Apaloderma vittatum (Bar-tailed trogon) 
Average proteome isoelectric point is 6.65 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 7903 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A091NNV1|A0A091NNV1_APAVI Uncharacterized protein (Fragment) OS=Apaloderma vittatum OX=57397 GN=N311_05124 PE=4 SV=1 
SS1 pKa = 7.12  RR2 pKa = 11.84  LTWLEE7 pKa = 3.51  NSGVGFSLDD16 pKa = 3.46  YY17 pKa = 9.24  PTISLHH23 pKa = 6.08  AVSRR27 pKa = 11.84  DD28 pKa = 3.0  LSAYY32 pKa = 8.18  PWEE35 pKa = 4.42  HH36 pKa = 6.79  LYY38 pKa = 11.74  VMVNAKK44 pKa = 10.3  FEE46 pKa = 4.48  EE47 pKa = 4.52  EE48 pKa = 4.12  TKK50 pKa = 10.07  EE51 pKa = 4.02  APVAEE56 pKa = 4.68  GEE58 pKa = 4.41  EE59 pKa = 4.46  EE60 pKa = 4.74  DD61 pKa = 6.13  DD62 pKa = 5.21  SDD64 pKa = 6.0  DD65 pKa = 4.07  DD66 pKa = 4.36  SEE68 pKa = 5.15  PIAEE72 pKa = 4.16  FRR74 pKa = 11.84  FVPSDD79 pKa = 3.36  KK80 pKa = 10.88  SALEE84 pKa = 3.78  AMFAAMCEE92 pKa = 4.38  CQALHH97 pKa = 7.41  PDD99 pKa = 4.21  PDD101 pKa = 5.77  DD102 pKa = 4.05  EE103 pKa = 7.61  DD104 pKa = 4.46  SDD106 pKa = 3.76  NDD108 pKa = 3.97  YY109 pKa = 11.37  EE110 pKa = 4.59  GEE112 pKa = 4.16  EE113 pKa = 4.41  YY114 pKa = 10.56  DD115 pKa = 4.4  VEE117 pKa = 4.26  AHH119 pKa = 5.75  EE120 pKa = 5.4  LEE122 pKa = 5.33  HH123 pKa = 8.4  GDD125 pKa = 3.1  IPSFYY130 pKa = 9.42  TYY132 pKa = 11.03  EE133 pKa = 4.17  EE134 pKa = 4.58  GLSHH138 pKa = 6.9  LTAEE142 pKa = 4.71  GQATLEE148 pKa = 4.11  RR149 pKa = 11.84  LEE151 pKa = 4.74  GMLAQSVSSQYY162 pKa = 11.66  NMAGVRR168 pKa = 11.84  TEE170 pKa = 3.88  DD171 pKa = 3.84  SIRR174 pKa = 11.84  EE175 pKa = 4.08  YY176 pKa = 10.91  EE177 pKa = 4.67  DD178 pKa = 3.35  GMEE181 pKa = 4.02  VDD183 pKa = 3.84  VAPVVAGQFEE193 pKa = 4.42  DD194 pKa = 5.26  AEE196 pKa = 4.32  VDD198 pKa = 3.59  HH199 pKa = 7.08   
 Molecular weight: 22.27 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.72 
IPC2_protein 3.821 
IPC_protein 3.808 
Toseland    3.617 
ProMoST     3.923 
Dawson      3.77 
Bjellqvist  3.923 
Wikipedia   3.656 
Rodwell     3.63 
Grimsley    3.528 
Solomon     3.757 
Lehninger   3.719 
Nozaki      3.872 
DTASelect   4.037 
Thurlkill   3.643 
EMBOSS      3.668 
Sillero     3.923 
Patrickios  1.202 
IPC_peptide 3.757 
IPC2_peptide  3.897 
IPC2.peptide.svr19  3.802 
 Protein with the highest isoelectric point: 
>tr|A0A091NKP0|A0A091NKP0_APAVI 6-phosphofructo-2-kinase/fructose-2  6-bisphosphatase 2 (Fragment) OS=Apaloderma vittatum OX=57397 GN=N311_11513 PE=4 SV=1 
GG1 pKa = 7.26  KK2 pKa = 10.29  GRR4 pKa = 11.84  EE5 pKa = 4.21  GKK7 pKa = 10.25  GRR9 pKa = 11.84  AGKK12 pKa = 10.14  GRR14 pKa = 11.84  AGKK17 pKa = 10.12  GRR19 pKa = 11.84  AGKK22 pKa = 10.12  GRR24 pKa = 11.84  AGKK27 pKa = 10.12  GRR29 pKa = 11.84  AGKK32 pKa = 10.12  GRR34 pKa = 11.84  AGKK37 pKa = 10.12  GRR39 pKa = 11.84  AGKK42 pKa = 10.12  GRR44 pKa = 11.84  AGKK47 pKa = 10.12  GRR49 pKa = 11.84  AGKK52 pKa = 10.14  GRR54 pKa = 11.84  AGKK57 pKa = 10.28  GG58 pKa = 3.09   
 Molecular weight: 5.48 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.431 
IPC2_protein 10.935 
IPC_protein 12.501 
Toseland    12.676 
ProMoST     13.159 
Dawson      12.676 
Bjellqvist  12.661 
Wikipedia   13.144 
Rodwell     12.515 
Grimsley    12.72 
Solomon     13.159 
Lehninger   13.056 
Nozaki      12.676 
DTASelect   12.661 
Thurlkill   12.676 
EMBOSS      13.173 
Sillero     12.676 
Patrickios  12.237 
IPC_peptide 13.159 
IPC2_peptide  12.149 
IPC2.peptide.svr19  9.031 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        7903 
 
        
        0
 
        
        7903 
         
        3310111
 
        33
 
        5821
 
        418.8
 
        47.09
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        6.393 ± 0.021
2.233 ± 0.024
 
        5.065 ± 0.021
7.216 ± 0.035
 
        3.919 ± 0.02
5.829 ± 0.038
 
        2.518 ± 0.014
5.023 ± 0.024
       
        6.511 ± 0.034
9.696 ± 0.043
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.236 ± 0.012
4.158 ± 0.017
 
        5.215 ± 0.034
4.654 ± 0.03
 
        5.191 ± 0.022
8.209 ± 0.04
 
        5.394 ± 0.02
6.317 ± 0.024
       
        1.196 ± 0.011
3.021 ± 0.018
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here