Mycoplasma maculosum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 737 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449B422|A0A449B422_9MOLU Serine/threonine exchanger SteT OS=Mycoplasma maculosum OX=114885 GN=steT_2 PE=4 SV=1
MM1 pKa = 7.32NNNYY5 pKa = 10.42KK6 pKa = 10.71LIDD9 pKa = 3.67LHH11 pKa = 6.78GCTIDD16 pKa = 3.43EE17 pKa = 5.31AIAKK21 pKa = 9.91VSFEE25 pKa = 4.46LAFIDD30 pKa = 3.58NLEE33 pKa = 4.09NFTFEE38 pKa = 4.35IIVGKK43 pKa = 8.72GTGTLKK49 pKa = 10.54IFIEE53 pKa = 4.85NYY55 pKa = 10.68LIDD58 pKa = 3.9NNFNYY63 pKa = 10.49SYY65 pKa = 11.26DD66 pKa = 3.57EE67 pKa = 4.42NYY69 pKa = 10.58NSFFIKK75 pKa = 10.37INNNKK80 pKa = 9.38INLDD84 pKa = 3.61EE85 pKa = 5.19DD86 pKa = 3.47EE87 pKa = 5.0EE88 pKa = 5.23YY89 pKa = 11.46YY90 pKa = 11.22LNKK93 pKa = 10.21FKK95 pKa = 11.19

Molecular weight: 11.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449B3Z3|A0A449B3Z3_9MOLU Lipoprotein-associated type-17 domain-containing protein OS=Mycoplasma maculosum OX=114885 GN=NCTC10168_00239 PE=4 SV=1
MM1 pKa = 7.56AHH3 pKa = 6.1TKK5 pKa = 10.67AGGSTRR11 pKa = 11.84NGRR14 pKa = 11.84DD15 pKa = 2.93SRR17 pKa = 11.84GQRR20 pKa = 11.84LGIKK24 pKa = 10.22LGDD27 pKa = 3.89GQFCTAGSIIFRR39 pKa = 11.84QRR41 pKa = 11.84GTKK44 pKa = 9.68IFPGTNCGRR53 pKa = 11.84GNDD56 pKa = 3.75DD57 pKa = 3.47TLYY60 pKa = 11.44ALITGYY66 pKa = 11.17VKK68 pKa = 10.64FEE70 pKa = 3.64KK71 pKa = 10.25RR72 pKa = 11.84RR73 pKa = 11.84NRR75 pKa = 11.84TYY77 pKa = 11.07ASVYY81 pKa = 9.02PEE83 pKa = 3.65RR84 pKa = 11.84KK85 pKa = 9.76VEE87 pKa = 4.23VKK89 pKa = 10.41SSKK92 pKa = 10.74

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

737

0

737

272390

31

3806

369.6

42.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.683 ± 0.131

0.395 ± 0.019

5.421 ± 0.08

7.491 ± 0.093

5.454 ± 0.109

4.201 ± 0.099

1.147 ± 0.032

10.249 ± 0.108

10.662 ± 0.096

9.481 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.591 ± 0.043

9.455 ± 0.198

2.477 ± 0.054

2.621 ± 0.057

2.814 ± 0.059

6.838 ± 0.076

4.871 ± 0.067

4.939 ± 0.079

0.884 ± 0.023

4.326 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski