Apaloderma vittatum (Bar-tailed trogon)
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7903 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A091NNV1|A0A091NNV1_APAVI Uncharacterized protein (Fragment) OS=Apaloderma vittatum OX=57397 GN=N311_05124 PE=4 SV=1
SS1 pKa = 7.12 RR2 pKa = 11.84 LTWLEE7 pKa = 3.51 NSGVGFSLDD16 pKa = 3.46 YY17 pKa = 9.24 PTISLHH23 pKa = 6.08 AVSRR27 pKa = 11.84 DD28 pKa = 3.0 LSAYY32 pKa = 8.18 PWEE35 pKa = 4.42 HH36 pKa = 6.79 LYY38 pKa = 11.74 VMVNAKK44 pKa = 10.3 FEE46 pKa = 4.48 EE47 pKa = 4.52 EE48 pKa = 4.12 TKK50 pKa = 10.07 EE51 pKa = 4.02 APVAEE56 pKa = 4.68 GEE58 pKa = 4.41 EE59 pKa = 4.46 EE60 pKa = 4.74 DD61 pKa = 6.13 DD62 pKa = 5.21 SDD64 pKa = 6.0 DD65 pKa = 4.07 DD66 pKa = 4.36 SEE68 pKa = 5.15 PIAEE72 pKa = 4.16 FRR74 pKa = 11.84 FVPSDD79 pKa = 3.36 KK80 pKa = 10.88 SALEE84 pKa = 3.78 AMFAAMCEE92 pKa = 4.38 CQALHH97 pKa = 7.41 PDD99 pKa = 4.21 PDD101 pKa = 5.77 DD102 pKa = 4.05 EE103 pKa = 7.61 DD104 pKa = 4.46 SDD106 pKa = 3.76 NDD108 pKa = 3.97 YY109 pKa = 11.37 EE110 pKa = 4.59 GEE112 pKa = 4.16 EE113 pKa = 4.41 YY114 pKa = 10.56 DD115 pKa = 4.4 VEE117 pKa = 4.26 AHH119 pKa = 5.75 EE120 pKa = 5.4 LEE122 pKa = 5.33 HH123 pKa = 8.4 GDD125 pKa = 3.1 IPSFYY130 pKa = 9.42 TYY132 pKa = 11.03 EE133 pKa = 4.17 EE134 pKa = 4.58 GLSHH138 pKa = 6.9 LTAEE142 pKa = 4.71 GQATLEE148 pKa = 4.11 RR149 pKa = 11.84 LEE151 pKa = 4.74 GMLAQSVSSQYY162 pKa = 11.66 NMAGVRR168 pKa = 11.84 TEE170 pKa = 3.88 DD171 pKa = 3.84 SIRR174 pKa = 11.84 EE175 pKa = 4.08 YY176 pKa = 10.91 EE177 pKa = 4.67 DD178 pKa = 3.35 GMEE181 pKa = 4.02 VDD183 pKa = 3.84 VAPVVAGQFEE193 pKa = 4.42 DD194 pKa = 5.26 AEE196 pKa = 4.32 VDD198 pKa = 3.59 HH199 pKa = 7.08
Molecular weight: 22.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.617
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.656
Rodwell 3.63
Grimsley 3.528
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.668
Sillero 3.923
Patrickios 1.202
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A091NKP0|A0A091NKP0_APAVI 6-phosphofructo-2-kinase/fructose-2 6-bisphosphatase 2 (Fragment) OS=Apaloderma vittatum OX=57397 GN=N311_11513 PE=4 SV=1
GG1 pKa = 7.26 KK2 pKa = 10.29 GRR4 pKa = 11.84 EE5 pKa = 4.21 GKK7 pKa = 10.25 GRR9 pKa = 11.84 AGKK12 pKa = 10.14 GRR14 pKa = 11.84 AGKK17 pKa = 10.12 GRR19 pKa = 11.84 AGKK22 pKa = 10.12 GRR24 pKa = 11.84 AGKK27 pKa = 10.12 GRR29 pKa = 11.84 AGKK32 pKa = 10.12 GRR34 pKa = 11.84 AGKK37 pKa = 10.12 GRR39 pKa = 11.84 AGKK42 pKa = 10.12 GRR44 pKa = 11.84 AGKK47 pKa = 10.12 GRR49 pKa = 11.84 AGKK52 pKa = 10.14 GRR54 pKa = 11.84 AGKK57 pKa = 10.28 GG58 pKa = 3.09
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 10.935
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.515
Grimsley 12.72
Solomon 13.159
Lehninger 13.056
Nozaki 12.676
DTASelect 12.661
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 12.237
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.031
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7903
0
7903
3310111
33
5821
418.8
47.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.393 ± 0.021
2.233 ± 0.024
5.065 ± 0.021
7.216 ± 0.035
3.919 ± 0.02
5.829 ± 0.038
2.518 ± 0.014
5.023 ± 0.024
6.511 ± 0.034
9.696 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.236 ± 0.012
4.158 ± 0.017
5.215 ± 0.034
4.654 ± 0.03
5.191 ± 0.022
8.209 ± 0.04
5.394 ± 0.02
6.317 ± 0.024
1.196 ± 0.011
3.021 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here