Actinoplanes awajinensis subsp. mycoplanecinus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Actinoplanes; Actinoplanes awajinensis

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9641 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A101JTP2|A0A101JTP2_9ACTN Uncharacterized protein OS=Actinoplanes awajinensis subsp. mycoplanecinus OX=135947 GN=ADL15_19070 PE=4 SV=1
MM1 pKa = 7.04TRR3 pKa = 11.84AGLASIAVTALVLAGASSPVWAATPAMTLSSVTGPSGGGTIITGTVASPFVVFPAGTTPAVQFQYY68 pKa = 11.13VGTGSTTCSATAGAVAQIAVTGTTGTDD95 pKa = 3.4GVQTVDD101 pKa = 3.21PDD103 pKa = 3.28QVRR106 pKa = 11.84RR107 pKa = 11.84VSATKK112 pKa = 10.65VLFTVPSQAYY122 pKa = 8.36PVIGEE127 pKa = 4.54DD128 pKa = 4.86GYY130 pKa = 10.32PSTINPTGVVLAGAQTWAKK149 pKa = 10.1WNVCVYY155 pKa = 9.47DD156 pKa = 3.52TDD158 pKa = 4.1STVSSTLLATASYY171 pKa = 9.48TVTARR176 pKa = 11.84PTITSIIPASSPAGGGQSITVNGTGFSAVTTATTGAIGDD215 pKa = 3.98VPLVDD220 pKa = 4.61IKK222 pKa = 11.09VAPNGNSLTATTGPRR237 pKa = 11.84ASGTGLALTLTTPGGTVSSLDD258 pKa = 4.08PDD260 pKa = 4.92NNDD263 pKa = 3.17QPEE266 pKa = 4.33DD267 pKa = 4.2ADD269 pKa = 4.83PDD271 pKa = 3.91TDD273 pKa = 3.8DD274 pKa = 4.68APIGFLYY281 pKa = 11.09SNGITIAPRR290 pKa = 11.84TAPAGTTVVVDD301 pKa = 3.36VNGAGFSGLSFSAAGDD317 pKa = 3.76PQSTQAHH324 pKa = 5.22VFLVRR329 pKa = 11.84DD330 pKa = 4.28AYY332 pKa = 11.49VLDD335 pKa = 4.04SNRR338 pKa = 11.84GVAEE342 pKa = 4.24CVVQAVIGDD351 pKa = 3.9TEE353 pKa = 5.11LICTLDD359 pKa = 3.76LSADD363 pKa = 3.78QLSLTDD369 pKa = 4.2SSTLPSTPITDD380 pKa = 3.31GAYY383 pKa = 10.32FLTVVADD390 pKa = 4.66GSTTALNPNSSIVSSGAAFIVAPYY414 pKa = 10.27

Molecular weight: 41.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A101JH42|A0A101JH42_9ACTN Polysaccharide deacetylase OS=Actinoplanes awajinensis subsp. mycoplanecinus OX=135947 GN=ADL15_37445 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84AIASGSHH9 pKa = 5.01SWRR12 pKa = 11.84GSSLSMRR19 pKa = 11.84AHH21 pKa = 6.09GRR23 pKa = 11.84LGSLLLMRR31 pKa = 11.84AHH33 pKa = 6.56GRR35 pKa = 11.84LGSLLLMRR43 pKa = 11.84GRR45 pKa = 11.84SRR47 pKa = 11.84RR48 pKa = 11.84GNSLPMGGVVRR59 pKa = 11.84MSGG62 pKa = 2.9

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9641

0

9641

3188279

29

4165

330.7

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.035 ± 0.038

0.697 ± 0.006

6.025 ± 0.02

4.878 ± 0.025

2.779 ± 0.016

9.284 ± 0.024

2.136 ± 0.011

3.619 ± 0.017

1.881 ± 0.019

10.542 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.699 ± 0.01

1.902 ± 0.017

6.0 ± 0.024

2.855 ± 0.015

7.756 ± 0.031

5.042 ± 0.022

6.56 ± 0.031

8.649 ± 0.021

1.591 ± 0.011

2.072 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski