Amino acid dipepetide frequency for Roseobacter phage CRP-6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.351AlaAla: 6.351 ± 0.915
0.565AlaCys: 0.565 ± 0.199
5.857AlaAsp: 5.857 ± 0.671
5.151AlaGlu: 5.151 ± 0.68
3.317AlaPhe: 3.317 ± 0.455
6.986AlaGly: 6.986 ± 0.736
0.635AlaHis: 0.635 ± 0.208
4.728AlaIle: 4.728 ± 0.585
6.633AlaLys: 6.633 ± 0.876
7.692AlaLeu: 7.692 ± 0.699
2.399AlaMet: 2.399 ± 0.383
3.246AlaAsn: 3.246 ± 0.55
2.47AlaPro: 2.47 ± 0.385
2.47AlaGln: 2.47 ± 0.472
3.528AlaArg: 3.528 ± 0.496
4.305AlaSer: 4.305 ± 0.708
7.409AlaThr: 7.409 ± 2.102
5.292AlaVal: 5.292 ± 0.538
1.623AlaTrp: 1.623 ± 0.35
2.823AlaTyr: 2.823 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
0.494CysAla: 0.494 ± 0.219
0.141CysCys: 0.141 ± 0.105
0.917CysAsp: 0.917 ± 0.225
0.706CysGlu: 0.706 ± 0.254
0.423CysPhe: 0.423 ± 0.165
0.847CysGly: 0.847 ± 0.217
0.282CysHis: 0.282 ± 0.125
0.282CysIle: 0.282 ± 0.142
0.917CysLys: 0.917 ± 0.3
0.565CysLeu: 0.565 ± 0.226
0.141CysMet: 0.141 ± 0.111
0.423CysAsn: 0.423 ± 0.186
0.353CysPro: 0.353 ± 0.191
0.212CysGln: 0.212 ± 0.145
0.635CysArg: 0.635 ± 0.228
0.635CysSer: 0.635 ± 0.262
0.212CysThr: 0.212 ± 0.104
0.847CysVal: 0.847 ± 0.296
0.141CysTrp: 0.141 ± 0.097
0.282CysTyr: 0.282 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
5.081AspAla: 5.081 ± 0.646
0.423AspCys: 0.423 ± 0.168
4.587AspAsp: 4.587 ± 0.549
4.305AspGlu: 4.305 ± 0.53
2.046AspPhe: 2.046 ± 0.452
5.01AspGly: 5.01 ± 0.648
0.847AspHis: 0.847 ± 0.235
5.01AspIle: 5.01 ± 0.581
3.811AspLys: 3.811 ± 0.482
5.716AspLeu: 5.716 ± 0.576
2.329AspMet: 2.329 ± 0.398
3.317AspAsn: 3.317 ± 0.542
2.329AspPro: 2.329 ± 0.376
3.105AspGln: 3.105 ± 0.468
2.893AspArg: 2.893 ± 0.489
3.246AspSer: 3.246 ± 0.413
4.869AspThr: 4.869 ± 0.654
4.587AspVal: 4.587 ± 0.652
1.411AspTrp: 1.411 ± 0.31
2.47AspTyr: 2.47 ± 0.433
0.0AspXaa: 0.0 ± 0.0
Glu
5.151GluAla: 5.151 ± 0.599
0.494GluCys: 0.494 ± 0.231
4.305GluAsp: 4.305 ± 0.641
5.928GluGlu: 5.928 ± 1.088
1.905GluPhe: 1.905 ± 0.326
4.163GluGly: 4.163 ± 0.537
1.482GluHis: 1.482 ± 0.39
4.022GluIle: 4.022 ± 0.537
4.163GluLys: 4.163 ± 0.593
6.28GluLeu: 6.28 ± 0.782
1.694GluMet: 1.694 ± 0.403
2.329GluAsn: 2.329 ± 0.404
2.399GluPro: 2.399 ± 0.494
2.399GluGln: 2.399 ± 0.406
2.54GluArg: 2.54 ± 0.385
3.811GluSer: 3.811 ± 0.609
3.74GluThr: 3.74 ± 0.591
4.799GluVal: 4.799 ± 0.647
1.623GluTrp: 1.623 ± 0.372
2.258GluTyr: 2.258 ± 0.482
0.0GluXaa: 0.0 ± 0.0
Phe
2.54PheAla: 2.54 ± 0.576
0.423PheCys: 0.423 ± 0.17
3.458PheAsp: 3.458 ± 0.443
2.823PheGlu: 2.823 ± 0.543
1.552PhePhe: 1.552 ± 0.305
3.317PheGly: 3.317 ± 0.435
0.423PheHis: 0.423 ± 0.171
1.058PheIle: 1.058 ± 0.271
2.329PheLys: 2.329 ± 0.5
2.117PheLeu: 2.117 ± 0.384
1.411PheMet: 1.411 ± 0.271
1.764PheAsn: 1.764 ± 0.376
1.552PhePro: 1.552 ± 0.374
0.565PheGln: 0.565 ± 0.216
1.129PheArg: 1.129 ± 0.322
2.752PheSer: 2.752 ± 0.359
2.964PheThr: 2.964 ± 0.462
2.046PheVal: 2.046 ± 0.461
0.635PheTrp: 0.635 ± 0.189
1.764PheTyr: 1.764 ± 0.438
0.0PheXaa: 0.0 ± 0.0
Gly
8.962GlyAla: 8.962 ± 3.176
0.635GlyCys: 0.635 ± 0.208
6.28GlyAsp: 6.28 ± 0.884
5.363GlyGlu: 5.363 ± 0.736
2.823GlyPhe: 2.823 ± 0.432
4.022GlyGly: 4.022 ± 0.615
1.058GlyHis: 1.058 ± 0.27
4.516GlyIle: 4.516 ± 0.724
4.446GlyLys: 4.446 ± 0.635
5.151GlyLeu: 5.151 ± 0.595
2.117GlyMet: 2.117 ± 0.404
3.881GlyAsn: 3.881 ± 0.703
2.329GlyPro: 2.329 ± 0.486
3.105GlyGln: 3.105 ± 1.247
2.752GlyArg: 2.752 ± 0.483
6.563GlySer: 6.563 ± 0.747
6.422GlyThr: 6.422 ± 1.287
5.857GlyVal: 5.857 ± 0.667
1.058GlyTrp: 1.058 ± 0.279
2.54GlyTyr: 2.54 ± 0.476
0.0GlyXaa: 0.0 ± 0.0
His
1.411HisAla: 1.411 ± 0.275
0.565HisCys: 0.565 ± 0.257
1.058HisAsp: 1.058 ± 0.254
0.917HisGlu: 0.917 ± 0.241
0.706HisPhe: 0.706 ± 0.199
0.917HisGly: 0.917 ± 0.291
0.282HisHis: 0.282 ± 0.153
0.776HisIle: 0.776 ± 0.216
1.058HisLys: 1.058 ± 0.306
1.694HisLeu: 1.694 ± 0.265
0.423HisMet: 0.423 ± 0.158
0.635HisAsn: 0.635 ± 0.231
0.423HisPro: 0.423 ± 0.173
0.141HisGln: 0.141 ± 0.098
0.988HisArg: 0.988 ± 0.264
0.706HisSer: 0.706 ± 0.228
1.129HisThr: 1.129 ± 0.322
1.27HisVal: 1.27 ± 0.36
0.423HisTrp: 0.423 ± 0.151
1.2HisTyr: 1.2 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
3.599IleAla: 3.599 ± 0.503
0.494IleCys: 0.494 ± 0.189
3.669IleAsp: 3.669 ± 0.513
4.093IleGlu: 4.093 ± 0.596
1.835IlePhe: 1.835 ± 0.428
4.446IleGly: 4.446 ± 0.586
0.776IleHis: 0.776 ± 0.256
2.188IleIle: 2.188 ± 0.361
3.669IleLys: 3.669 ± 0.5
3.881IleLeu: 3.881 ± 0.633
1.27IleMet: 1.27 ± 0.296
2.258IleAsn: 2.258 ± 0.545
1.764IlePro: 1.764 ± 0.348
1.905IleGln: 1.905 ± 0.384
2.893IleArg: 2.893 ± 0.41
3.317IleSer: 3.317 ± 0.45
4.728IleThr: 4.728 ± 0.616
3.458IleVal: 3.458 ± 0.583
0.212IleTrp: 0.212 ± 0.116
1.058IleTyr: 1.058 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
5.504LysAla: 5.504 ± 0.955
0.353LysCys: 0.353 ± 0.177
3.952LysAsp: 3.952 ± 0.682
2.964LysGlu: 2.964 ± 0.56
2.117LysPhe: 2.117 ± 0.4
6.28LysGly: 6.28 ± 1.718
1.764LysHis: 1.764 ± 0.315
3.246LysIle: 3.246 ± 0.469
3.458LysLys: 3.458 ± 0.597
4.799LysLeu: 4.799 ± 0.607
1.623LysMet: 1.623 ± 0.376
1.694LysAsn: 1.694 ± 0.419
2.964LysPro: 2.964 ± 0.521
1.976LysGln: 1.976 ± 0.428
2.47LysArg: 2.47 ± 0.503
4.093LysSer: 4.093 ± 0.767
3.811LysThr: 3.811 ± 0.552
5.434LysVal: 5.434 ± 0.657
0.565LysTrp: 0.565 ± 0.198
1.905LysTyr: 1.905 ± 0.337
0.0LysXaa: 0.0 ± 0.0
Leu
6.21LeuAla: 6.21 ± 0.685
0.635LeuCys: 0.635 ± 0.361
5.857LeuAsp: 5.857 ± 0.558
5.857LeuGlu: 5.857 ± 0.642
2.752LeuPhe: 2.752 ± 0.46
6.845LeuGly: 6.845 ± 0.873
1.2LeuHis: 1.2 ± 0.293
2.964LeuIle: 2.964 ± 0.435
5.01LeuLys: 5.01 ± 0.624
5.434LeuLeu: 5.434 ± 0.779
2.117LeuMet: 2.117 ± 0.497
4.022LeuAsn: 4.022 ± 0.441
2.258LeuPro: 2.258 ± 0.418
1.976LeuGln: 1.976 ± 0.401
4.728LeuArg: 4.728 ± 0.547
6.21LeuSer: 6.21 ± 0.818
5.151LeuThr: 5.151 ± 0.57
4.022LeuVal: 4.022 ± 0.498
0.988LeuTrp: 0.988 ± 0.263
2.399LeuTyr: 2.399 ± 0.412
0.0LeuXaa: 0.0 ± 0.0
Met
2.329MetAla: 2.329 ± 0.374
0.494MetCys: 0.494 ± 0.222
1.764MetAsp: 1.764 ± 0.359
2.117MetGlu: 2.117 ± 0.386
0.706MetPhe: 0.706 ± 0.232
1.058MetGly: 1.058 ± 0.286
0.423MetHis: 0.423 ± 0.188
1.27MetIle: 1.27 ± 0.301
1.694MetLys: 1.694 ± 0.402
1.694MetLeu: 1.694 ± 0.451
0.565MetMet: 0.565 ± 0.221
0.776MetAsn: 0.776 ± 0.254
1.2MetPro: 1.2 ± 0.266
0.776MetGln: 0.776 ± 0.257
1.694MetArg: 1.694 ± 0.379
2.329MetSer: 2.329 ± 0.363
2.54MetThr: 2.54 ± 0.521
2.117MetVal: 2.117 ± 0.371
0.847MetTrp: 0.847 ± 0.233
1.058MetTyr: 1.058 ± 0.273
0.0MetXaa: 0.0 ± 0.0
Asn
2.964AsnAla: 2.964 ± 0.414
0.423AsnCys: 0.423 ± 0.161
2.964AsnAsp: 2.964 ± 0.452
2.329AsnGlu: 2.329 ± 0.433
1.764AsnPhe: 1.764 ± 0.329
4.587AsnGly: 4.587 ± 0.569
0.847AsnHis: 0.847 ± 0.236
2.893AsnIle: 2.893 ± 0.537
1.976AsnLys: 1.976 ± 0.41
3.387AsnLeu: 3.387 ± 0.458
1.129AsnMet: 1.129 ± 0.265
2.964AsnAsn: 2.964 ± 0.519
1.905AsnPro: 1.905 ± 0.389
1.27AsnGln: 1.27 ± 0.277
1.623AsnArg: 1.623 ± 0.336
3.387AsnSer: 3.387 ± 0.488
3.599AsnThr: 3.599 ± 0.503
3.175AsnVal: 3.175 ± 0.448
0.706AsnTrp: 0.706 ± 0.3
1.694AsnTyr: 1.694 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
3.034ProAla: 3.034 ± 0.369
0.423ProCys: 0.423 ± 0.164
2.399ProAsp: 2.399 ± 0.442
2.329ProGlu: 2.329 ± 0.353
1.411ProPhe: 1.411 ± 0.393
0.353ProGly: 0.353 ± 0.257
0.706ProHis: 0.706 ± 0.288
1.623ProIle: 1.623 ± 0.338
2.258ProLys: 2.258 ± 0.381
2.611ProLeu: 2.611 ± 0.498
0.494ProMet: 0.494 ± 0.176
1.552ProAsn: 1.552 ± 0.292
0.776ProPro: 0.776 ± 0.212
1.411ProGln: 1.411 ± 0.408
1.27ProArg: 1.27 ± 0.389
3.246ProSer: 3.246 ± 0.415
2.964ProThr: 2.964 ± 0.63
3.458ProVal: 3.458 ± 0.509
0.494ProTrp: 0.494 ± 0.213
1.764ProTyr: 1.764 ± 0.365
0.0ProXaa: 0.0 ± 0.0
Gln
2.964GlnAla: 2.964 ± 0.443
0.353GlnCys: 0.353 ± 0.167
1.552GlnAsp: 1.552 ± 0.423
1.623GlnGlu: 1.623 ± 0.3
1.27GlnPhe: 1.27 ± 0.321
2.823GlnGly: 2.823 ± 0.784
0.494GlnHis: 0.494 ± 0.17
2.611GlnIle: 2.611 ± 0.385
2.117GlnLys: 2.117 ± 0.905
2.54GlnLeu: 2.54 ± 0.507
0.988GlnMet: 0.988 ± 0.257
1.27GlnAsn: 1.27 ± 0.291
0.917GlnPro: 0.917 ± 0.219
1.482GlnGln: 1.482 ± 0.452
1.552GlnArg: 1.552 ± 0.369
2.47GlnSer: 2.47 ± 0.363
2.964GlnThr: 2.964 ± 0.581
2.258GlnVal: 2.258 ± 0.346
0.494GlnTrp: 0.494 ± 0.192
0.847GlnTyr: 0.847 ± 0.267
0.0GlnXaa: 0.0 ± 0.0
Arg
3.811ArgAla: 3.811 ± 0.525
0.353ArgCys: 0.353 ± 0.159
2.964ArgAsp: 2.964 ± 0.429
2.682ArgGlu: 2.682 ± 0.525
1.623ArgPhe: 1.623 ± 0.351
2.611ArgGly: 2.611 ± 0.472
0.988ArgHis: 0.988 ± 0.294
1.976ArgIle: 1.976 ± 0.356
2.47ArgLys: 2.47 ± 0.48
3.317ArgLeu: 3.317 ± 0.524
1.411ArgMet: 1.411 ± 0.328
1.976ArgAsn: 1.976 ± 0.353
1.482ArgPro: 1.482 ± 0.284
1.835ArgGln: 1.835 ± 0.386
2.188ArgArg: 2.188 ± 0.418
3.034ArgSer: 3.034 ± 0.393
2.117ArgThr: 2.117 ± 0.418
3.034ArgVal: 3.034 ± 0.554
0.706ArgTrp: 0.706 ± 0.2
2.258ArgTyr: 2.258 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
7.127SerAla: 7.127 ± 0.785
0.635SerCys: 0.635 ± 0.205
4.234SerAsp: 4.234 ± 0.472
3.669SerGlu: 3.669 ± 0.485
2.47SerPhe: 2.47 ± 0.536
5.928SerGly: 5.928 ± 0.733
1.129SerHis: 1.129 ± 0.34
3.175SerIle: 3.175 ± 0.581
3.952SerLys: 3.952 ± 0.573
6.21SerLeu: 6.21 ± 0.682
1.058SerMet: 1.058 ± 0.28
2.964SerAsn: 2.964 ± 0.48
2.188SerPro: 2.188 ± 0.344
2.188SerGln: 2.188 ± 0.389
3.246SerArg: 3.246 ± 0.473
3.952SerSer: 3.952 ± 0.793
5.151SerThr: 5.151 ± 0.745
4.799SerVal: 4.799 ± 0.579
1.482SerTrp: 1.482 ± 0.334
2.54SerTyr: 2.54 ± 0.433
0.0SerXaa: 0.0 ± 0.0
Thr
5.857ThrAla: 5.857 ± 0.707
0.282ThrCys: 0.282 ± 0.118
3.317ThrAsp: 3.317 ± 0.467
3.881ThrGlu: 3.881 ± 0.565
3.74ThrPhe: 3.74 ± 0.705
10.514ThrGly: 10.514 ± 3.532
1.27ThrHis: 1.27 ± 0.289
3.317ThrIle: 3.317 ± 0.659
2.611ThrLys: 2.611 ± 0.437
5.716ThrLeu: 5.716 ± 0.722
2.54ThrMet: 2.54 ± 0.597
3.881ThrAsn: 3.881 ± 0.46
3.246ThrPro: 3.246 ± 0.595
2.188ThrGln: 2.188 ± 0.467
1.835ThrArg: 1.835 ± 0.378
5.222ThrSer: 5.222 ± 0.822
5.01ThrThr: 5.01 ± 0.879
6.28ThrVal: 6.28 ± 0.771
1.058ThrTrp: 1.058 ± 0.282
2.964ThrTyr: 2.964 ± 0.462
0.0ThrXaa: 0.0 ± 0.0
Val
5.928ValAla: 5.928 ± 0.636
1.2ValCys: 1.2 ± 0.346
4.94ValAsp: 4.94 ± 0.634
5.151ValGlu: 5.151 ± 0.669
2.399ValPhe: 2.399 ± 0.404
5.716ValGly: 5.716 ± 0.91
1.129ValHis: 1.129 ± 0.268
3.175ValIle: 3.175 ± 0.465
4.516ValLys: 4.516 ± 0.515
4.234ValLeu: 4.234 ± 0.535
2.188ValMet: 2.188 ± 0.481
3.317ValAsn: 3.317 ± 0.621
2.258ValPro: 2.258 ± 0.342
2.47ValGln: 2.47 ± 0.427
3.175ValArg: 3.175 ± 0.556
5.222ValSer: 5.222 ± 0.745
6.21ValThr: 6.21 ± 0.922
5.928ValVal: 5.928 ± 0.742
0.847ValTrp: 0.847 ± 0.251
2.258ValTyr: 2.258 ± 0.471
0.0ValXaa: 0.0 ± 0.0
Trp
1.129TrpAla: 1.129 ± 0.307
0.212TrpCys: 0.212 ± 0.115
1.058TrpAsp: 1.058 ± 0.365
1.411TrpGlu: 1.411 ± 0.308
0.353TrpPhe: 0.353 ± 0.177
1.341TrpGly: 1.341 ± 0.298
0.212TrpHis: 0.212 ± 0.122
0.776TrpIle: 0.776 ± 0.211
1.27TrpLys: 1.27 ± 0.286
1.27TrpLeu: 1.27 ± 0.285
0.494TrpMet: 0.494 ± 0.212
0.847TrpAsn: 0.847 ± 0.213
0.353TrpPro: 0.353 ± 0.145
0.423TrpGln: 0.423 ± 0.134
0.706TrpArg: 0.706 ± 0.201
1.623TrpSer: 1.623 ± 0.402
0.776TrpThr: 0.776 ± 0.214
0.988TrpVal: 0.988 ± 0.259
0.282TrpTrp: 0.282 ± 0.122
0.776TrpTyr: 0.776 ± 0.233
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.105TyrAla: 3.105 ± 0.464
0.353TyrCys: 0.353 ± 0.149
2.258TyrAsp: 2.258 ± 0.428
2.188TyrGlu: 2.188 ± 0.39
1.411TyrPhe: 1.411 ± 0.274
2.117TyrGly: 2.117 ± 0.455
0.776TyrHis: 0.776 ± 0.245
1.905TyrIle: 1.905 ± 0.328
2.611TyrLys: 2.611 ± 0.434
2.329TyrLeu: 2.329 ± 0.362
0.988TyrMet: 0.988 ± 0.227
2.47TyrAsn: 2.47 ± 0.527
1.411TyrPro: 1.411 ± 0.309
1.694TyrGln: 1.694 ± 0.383
0.988TyrArg: 0.988 ± 0.253
1.976TyrSer: 1.976 ± 0.4
2.964TyrThr: 2.964 ± 0.538
2.611TyrVal: 2.611 ± 0.436
0.706TyrTrp: 0.706 ± 0.211
1.552TyrTyr: 1.552 ± 0.317
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (14172 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski