Amino acid dipepetide frequency for Paenibacillaceae bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.773AlaAla: 9.773 ± 0.117	0.739AlaCys: 0.739 ± 0.019	4.666AlaAsp: 4.666 ± 0.135	5.67AlaGlu: 5.67 ± 0.077	3.329AlaPhe: 3.329 ± 0.04	6.774AlaGly: 6.774 ± 0.066	1.505AlaHis: 1.505 ± 0.027	5.607AlaIle: 5.607 ± 0.052	4.054AlaLys: 4.054 ± 0.052	8.582AlaLeu: 8.582 ± 0.076	2.285AlaMet: 2.285 ± 0.035	2.897AlaAsn: 2.897 ± 0.04	2.726AlaPro: 2.726 ± 0.04	2.962AlaGln: 2.962 ± 0.045	3.771AlaArg: 3.771 ± 0.048	5.31AlaSer: 5.31 ± 0.058	3.647AlaThr: 3.647 ± 0.063	6.637AlaVal: 6.637 ± 0.059	0.982AlaTrp: 0.982 ± 0.021	2.691AlaTyr: 2.691 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	0.569CysAla: 0.569 ± 0.021	0.131CysCys: 0.131 ± 0.01	0.367CysAsp: 0.367 ± 0.015	0.438CysGlu: 0.438 ± 0.015	0.325CysPhe: 0.325 ± 0.014	0.745CysGly: 0.745 ± 0.021	0.192CysHis: 0.192 ± 0.01	0.486CysIle: 0.486 ± 0.017	0.335CysLys: 0.335 ± 0.013	0.735CysLeu: 0.735 ± 0.018	0.203CysMet: 0.203 ± 0.009	0.269CysAsn: 0.269 ± 0.011	0.332CysPro: 0.332 ± 0.013	0.223CysGln: 0.223 ± 0.01	0.44CysArg: 0.44 ± 0.015	0.61CysSer: 0.61 ± 0.019	0.415CysThr: 0.415 ± 0.014	0.447CysVal: 0.447 ± 0.014	0.107CysTrp: 0.107 ± 0.007	0.281CysTyr: 0.281 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	4.121AspAla: 4.121 ± 0.126	0.392AspCys: 0.392 ± 0.014	2.606AspAsp: 2.606 ± 0.038	3.967AspGlu: 3.967 ± 0.048	2.129AspPhe: 2.129 ± 0.033	4.211AspGly: 4.211 ± 0.059	1.134AspHis: 1.134 ± 0.027	3.483AspIle: 3.483 ± 0.041	2.794AspLys: 2.794 ± 0.04	4.685AspLeu: 4.685 ± 0.049	1.265AspMet: 1.265 ± 0.024	2.186AspAsn: 2.186 ± 0.032	2.233AspPro: 2.233 ± 0.033	1.957AspGln: 1.957 ± 0.032	2.691AspArg: 2.691 ± 0.037	2.844AspSer: 2.844 ± 0.041	2.458AspThr: 2.458 ± 0.03	3.431AspVal: 3.431 ± 0.038	0.884AspTrp: 0.884 ± 0.018	2.238AspTyr: 2.238 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	6.075GluAla: 6.075 ± 0.079	0.351GluCys: 0.351 ± 0.014	3.089GluAsp: 3.089 ± 0.045	5.164GluGlu: 5.164 ± 0.065	2.186GluPhe: 2.186 ± 0.036	4.397GluGly: 4.397 ± 0.047	1.548GluHis: 1.548 ± 0.029	4.309GluIle: 4.309 ± 0.054	3.622GluLys: 3.622 ± 0.045	7.423GluLeu: 7.423 ± 0.086	1.995GluMet: 1.995 ± 0.036	2.433GluAsn: 2.433 ± 0.036	2.315GluPro: 2.315 ± 0.034	4.319GluGln: 4.319 ± 0.054	4.205GluArg: 4.205 ± 0.057	3.377GluSer: 3.377 ± 0.04	3.371GluThr: 3.371 ± 0.037	4.325GluVal: 4.325 ± 0.048	0.983GluTrp: 0.983 ± 0.022	2.008GluTyr: 2.008 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.292PheAla: 3.292 ± 0.037	0.331PheCys: 0.331 ± 0.013	2.321PheAsp: 2.321 ± 0.036	2.497PheGlu: 2.497 ± 0.04	1.826PhePhe: 1.826 ± 0.037	3.099PheGly: 3.099 ± 0.041	0.979PheHis: 0.979 ± 0.023	2.967PheIle: 2.967 ± 0.041	2.109PheLys: 2.109 ± 0.034	3.78PheLeu: 3.78 ± 0.051	1.081PheMet: 1.081 ± 0.023	1.808PheAsn: 1.808 ± 0.036	1.6PhePro: 1.6 ± 0.027	1.503PheGln: 1.503 ± 0.025	2.047PheArg: 2.047 ± 0.034	2.832PheSer: 2.832 ± 0.039	2.394PheThr: 2.394 ± 0.036	2.923PheVal: 2.923 ± 0.039	0.553PheTrp: 0.553 ± 0.017	1.555PheTyr: 1.555 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	5.579GlyAla: 5.579 ± 0.088	0.704GlyCys: 0.704 ± 0.017	3.432GlyAsp: 3.432 ± 0.041	4.742GlyGlu: 4.742 ± 0.057	3.124GlyPhe: 3.124 ± 0.041	5.37GlyGly: 5.37 ± 0.066	1.394GlyHis: 1.394 ± 0.027	5.33GlyIle: 5.33 ± 0.054	4.381GlyLys: 4.381 ± 0.053	7.078GlyLeu: 7.078 ± 0.06	2.3GlyMet: 2.3 ± 0.033	2.913GlyAsn: 2.913 ± 0.051	1.799GlyPro: 1.799 ± 0.036	2.873GlyGln: 2.873 ± 0.044	3.5GlyArg: 3.5 ± 0.048	4.797GlySer: 4.797 ± 0.045	4.223GlyThr: 4.223 ± 0.051	4.851GlyVal: 4.851 ± 0.052	1.103GlyTrp: 1.103 ± 0.026	2.907GlyTyr: 2.907 ± 0.043	0.0GlyXaa: 0.0 ± 0.001
His	1.647HisAla: 1.647 ± 0.031	0.224HisCys: 0.224 ± 0.01	1.059HisAsp: 1.059 ± 0.024	1.3HisGlu: 1.3 ± 0.026	1.045HisPhe: 1.045 ± 0.019	1.487HisGly: 1.487 ± 0.027	0.631HisHis: 0.631 ± 0.019	1.443HisIle: 1.443 ± 0.025	0.855HisLys: 0.855 ± 0.022	2.091HisLeu: 2.091 ± 0.034	0.522HisMet: 0.522 ± 0.016	0.823HisAsn: 0.823 ± 0.019	1.156HisPro: 1.156 ± 0.023	0.873HisGln: 0.873 ± 0.022	1.157HisArg: 1.157 ± 0.028	1.245HisSer: 1.245 ± 0.026	1.106HisThr: 1.106 ± 0.024	1.387HisVal: 1.387 ± 0.027	0.327HisTrp: 0.327 ± 0.012	1.014HisTyr: 1.014 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.365IleAla: 6.365 ± 0.06	0.58IleCys: 0.58 ± 0.017	3.997IleAsp: 3.997 ± 0.046	4.44IleGlu: 4.44 ± 0.054	2.4IlePhe: 2.4 ± 0.035	5.217IleGly: 5.217 ± 0.061	1.435IleHis: 1.435 ± 0.026	4.585IleIle: 4.585 ± 0.061	3.125IleLys: 3.125 ± 0.048	5.321IleLeu: 5.321 ± 0.064	1.748IleMet: 1.748 ± 0.03	2.706IleAsn: 2.706 ± 0.038	2.94IlePro: 2.94 ± 0.037	2.403IleGln: 2.403 ± 0.033	3.728IleArg: 3.728 ± 0.044	4.452IleSer: 4.452 ± 0.05	3.759IleThr: 3.759 ± 0.05	5.358IleVal: 5.358 ± 0.054	0.764IleTrp: 0.764 ± 0.016	2.234IleTyr: 2.234 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.007LysAla: 4.007 ± 0.045	0.259LysCys: 0.259 ± 0.011	2.615LysAsp: 2.615 ± 0.045	4.105LysGlu: 4.105 ± 0.046	1.581LysPhe: 1.581 ± 0.028	3.267LysGly: 3.267 ± 0.04	1.131LysHis: 1.131 ± 0.021	3.197LysIle: 3.197 ± 0.047	2.956LysLys: 2.956 ± 0.044	5.478LysLeu: 5.478 ± 0.057	1.481LysMet: 1.481 ± 0.026	1.926LysAsn: 1.926 ± 0.035	2.176LysPro: 2.176 ± 0.033	2.736LysGln: 2.736 ± 0.041	2.822LysArg: 2.822 ± 0.036	2.921LysSer: 2.921 ± 0.039	2.757LysThr: 2.757 ± 0.034	3.482LysVal: 3.482 ± 0.043	0.726LysTrp: 0.726 ± 0.02	1.717LysTyr: 1.717 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	8.667LeuAla: 8.667 ± 0.074	0.784LeuCys: 0.784 ± 0.019	5.082LeuAsp: 5.082 ± 0.055	6.311LeuGlu: 6.311 ± 0.063	4.75LeuPhe: 4.75 ± 0.058	6.485LeuGly: 6.485 ± 0.06	2.299LeuHis: 2.299 ± 0.035	6.593LeuIle: 6.593 ± 0.067	5.033LeuLys: 5.033 ± 0.058	11.749LeuLeu: 11.749 ± 0.133	2.551LeuMet: 2.551 ± 0.035	4.044LeuAsn: 4.044 ± 0.043	4.547LeuPro: 4.547 ± 0.048	4.48LeuGln: 4.48 ± 0.047	4.981LeuArg: 4.981 ± 0.055	7.141LeuSer: 7.141 ± 0.066	5.606LeuThr: 5.606 ± 0.053	6.21LeuVal: 6.21 ± 0.059	1.111LeuTrp: 1.111 ± 0.022	3.237LeuTyr: 3.237 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.101MetAla: 2.101 ± 0.034	0.152MetCys: 0.152 ± 0.009	1.423MetAsp: 1.423 ± 0.023	1.757MetGlu: 1.757 ± 0.033	1.047MetPhe: 1.047 ± 0.022	1.637MetGly: 1.637 ± 0.029	0.507MetHis: 0.507 ± 0.015	1.972MetIle: 1.972 ± 0.031	1.955MetLys: 1.955 ± 0.03	3.05MetLeu: 3.05 ± 0.039	0.906MetMet: 0.906 ± 0.026	1.492MetAsn: 1.492 ± 0.026	1.108MetPro: 1.108 ± 0.027	1.096MetGln: 1.096 ± 0.022	1.445MetArg: 1.445 ± 0.028	1.743MetSer: 1.743 ± 0.028	1.678MetThr: 1.678 ± 0.029	1.668MetVal: 1.668 ± 0.03	0.26MetTrp: 0.26 ± 0.01	0.749MetTyr: 0.749 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.973AsnAla: 2.973 ± 0.041	0.29AsnCys: 0.29 ± 0.012	2.081AsnAsp: 2.081 ± 0.039	2.796AsnGlu: 2.796 ± 0.043	1.397AsnPhe: 1.397 ± 0.027	3.324AsnGly: 3.324 ± 0.04	0.834AsnHis: 0.834 ± 0.018	2.503AsnIle: 2.503 ± 0.037	2.257AsnLys: 2.257 ± 0.039	3.435AsnLeu: 3.435 ± 0.041	1.085AsnMet: 1.085 ± 0.022	1.933AsnAsn: 1.933 ± 0.043	1.968AsnPro: 1.968 ± 0.033	1.541AsnGln: 1.541 ± 0.026	2.21AsnArg: 2.21 ± 0.036	2.335AsnSer: 2.335 ± 0.041	2.13AsnThr: 2.13 ± 0.037	2.627AsnVal: 2.627 ± 0.041	0.642AsnTrp: 0.642 ± 0.017	1.64AsnTyr: 1.64 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	3.454ProAla: 3.454 ± 0.046	0.246ProCys: 0.246 ± 0.012	2.577ProAsp: 2.577 ± 0.035	3.15ProGlu: 3.15 ± 0.042	1.865ProPhe: 1.865 ± 0.03	2.911ProGly: 2.911 ± 0.046	0.9ProHis: 0.9 ± 0.021	2.454ProIle: 2.454 ± 0.036	1.54ProLys: 1.54 ± 0.025	4.108ProLeu: 4.108 ± 0.045	0.906ProMet: 0.906 ± 0.02	1.519ProAsn: 1.519 ± 0.032	1.294ProPro: 1.294 ± 0.026	1.436ProGln: 1.436 ± 0.025	1.429ProArg: 1.429 ± 0.029	2.458ProSer: 2.458 ± 0.036	1.898ProThr: 1.898 ± 0.037	3.099ProVal: 3.099 ± 0.036	0.501ProTrp: 0.501 ± 0.016	1.457ProTyr: 1.457 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.677GlnAla: 3.677 ± 0.05	0.248GlnCys: 0.248 ± 0.012	1.769GlnAsp: 1.769 ± 0.027	2.671GlnGlu: 2.671 ± 0.04	1.735GlnPhe: 1.735 ± 0.031	2.659GlnGly: 2.659 ± 0.04	1.003GlnHis: 1.003 ± 0.021	2.582GlnIle: 2.582 ± 0.037	1.823GlnLys: 1.823 ± 0.033	4.912GlnLeu: 4.912 ± 0.055	1.229GlnMet: 1.229 ± 0.024	1.391GlnAsn: 1.391 ± 0.028	1.742GlnPro: 1.742 ± 0.032	2.338GlnGln: 2.338 ± 0.043	2.134GlnArg: 2.134 ± 0.039	2.518GlnSer: 2.518 ± 0.037	2.19GlnThr: 2.19 ± 0.037	2.601GlnVal: 2.601 ± 0.034	0.647GlnTrp: 0.647 ± 0.017	1.455GlnTyr: 1.455 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	3.493ArgAla: 3.493 ± 0.043	0.398ArgCys: 0.398 ± 0.017	2.41ArgAsp: 2.41 ± 0.035	3.684ArgGlu: 3.684 ± 0.06	2.367ArgPhe: 2.367 ± 0.031	2.956ArgGly: 2.956 ± 0.039	1.132ArgHis: 1.132 ± 0.023	3.742ArgIle: 3.742 ± 0.039	2.94ArgLys: 2.94 ± 0.039	5.565ArgLeu: 5.565 ± 0.069	1.772ArgMet: 1.772 ± 0.031	2.143ArgAsn: 2.143 ± 0.029	1.71ArgPro: 1.71 ± 0.027	2.328ArgGln: 2.328 ± 0.035	2.789ArgArg: 2.789 ± 0.045	3.26ArgSer: 3.26 ± 0.04	2.675ArgThr: 2.675 ± 0.032	3.012ArgVal: 3.012 ± 0.038	0.716ArgTrp: 0.716 ± 0.016	1.947ArgTyr: 1.947 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	5.019SerAla: 5.019 ± 0.053	0.47SerCys: 0.47 ± 0.015	3.107SerAsp: 3.107 ± 0.043	3.93SerGlu: 3.93 ± 0.047	3.014SerPhe: 3.014 ± 0.045	5.625SerGly: 5.625 ± 0.056	1.246SerHis: 1.246 ± 0.024	4.256SerIle: 4.256 ± 0.05	3.154SerLys: 3.154 ± 0.041	6.341SerLeu: 6.341 ± 0.056	1.675SerMet: 1.675 ± 0.03	2.455SerAsn: 2.455 ± 0.042	2.429SerPro: 2.429 ± 0.038	2.159SerGln: 2.159 ± 0.033	3.163SerArg: 3.163 ± 0.041	4.393SerSer: 4.393 ± 0.049	3.139SerThr: 3.139 ± 0.042	4.183SerVal: 4.183 ± 0.047	0.909SerTrp: 0.909 ± 0.021	2.386SerTyr: 2.386 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	4.937ThrAla: 4.937 ± 0.056	0.33ThrCys: 0.33 ± 0.013	2.864ThrAsp: 2.864 ± 0.04	3.206ThrGlu: 3.206 ± 0.042	2.235ThrPhe: 2.235 ± 0.033	4.421ThrGly: 4.421 ± 0.071	0.989ThrHis: 0.989 ± 0.022	4.027ThrIle: 4.027 ± 0.048	2.403ThrLys: 2.403 ± 0.032	5.317ThrLeu: 5.317 ± 0.055	1.4ThrMet: 1.4 ± 0.025	1.977ThrAsn: 1.977 ± 0.034	2.325ThrPro: 2.325 ± 0.032	1.494ThrGln: 1.494 ± 0.027	2.24ThrArg: 2.24 ± 0.033	3.186ThrSer: 3.186 ± 0.044	2.746ThrThr: 2.746 ± 0.044	4.307ThrVal: 4.307 ± 0.048	0.614ThrTrp: 0.614 ± 0.018	1.801ThrTyr: 1.801 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	5.296ValAla: 5.296 ± 0.057	0.602ValCys: 0.602 ± 0.017	3.503ValAsp: 3.503 ± 0.046	4.337ValGlu: 4.337 ± 0.047	2.882ValPhe: 2.882 ± 0.039	4.344ValGly: 4.344 ± 0.053	1.445ValHis: 1.445 ± 0.029	4.871ValIle: 4.871 ± 0.053	3.607ValLys: 3.607 ± 0.044	6.962ValLeu: 6.962 ± 0.066	1.968ValMet: 1.968 ± 0.033	2.87ValAsn: 2.87 ± 0.043	2.866ValPro: 2.866 ± 0.039	2.747ValGln: 2.747 ± 0.037	3.448ValArg: 3.448 ± 0.048	4.598ValSer: 4.598 ± 0.048	4.053ValThr: 4.053 ± 0.054	4.696ValVal: 4.696 ± 0.053	0.888ValTrp: 0.888 ± 0.024	2.44ValTyr: 2.44 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	0.878TrpAla: 0.878 ± 0.021	0.097TrpCys: 0.097 ± 0.007	0.719TrpAsp: 0.719 ± 0.02	0.853TrpGlu: 0.853 ± 0.02	0.6TrpPhe: 0.6 ± 0.017	0.888TrpGly: 0.888 ± 0.022	0.332TrpHis: 0.332 ± 0.012	0.91TrpIle: 0.91 ± 0.021	0.693TrpLys: 0.693 ± 0.017	1.541TrpLeu: 1.541 ± 0.032	0.432TrpMet: 0.432 ± 0.014	0.708TrpAsn: 0.708 ± 0.018	0.388TrpPro: 0.388 ± 0.013	0.622TrpGln: 0.622 ± 0.018	0.729TrpArg: 0.729 ± 0.016	0.849TrpSer: 0.849 ± 0.019	0.71TrpThr: 0.71 ± 0.018	0.808TrpVal: 0.808 ± 0.022	0.193TrpTrp: 0.193 ± 0.012	0.429TrpTyr: 0.429 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.685TyrAla: 2.685 ± 0.04	0.325TyrCys: 0.325 ± 0.013	2.02TyrAsp: 2.02 ± 0.036	2.477TyrGlu: 2.477 ± 0.044	1.659TyrPhe: 1.659 ± 0.029	2.583TyrGly: 2.583 ± 0.036	0.757TyrHis: 0.757 ± 0.019	2.24TyrIle: 2.24 ± 0.031	1.704TyrLys: 1.704 ± 0.028	3.509TyrLeu: 3.509 ± 0.041	0.937TyrMet: 0.937 ± 0.022	1.51TyrAsn: 1.51 ± 0.031	1.55TyrPro: 1.55 ± 0.029	1.232TyrGln: 1.232 ± 0.025	2.107TyrArg: 2.107 ± 0.033	2.182TyrSer: 2.182 ± 0.033	1.867TyrThr: 1.867 ± 0.035	2.372TyrVal: 2.372 ± 0.031	0.469TyrTrp: 0.469 ± 0.014	1.516TyrTyr: 1.516 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.001

Statistics based on 6717 proteins (2160698 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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