Amino acid dipepetide frequency for Peptostreptococcus stomatis DSM 17678

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.909AlaAla: 3.909 ± 0.111
0.848AlaCys: 0.848 ± 0.04
3.723AlaAsp: 3.723 ± 0.092
3.477AlaGlu: 3.477 ± 0.081
2.596AlaPhe: 2.596 ± 0.076
4.712AlaGly: 4.712 ± 0.113
0.962AlaHis: 0.962 ± 0.044
5.781AlaIle: 5.781 ± 0.123
5.366AlaLys: 5.366 ± 0.121
5.798AlaLeu: 5.798 ± 0.113
2.056AlaMet: 2.056 ± 0.079
3.114AlaAsn: 3.114 ± 0.074
1.442AlaPro: 1.442 ± 0.064
1.695AlaGln: 1.695 ± 0.058
2.928AlaArg: 2.928 ± 0.087
4.082AlaSer: 4.082 ± 0.096
3.039AlaThr: 3.039 ± 0.087
4.075AlaVal: 4.075 ± 0.101
0.374AlaTrp: 0.374 ± 0.027
2.479AlaTyr: 2.479 ± 0.069
0.0AlaXaa: 0.0 ± 0.0
Cys
0.6CysAla: 0.6 ± 0.032
0.122CysCys: 0.122 ± 0.02
0.667CysAsp: 0.667 ± 0.037
0.543CysGlu: 0.543 ± 0.035
0.385CysPhe: 0.385 ± 0.028
1.03CysGly: 1.03 ± 0.046
0.239CysHis: 0.239 ± 0.022
0.866CysIle: 0.866 ± 0.041
0.765CysLys: 0.765 ± 0.04
0.88CysLeu: 0.88 ± 0.044
0.274CysMet: 0.274 ± 0.021
0.447CysAsn: 0.447 ± 0.03
0.417CysPro: 0.417 ± 0.027
0.368CysGln: 0.368 ± 0.028
0.391CysArg: 0.391 ± 0.031
0.626CysSer: 0.626 ± 0.032
0.462CysThr: 0.462 ± 0.033
0.624CysVal: 0.624 ± 0.037
0.07CysTrp: 0.07 ± 0.012
0.333CysTyr: 0.333 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.37AspAla: 3.37 ± 0.081
0.583AspCys: 0.583 ± 0.033
3.646AspAsp: 3.646 ± 0.105
4.907AspGlu: 4.907 ± 0.107
3.122AspPhe: 3.122 ± 0.081
4.139AspGly: 4.139 ± 0.083
1.037AspHis: 1.037 ± 0.054
6.507AspIle: 6.507 ± 0.128
6.336AspLys: 6.336 ± 0.142
6.881AspLeu: 6.881 ± 0.134
2.278AspMet: 2.278 ± 0.066
2.947AspAsn: 2.947 ± 0.074
1.787AspPro: 1.787 ± 0.056
2.227AspGln: 2.227 ± 0.079
3.173AspArg: 3.173 ± 0.087
3.605AspSer: 3.605 ± 0.089
2.83AspThr: 2.83 ± 0.076
3.765AspVal: 3.765 ± 0.089
0.474AspTrp: 0.474 ± 0.035
3.171AspTyr: 3.171 ± 0.08
0.0AspXaa: 0.0 ± 0.0
Glu
4.753GluAla: 4.753 ± 0.11
0.571GluCys: 0.571 ± 0.04
5.099GluAsp: 5.099 ± 0.116
6.182GluGlu: 6.182 ± 0.143
2.706GluPhe: 2.706 ± 0.072
4.163GluGly: 4.163 ± 0.096
0.928GluHis: 0.928 ± 0.043
6.242GluIle: 6.242 ± 0.126
6.871GluLys: 6.871 ± 0.145
5.911GluLeu: 5.911 ± 0.131
2.019GluMet: 2.019 ± 0.062
4.608GluAsn: 4.608 ± 0.105
1.4GluPro: 1.4 ± 0.052
1.28GluGln: 1.28 ± 0.051
3.0GluArg: 3.0 ± 0.079
3.571GluSer: 3.571 ± 0.091
2.763GluThr: 2.763 ± 0.076
4.909GluVal: 4.909 ± 0.115
0.376GluTrp: 0.376 ± 0.029
2.97GluTyr: 2.97 ± 0.08
0.0GluXaa: 0.0 ± 0.0
Phe
2.661PheAla: 2.661 ± 0.075
0.491PheCys: 0.491 ± 0.031
3.08PheAsp: 3.08 ± 0.078
2.941PheGlu: 2.941 ± 0.08
1.812PhePhe: 1.812 ± 0.063
2.75PheGly: 2.75 ± 0.082
0.455PheHis: 0.455 ± 0.033
3.605PheIle: 3.605 ± 0.095
3.282PheLys: 3.282 ± 0.086
3.56PheLeu: 3.56 ± 0.095
1.216PheMet: 1.216 ± 0.055
1.985PheAsn: 1.985 ± 0.07
1.163PhePro: 1.163 ± 0.044
0.866PheGln: 0.866 ± 0.042
1.477PheArg: 1.477 ± 0.049
2.947PheSer: 2.947 ± 0.081
2.06PheThr: 2.06 ± 0.068
2.753PheVal: 2.753 ± 0.08
0.273PheTrp: 0.273 ± 0.027
1.551PheTyr: 1.551 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
4.186GlyAla: 4.186 ± 0.113
0.763GlyCys: 0.763 ± 0.041
3.875GlyAsp: 3.875 ± 0.093
3.889GlyGlu: 3.889 ± 0.085
2.909GlyPhe: 2.909 ± 0.079
4.932GlyGly: 4.932 ± 0.117
1.225GlyHis: 1.225 ± 0.055
6.433GlyIle: 6.433 ± 0.116
5.866GlyLys: 5.866 ± 0.106
6.678GlyLeu: 6.678 ± 0.119
2.058GlyMet: 2.058 ± 0.07
3.037GlyAsn: 3.037 ± 0.081
1.53GlyPro: 1.53 ± 0.058
2.643GlyGln: 2.643 ± 0.071
2.877GlyArg: 2.877 ± 0.069
3.943GlySer: 3.943 ± 0.081
3.219GlyThr: 3.219 ± 0.091
4.633GlyVal: 4.633 ± 0.107
0.468GlyTrp: 0.468 ± 0.036
3.107GlyTyr: 3.107 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
0.793HisAla: 0.793 ± 0.037
0.154HisCys: 0.154 ± 0.018
0.831HisAsp: 0.831 ± 0.042
0.913HisGlu: 0.913 ± 0.039
0.647HisPhe: 0.647 ± 0.031
0.962HisGly: 0.962 ± 0.047
0.316HisHis: 0.316 ± 0.03
1.323HisIle: 1.323 ± 0.053
1.135HisLys: 1.135 ± 0.046
1.254HisLeu: 1.254 ± 0.045
0.462HisMet: 0.462 ± 0.031
0.682HisAsn: 0.682 ± 0.031
0.656HisPro: 0.656 ± 0.04
0.398HisGln: 0.398 ± 0.026
0.656HisArg: 0.656 ± 0.037
0.896HisSer: 0.896 ± 0.045
0.771HisThr: 0.771 ± 0.035
0.772HisVal: 0.772 ± 0.039
0.094HisTrp: 0.094 ± 0.014
0.536HisTyr: 0.536 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.486IleAla: 5.486 ± 0.112
1.007IleCys: 1.007 ± 0.043
6.322IleAsp: 6.322 ± 0.108
6.409IleGlu: 6.409 ± 0.132
3.654IlePhe: 3.654 ± 0.101
6.044IleGly: 6.044 ± 0.116
1.173IleHis: 1.173 ± 0.049
7.136IleIle: 7.136 ± 0.145
6.984IleLys: 6.984 ± 0.137
8.247IleLeu: 8.247 ± 0.154
2.263IleMet: 2.263 ± 0.071
4.588IleAsn: 4.588 ± 0.102
2.851IlePro: 2.851 ± 0.078
1.925IleGln: 1.925 ± 0.056
3.642IleArg: 3.642 ± 0.093
6.546IleSer: 6.546 ± 0.124
3.734IleThr: 3.734 ± 0.089
5.928IleVal: 5.928 ± 0.128
0.389IleTrp: 0.389 ± 0.028
3.297IleTyr: 3.297 ± 0.089
0.0IleXaa: 0.0 ± 0.0
Lys
5.255LysAla: 5.255 ± 0.119
0.626LysCys: 0.626 ± 0.031
6.142LysAsp: 6.142 ± 0.12
6.992LysGlu: 6.992 ± 0.151
3.097LysPhe: 3.097 ± 0.072
4.796LysGly: 4.796 ± 0.111
1.06LysHis: 1.06 ± 0.041
7.01LysIle: 7.01 ± 0.145
8.399LysLys: 8.399 ± 0.171
7.522LysLeu: 7.522 ± 0.136
2.622LysMet: 2.622 ± 0.073
5.467LysAsn: 5.467 ± 0.111
2.229LysPro: 2.229 ± 0.064
1.981LysGln: 1.981 ± 0.069
3.329LysArg: 3.329 ± 0.087
5.251LysSer: 5.251 ± 0.111
4.204LysThr: 4.204 ± 0.089
5.366LysVal: 5.366 ± 0.096
0.509LysTrp: 0.509 ± 0.035
4.043LysTyr: 4.043 ± 0.095
0.0LysXaa: 0.0 ± 0.0
Leu
6.678LeuAla: 6.678 ± 0.144
0.821LeuCys: 0.821 ± 0.041
6.68LeuAsp: 6.68 ± 0.136
7.127LeuGlu: 7.127 ± 0.151
3.184LeuPhe: 3.184 ± 0.094
6.473LeuGly: 6.473 ± 0.117
1.116LeuHis: 1.116 ± 0.042
6.999LeuIle: 6.999 ± 0.134
7.463LeuLys: 7.463 ± 0.145
7.723LeuLeu: 7.723 ± 0.14
2.671LeuMet: 2.671 ± 0.076
4.667LeuAsn: 4.667 ± 0.114
2.768LeuPro: 2.768 ± 0.079
1.671LeuGln: 1.671 ± 0.06
3.539LeuArg: 3.539 ± 0.094
6.416LeuSer: 6.416 ± 0.138
4.272LeuThr: 4.272 ± 0.088
7.01LeuVal: 7.01 ± 0.135
0.509LeuTrp: 0.509 ± 0.028
3.107LeuTyr: 3.107 ± 0.083
0.0LeuXaa: 0.0 ± 0.0
Met
2.274MetAla: 2.274 ± 0.088
0.32MetCys: 0.32 ± 0.027
2.19MetAsp: 2.19 ± 0.064
2.049MetGlu: 2.049 ± 0.06
0.912MetPhe: 0.912 ± 0.042
2.05MetGly: 2.05 ± 0.068
0.308MetHis: 0.308 ± 0.025
2.297MetIle: 2.297 ± 0.073
2.624MetLys: 2.624 ± 0.069
2.244MetLeu: 2.244 ± 0.074
0.863MetMet: 0.863 ± 0.044
1.567MetAsn: 1.567 ± 0.055
0.915MetPro: 0.915 ± 0.044
0.631MetGln: 0.631 ± 0.034
1.079MetArg: 1.079 ± 0.042
2.022MetSer: 2.022 ± 0.062
1.594MetThr: 1.594 ± 0.055
2.131MetVal: 2.131 ± 0.072
0.141MetTrp: 0.141 ± 0.015
0.878MetTyr: 0.878 ± 0.042
0.0MetXaa: 0.0 ± 0.0
Asn
2.753AsnAla: 2.753 ± 0.077
0.44AsnCys: 0.44 ± 0.03
2.812AsnAsp: 2.812 ± 0.086
3.191AsnGlu: 3.191 ± 0.078
2.255AsnPhe: 2.255 ± 0.075
3.251AsnGly: 3.251 ± 0.086
0.718AsnHis: 0.718 ± 0.04
5.482AsnIle: 5.482 ± 0.116
4.862AsnLys: 4.862 ± 0.113
4.879AsnLeu: 4.879 ± 0.099
1.571AsnMet: 1.571 ± 0.055
2.806AsnAsn: 2.806 ± 0.092
2.02AsnPro: 2.02 ± 0.065
1.492AsnGln: 1.492 ± 0.057
2.293AsnArg: 2.293 ± 0.071
2.979AsnSer: 2.979 ± 0.093
2.688AsnThr: 2.688 ± 0.081
2.964AsnVal: 2.964 ± 0.074
0.363AsnTrp: 0.363 ± 0.03
2.143AsnTyr: 2.143 ± 0.075
0.0AsnXaa: 0.0 ± 0.0
Pro
1.69ProAla: 1.69 ± 0.056
0.299ProCys: 0.299 ± 0.022
2.107ProAsp: 2.107 ± 0.068
2.186ProGlu: 2.186 ± 0.069
1.284ProPhe: 1.284 ± 0.053
2.05ProGly: 2.05 ± 0.063
0.489ProHis: 0.489 ± 0.031
2.485ProIle: 2.485 ± 0.07
2.06ProLys: 2.06 ± 0.06
2.263ProLeu: 2.263 ± 0.057
0.784ProMet: 0.784 ± 0.042
1.466ProAsn: 1.466 ± 0.052
0.579ProPro: 0.579 ± 0.033
0.818ProGln: 0.818 ± 0.04
1.075ProArg: 1.075 ± 0.051
1.87ProSer: 1.87 ± 0.066
1.507ProThr: 1.507 ± 0.053
2.276ProVal: 2.276 ± 0.072
0.188ProTrp: 0.188 ± 0.021
1.15ProTyr: 1.15 ± 0.051
0.0ProXaa: 0.0 ± 0.0
Gln
2.135GlnAla: 2.135 ± 0.082
0.195GlnCys: 0.195 ± 0.02
1.799GlnAsp: 1.799 ± 0.058
2.094GlnGlu: 2.094 ± 0.064
0.921GlnPhe: 0.921 ± 0.04
1.707GlnGly: 1.707 ± 0.051
0.303GlnHis: 0.303 ± 0.025
2.233GlnIle: 2.233 ± 0.058
2.071GlnLys: 2.071 ± 0.068
2.205GlnLeu: 2.205 ± 0.079
0.81GlnMet: 0.81 ± 0.035
1.218GlnAsn: 1.218 ± 0.047
0.69GlnPro: 0.69 ± 0.043
0.59GlnGln: 0.59 ± 0.035
1.054GlnArg: 1.054 ± 0.046
1.316GlnSer: 1.316 ± 0.049
1.156GlnThr: 1.156 ± 0.044
2.178GlnVal: 2.178 ± 0.069
0.171GlnTrp: 0.171 ± 0.017
1.009GlnTyr: 1.009 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
2.535ArgAla: 2.535 ± 0.066
0.366ArgCys: 0.366 ± 0.027
2.9ArgAsp: 2.9 ± 0.082
3.161ArgGlu: 3.161 ± 0.087
1.68ArgPhe: 1.68 ± 0.059
2.659ArgGly: 2.659 ± 0.072
0.684ArgHis: 0.684 ± 0.039
3.614ArgIle: 3.614 ± 0.093
3.353ArgLys: 3.353 ± 0.087
3.971ArgLeu: 3.971 ± 0.09
1.141ArgMet: 1.141 ± 0.045
2.133ArgAsn: 2.133 ± 0.065
1.329ArgPro: 1.329 ± 0.051
1.419ArgGln: 1.419 ± 0.054
1.825ArgArg: 1.825 ± 0.067
2.334ArgSer: 2.334 ± 0.068
1.844ArgThr: 1.844 ± 0.067
3.054ArgVal: 3.054 ± 0.062
0.284ArgTrp: 0.284 ± 0.029
1.866ArgTyr: 1.866 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
3.306SerAla: 3.306 ± 0.079
0.611SerCys: 0.611 ± 0.033
3.714SerAsp: 3.714 ± 0.094
3.558SerGlu: 3.558 ± 0.088
2.725SerPhe: 2.725 ± 0.073
4.87SerGly: 4.87 ± 0.106
1.037SerHis: 1.037 ± 0.042
5.928SerIle: 5.928 ± 0.123
5.479SerLys: 5.479 ± 0.126
6.441SerLeu: 6.441 ± 0.122
1.904SerMet: 1.904 ± 0.066
3.157SerAsn: 3.157 ± 0.096
1.755SerPro: 1.755 ± 0.058
2.143SerGln: 2.143 ± 0.065
2.761SerArg: 2.761 ± 0.083
4.154SerSer: 4.154 ± 0.109
2.877SerThr: 2.877 ± 0.069
3.998SerVal: 3.998 ± 0.092
0.368SerTrp: 0.368 ± 0.028
2.688SerTyr: 2.688 ± 0.073
0.0SerXaa: 0.0 ± 0.0
Thr
2.932ThrAla: 2.932 ± 0.084
0.481ThrCys: 0.481 ± 0.034
2.926ThrAsp: 2.926 ± 0.075
2.601ThrGlu: 2.601 ± 0.079
1.878ThrPhe: 1.878 ± 0.066
3.817ThrGly: 3.817 ± 0.106
0.746ThrHis: 0.746 ± 0.041
4.217ThrIle: 4.217 ± 0.106
3.426ThrLys: 3.426 ± 0.097
4.016ThrLeu: 4.016 ± 0.085
1.124ThrMet: 1.124 ± 0.042
2.398ThrAsn: 2.398 ± 0.067
1.618ThrPro: 1.618 ± 0.068
1.064ThrGln: 1.064 ± 0.046
2.154ThrArg: 2.154 ± 0.065
3.548ThrSer: 3.548 ± 0.078
2.265ThrThr: 2.265 ± 0.08
3.342ThrVal: 3.342 ± 0.103
0.306ThrTrp: 0.306 ± 0.028
1.881ThrTyr: 1.881 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
4.575ValAla: 4.575 ± 0.11
0.812ValCys: 0.812 ± 0.041
4.964ValAsp: 4.964 ± 0.104
4.868ValGlu: 4.868 ± 0.105
3.08ValPhe: 3.08 ± 0.082
4.719ValGly: 4.719 ± 0.122
0.81ValHis: 0.81 ± 0.041
5.281ValIle: 5.281 ± 0.117
5.302ValLys: 5.302 ± 0.103
6.317ValLeu: 6.317 ± 0.139
1.669ValMet: 1.669 ± 0.053
3.265ValAsn: 3.265 ± 0.088
1.972ValPro: 1.972 ± 0.057
1.314ValGln: 1.314 ± 0.055
2.71ValArg: 2.71 ± 0.082
4.498ValSer: 4.498 ± 0.092
3.133ValThr: 3.133 ± 0.092
4.949ValVal: 4.949 ± 0.134
0.419ValTrp: 0.419 ± 0.029
2.686ValTyr: 2.686 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
0.359TrpAla: 0.359 ± 0.025
0.113TrpCys: 0.113 ± 0.013
0.46TrpAsp: 0.46 ± 0.035
0.378TrpGlu: 0.378 ± 0.026
0.261TrpPhe: 0.261 ± 0.025
0.385TrpGly: 0.385 ± 0.022
0.094TrpHis: 0.094 ± 0.015
0.568TrpIle: 0.568 ± 0.034
0.459TrpLys: 0.459 ± 0.033
0.543TrpLeu: 0.543 ± 0.036
0.192TrpMet: 0.192 ± 0.019
0.325TrpAsn: 0.325 ± 0.026
0.147TrpPro: 0.147 ± 0.018
0.205TrpGln: 0.205 ± 0.02
0.226TrpArg: 0.226 ± 0.022
0.363TrpSer: 0.363 ± 0.023
0.31TrpThr: 0.31 ± 0.022
0.361TrpVal: 0.361 ± 0.032
0.066TrpTrp: 0.066 ± 0.012
0.31TrpTyr: 0.31 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.206TyrAla: 2.206 ± 0.068
0.487TyrCys: 0.487 ± 0.033
2.845TyrAsp: 2.845 ± 0.086
2.857TyrGlu: 2.857 ± 0.085
1.819TyrPhe: 1.819 ± 0.061
2.761TyrGly: 2.761 ± 0.058
0.551TyrHis: 0.551 ± 0.032
3.618TyrIle: 3.618 ± 0.082
3.798TyrLys: 3.798 ± 0.083
3.563TyrLeu: 3.563 ± 0.092
1.111TyrMet: 1.111 ± 0.042
2.084TyrAsn: 2.084 ± 0.068
1.355TyrPro: 1.355 ± 0.053
1.124TyrGln: 1.124 ± 0.051
1.968TyrArg: 1.968 ± 0.061
2.505TyrSer: 2.505 ± 0.067
2.013TyrThr: 2.013 ± 0.062
2.253TyrVal: 2.253 ± 0.054
0.282TyrTrp: 0.282 ± 0.029
1.898TyrTyr: 1.898 ± 0.075
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1600 proteins (532072 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski