Peptostreptococcus stomatis DSM 17678

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Peptostreptococcus; Peptostreptococcus stomatis

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1600 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0E1A4|E0E1A4_9FIRM Leucine Rich Repeat protein OS=Peptostreptococcus stomatis DSM 17678 OX=596315 GN=HMPREF0634_0456 PE=3 SV=1
MM1 pKa = 7.35SKK3 pKa = 10.46EE4 pKa = 3.97LVKK7 pKa = 11.04DD8 pKa = 3.54RR9 pKa = 11.84VVEE12 pKa = 3.84YY13 pKa = 10.37LIQEE17 pKa = 4.25LGVPEE22 pKa = 4.12EE23 pKa = 4.47MIEE26 pKa = 3.79IDD28 pKa = 3.87TPLSEE33 pKa = 4.35YY34 pKa = 10.95EE35 pKa = 4.11EE36 pKa = 4.69GVEE39 pKa = 4.06GTIDD43 pKa = 3.21ITVTTEE49 pKa = 3.95DD50 pKa = 3.92EE51 pKa = 4.27EE52 pKa = 5.05GALLPLMIVVCLDD65 pKa = 3.51DD66 pKa = 6.59DD67 pKa = 4.16IEE69 pKa = 4.29LTEE72 pKa = 4.34SVVDD76 pKa = 4.02GQMDD80 pKa = 4.1FLEE83 pKa = 5.67LIDD86 pKa = 4.33EE87 pKa = 4.46STHH90 pKa = 4.83VGRR93 pKa = 11.84MILTNGDD100 pKa = 2.89QMMYY104 pKa = 10.87ADD106 pKa = 3.66WNGTEE111 pKa = 4.9LEE113 pKa = 4.59DD114 pKa = 5.33EE115 pKa = 4.4EE116 pKa = 5.61ALPNYY121 pKa = 10.15NQMLEE126 pKa = 4.08EE127 pKa = 4.08YY128 pKa = 10.02KK129 pKa = 10.82AVEE132 pKa = 4.05NEE134 pKa = 4.0YY135 pKa = 10.33KK136 pKa = 10.49KK137 pKa = 11.36YY138 pKa = 9.07MAEE141 pKa = 5.0HH142 pKa = 7.17PEE144 pKa = 4.2CDD146 pKa = 4.05CGCEE150 pKa = 4.04DD151 pKa = 3.91HH152 pKa = 6.93HH153 pKa = 7.39HH154 pKa = 5.54NHH156 pKa = 6.6

Molecular weight: 17.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0E1N4|E0E1N4_9FIRM Uncharacterized protein OS=Peptostreptococcus stomatis DSM 17678 OX=596315 GN=HMPREF0634_0959 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 8.57KK9 pKa = 7.61RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.25RR14 pKa = 11.84EE15 pKa = 3.66HH16 pKa = 6.29GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 10.24SPNGRR28 pKa = 11.84NVLKK32 pKa = 10.53RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.9GRR39 pKa = 11.84NRR41 pKa = 11.84LTHH44 pKa = 6.18

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1600

0

1600

532072

37

2967

332.5

37.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.246 ± 0.059

1.011 ± 0.024

6.678 ± 0.056

7.032 ± 0.079

4.093 ± 0.043

6.827 ± 0.05

1.425 ± 0.021

8.763 ± 0.063

8.59 ± 0.06

9.089 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.909 ± 0.029

5.196 ± 0.053

2.862 ± 0.027

2.585 ± 0.027

4.216 ± 0.043

6.347 ± 0.055

4.674 ± 0.038

6.734 ± 0.051

0.612 ± 0.016

4.11 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski