347 ± 0.188
1.079MetPhe: 1.079 ± 0.119
1.592MetGly: 1.592 ± 0.154
0.445MetHis: 0.445 ± 0.078
1.835MetIle: 1.835 ± 0.153
1.713MetLys: 1.713 ± 0.151
2.01MetLeu: 2.01 ± 0.168
0.715MetMet: 0.715 ± 0.11
1.619MetAsn: 1.619 ± 0.164
0.863MetPro: 0.863 ± 0.107
0.715MetGln: 0.715 ± 0.095
1.241MetArg: 1.241 ± 0.132
2.563MetSer: 2.563 ± 0.219
2.401MetThr: 2.401 ± 0.196
2.064MetVal: 2.064 ± 0.169
0.297MetTrp: 0.297 ± 0.059
0.823MetTyr: 0.823 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
3.224AsnAla: 3.224 ± 0.182
0.31AsnCys: 0.31 ± 0.072
2.968AsnAsp: 2.968 ± 0.209
3.467AsnGlu: 3.467 ± 0.196
1.889AsnPhe: 1.889 ± 0.186
4.249AsnGly: 4.249 ± 0.254
1.309AsnHis: 1.309 ± 0.153
3.062AsnIle: 3.062 ± 0.191
2.779AsnLys: 2.779 ± 0.191
4.411AsnLeu: 4.411 ± 0.303
1.416AsnMet: 1.416 ± 0.132
2.644AsnAsn: 2.644 ± 0.238
2.968AsnPro: 2.968 ± 0.195
2.023AsnGln: 2.023 ± 0.188
2.927AsnArg: 2.927 ± 0.22
2.9AsnSer: 2.9 ± 0.202
2.981AsnThr: 2.981 ± 0.168
3.426AsnVal: 3.426 ± 0.205
0.634AsnTrp: 0.634 ± 0.1
1.929AsnTyr: 1.929 ± 0.171
0.0AsnXaa: 0.0 ± 0.0
Pro
2.442ProAla: 2.442 ± 0.185
0.297ProCys: 0.297 ± 0.049
2.725ProAsp: 2.725 ± 0.178
3.534ProGlu: 3.534 ± 0.235
1.97ProPhe: 1.97 ± 0.156
2.145ProGly: 2.145 ± 0.234
0.823ProHis: 0.823 ± 0.115
2.253ProIle: 2.253 ± 0.189
2.779ProLys: 2.779 ± 0.196
3.103ProLeu: 3.103 ± 0.219
1.16ProMet: 1.16 ± 0.099
2.347ProAsn: 2.347 ± 0.179
1.147ProPro: 1.147 ± 0.12
1.12ProGln: 1.12 ± 0.114
1.497ProArg: 1.497 ± 0.134
2.563ProSer: 2.563 ± 0.208
3.399ProThr: 3.399 ± 0.231
3.548ProVal: 3.548 ± 0.348
0.472ProTrp: 0.472 ± 0.077
1.767ProTyr: 1.767 ± 0.186
0.0ProXaa: 0.0 ± 0.0
Gln
2.05GlnAla: 2.05 ± 0.171
0.378GlnCys: 0.378 ± 0.071
1.551GlnAsp: 1.551 ± 0.15
2.118GlnGlu: 2.118 ± 0.174
1.592GlnPhe: 1.592 ± 0.147
1.794GlnGly: 1.794 ± 0.166
1.066GlnHis: 1.066 ± 0.125
2.374GlnIle: 2.374 ± 0.173
1.362GlnLys: 1.362 ± 0.187
3.831GlnLeu: 3.831 ± 0.249
0.998GlnMet: 0.998 ± 0.122
1.336GlnAsn: 1.336 ± 0.142
1.389GlnPro: 1.389 ± 0.117
1.457GlnGln: 1.457 ± 0.186
1.983GlnArg: 1.983 ± 0.164
1.97GlnSer: 1.97 ± 0.166
2.307GlnThr: 2.307 ± 0.174
2.239GlnVal: 2.239 ± 0.148
0.472GlnTrp: 0.472 ± 0.085
1.524GlnTyr: 1.524 ± 0.154
0.0GlnXaa: 0.0 ± 0.0
Arg
3.521ArgAla: 3.521 ± 0.228
0.405ArgCys: 0.405 ± 0.084
3.372ArgAsp: 3.372 ± 0.216
3.076ArgGlu: 3.076 ± 0.224
2.28ArgPhe: 2.28 ± 0.197
2.738ArgGly: 2.738 ± 0.213
1.025ArgHis: 1.025 ± 0.11
3.062ArgIle: 3.062 ± 0.212
2.941ArgLys: 2.941 ± 0.213
5.126ArgLeu: 5.126 ± 0.255
1.592ArgMet: 1.592 ± 0.174
2.631ArgAsn: 2.631 ± 0.189
1.835ArgPro: 1.835 ± 0.154
1.983ArgGln: 1.983 ± 0.164
2.455ArgArg: 2.455 ± 0.214
2.671ArgSer: 2.671 ± 0.197
3.399ArgThr: 3.399 ± 0.218
3.116ArgVal: 3.116 ± 0.21
0.621ArgTrp: 0.621 ± 0.094
2.104ArgTyr: 2.104 ± 0.182
0.0ArgXaa: 0.0 ± 0.0
Ser
3.75SerAla: 3.75 ± 0.266
0.567SerCys: 0.567 ± 0.103
4.06SerAsp: 4.06 ± 0.269
4.128SerGlu: 4.128 ± 0.231
3.184SerPhe: 3.184 ± 0.216
4.195SerGly: 4.195 ± 0.224
1.106SerHis: 1.106 ± 0.12
4.249SerIle: 4.249 ± 0.242
3.791SerLys: 3.791 ± 0.215
5.666SerLeu: 5.666 ± 0.318
1.646SerMet: 1.646 ± 0.157
3.062SerAsn: 3.062 ± 0.222
2.212SerPro: 2.212 ± 0.17
1.848SerGln: 1.848 ± 0.18
2.644SerArg: 2.644 ± 0.209
3.737SerSer: 3.737 ± 0.242
3.575SerThr: 3.575 ± 0.233
4.627SerVal: 4.627 ± 0.24
0.607SerTrp: 0.607 ± 0.087
2.239SerTyr: 2.239 ± 0.169
0.0SerXaa: 0.0 ± 0.0
Thr
3.993ThrAla: 3.993 ± 0.312
0.351ThrCys: 0.351 ± 0.064
4.519ThrAsp: 4.519 ± 0.276
4.641ThrGlu: 4.641 ± 0.269
2.995ThrPhe: 2.995 ± 0.218
4.519ThrGly: 4.519 ± 0.266
1.349ThrHis: 1.349 ± 0.145
4.303ThrIle: 4.303 ± 0.297
3.953ThrLys: 3.953 ± 0.214
6.799ThrLeu: 6.799 ± 0.324
1.875ThrMet: 1.875 ± 0.164
3.346ThrAsn: 3.346 ± 0.225
3.534ThrPro: 3.534 ± 0.211
2.145ThrGln: 2.145 ± 0.179
2.819ThrArg: 2.819 ± 0.207
4.06ThrSer: 4.06 ± 0.304
4.6ThrThr: 4.6 ± 0.276
5.504ThrVal: 5.504 ± 0.368
0.985ThrTrp: 0.985 ± 0.168
2.833ThrTyr: 2.833 ± 0.195
0.0ThrXaa: 0.0 ± 0.0
Val
3.912ValAla: 3.912 ± 0.25
0.648ValCys: 0.648 ± 0.096
4.951ValAsp: 4.951 ± 0.252
5.409ValGlu: 5.409 ± 0.324
2.415ValPhe: 2.415 ± 0.17
4.964ValGly: 4.964 ± 0.299
1.497ValHis: 1.497 ± 0.165
3.683ValIle: 3.683 ± 0.207
4.951ValLys: 4.951 ± 0.275
5.329ValLeu: 5.329 ± 0.315
2.104ValMet: 2.104 ± 0.151
3.98ValAsn: 3.98 ± 0.211
2.995ValPro: 2.995 ± 0.237
1.902ValGln: 1.902 ± 0.148
3.13ValArg: 3.13 ± 0.221
4.587ValSer: 4.587 ± 0.264
5.49ValThr: 5.49 ± 0.351
5.18ValVal: 5.18 ± 0.375
0.863ValTrp: 0.863 ± 0.099
3.157ValTyr: 3.157 ± 0.257
0.0ValXaa: 0.0 ± 0.0
Trp
0.486TrpAla: 0.486 ± 0.091
0.229TrpCys: 0.229 ± 0.057
0.796TrpAsp: 0.796 ± 0.095
0.823TrpGlu: 0.823 ± 0.091
0.715TrpPhe: 0.715 ± 0.113
0.594TrpGly: 0.594 ± 0.086
0.216TrpHis: 0.216 ± 0.057
0.513TrpIle: 0.513 ± 0.076
0.567TrpLys: 0.567 ± 0.1
1.43TrpLeu: 1.43 ± 0.14
0.513TrpMet: 0.513 ± 0.088
0.54TrpAsn: 0.54 ± 0.084
0.135TrpPro: 0.135 ± 0.047
0.283TrpGln: 0.283 ± 0.066
0.459TrpArg: 0.459 ± 0.081
1.039TrpSer: 1.039 ± 0.138
0.688TrpThr: 0.688 ± 0.123
1.039TrpVal: 1.039 ± 0.147
0.094TrpTrp: 0.094 ± 0.034
0.513TrpTyr: 0.513 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.226TyrAla: 2.226 ± 0.163
0.31TyrCys: 0.31 ± 0.067
2.55TyrAsp: 2.55 ± 0.199
2.577TyrGlu: 2.577 ± 0.203
1.686TyrPhe: 1.686 ± 0.152
2.779TyrGly: 2.779 ± 0.22
1.079TyrHis: 1.079 ± 0.13
2.145TyrIle: 2.145 ± 0.161
1.673TyrLys: 1.673 ± 0.143
3.575TyrLeu: 3.575 ± 0.274
0.89TyrMet: 0.89 ± 0.104
2.05TyrAsn: 2.05 ± 0.182
1.97TyrPro: 1.97 ± 0.18
1.686TyrGln: 1.686 ± 0.109
2.779TyrArg: 2.779 ± 0.186
2.496TyrSer: 2.496 ± 0.211
2.698TyrThr: 2.698 ± 0.224
2.711TyrVal: 2.711 ± 0.2
0.459TyrTrp: 0.459 ± 0.081
1.673TyrTyr: 1.673 ± 0.158
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 209 proteins (74130 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski